Thermo Scientific™ Pierce™ RNA 3' End Desthiobiotinylation Kit

Description

• Perform easy and efficient desthiobiotin labeling of RNA for use as probes or as antibody alternatives for enrichment of RNA binding proteins with Thermo Scientific™ Pierce RNA 3' End Desthiobiotinylation Kit
• Additional Information and Online Ordering The Pierce RNA 3'-End Desthiobiotinylation Kit is optimized for labeling the 3'-end of single-stranded RNA using T4 RNA ligase
• Once labeled, the RNA can be used as a probe or target for gel-shift EMSA reactions, Northern blots and protein-RNA interaction experiments
• The desthiobiotinylated RNA also can be used to enrich for RNA binding proteins (RBP) using streptavidin affinity resin, because the desthiobiotin tag binds to streptavidin in a manner which allows gentle elution of the ribonucleoprotein complex
• The labeling kit is also included as a component of the Thermo Scientific Pierce Magnetic RNA-Protein Pull-Down Kit
• Highlights: Non-radioactive – a single biotin labels has similar detection sensitivity as radioactivity Easy to use – RNA ligase and optimized reaction buffer are included Economical – only a fraction of the cost purchasing synthetic biotinylated RNA probes End-labeled – results in minimal disturbance of RNA secondary structure Flexible – desthiobiotin label may be used for detection or as an affinity handle for streptavidin resin Includes: Desthiobiotinylated cytidine bisphosphate, T4 RNA ligase and buffer, unlabeled RNA oligonucleotide for use as a positive control, a biotinylated RNA probe standard, RNase inhibitor, glycogen, and ligation enhancing reagents Recommended for: Northern blotting EMSA Enrichment of RNA binding proteins (RBP)