PI17075
Thermo Scientific™ North2South™ Biotin Random Prime DNA Labeling Kit
Manufacturer: Thermo Scientific™
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Content And Storage
Sufficient For: 10 biotin-labeling reactions (each with 100 ng DNA)• Heptanucleotide Mix (5X), 100 μL• Deoxynucleotide Mix (5X), 80 μL• Reaction Buffer (10X), 50 μL• DNA Polymerase Klenow Fragment (5U/μL), 10 μL• Control DNA (250 ng/μL), 5 μL• Ammonium Acetate (5M), 1 mL• EDTA (0.5M, pH 8.0), 1 mL• Nuclease-free Water, 1 mL• Biotin-11-dUTP, 20 μLStore all components at -20°C.
Labeling Target
DNA
Product Type
DNA Labeling Kit
Format
Kit
Product Line
North2South™
Quantity
10 Reactions
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Description
- Thermo Scientific™ North2South Random Prime DNA Biotinylation Kit uses random heptanucleotides, Klenow fragment and biotin-nucleotides to produce biotinylated DNA templates for DNA hybridization and detection methods
- Random heptanucleotides containing all possible sequences are annealed to a denatured DNA template
- These act as primers for complementary strand synthesis by DNA polymerase
- Biotinylated dNTPs in the reaction mix are incorporated into the newly synthesized DNA
- This protocol yields biotin-labeled DNA of high activity for use as probes in hybridization experiments such as Southern and Northern hybridizations
- The resulting hybrids can be detected with streptavidin-horseradish peroxidase (HRP)
- Highlights: Ultimate sensitivity - labels with biotinylated dUTP, maximizing streptavidin-HRP binding and increasing sensitivity Works with as little as 100ng starting DNA template Exonuclease-free Klenow fragment produces higher yields Each reaction yields probe sufficient for approximately three (10 x 10cm) blots
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Content And Storage: Sufficient For: 10 biotin-labeling reactions (each with 100 ng DNA)• Heptanucleotide Mix (5X), 100 μL• Deoxynucleotide Mix (5X), 80 μL• Reaction Buffer (10X), 50 μL• DNA Polymerase Klenow Fragment (5U/μL), 10 μL• Control DNA (250 ng/μL), 5 μL• Ammonium Acetate (5M), 1 mL• EDTA (0.5M, pH 8.0), 1 mL• Nuclease-free Water, 1 mL• Biotin-11-dUTP, 20 μLStore all components at -20°C.
Labeling Target: DNA
Product Type: DNA Labeling Kit
Format: Kit
Product Line: North2South™
Quantity: 10 Reactions
Content And Storage:
Sufficient For: 10 biotin-labeling reactions (each with 100 ng DNA)• Heptanucleotide Mix (5X), 100 μL• Deoxynucleotide Mix (5X), 80 μL• Reaction Buffer (10X), 50 μL• DNA Polymerase Klenow Fragment (5U/μL), 10 μL• Control DNA (250 ng/μL), 5 μL• Ammonium Acetate (5M), 1 mL• EDTA (0.5M, pH 8.0), 1 mL• Nuclease-free Water, 1 mL• Biotin-11-dUTP, 20 μLStore all components at -20°C.
Labeling Target:
DNA
Product Type:
DNA Labeling Kit
Format:
Kit
Product Line:
North2South™
Quantity:
10 Reactions
Content And Storage: Sufficient For: 1000 cm2of membrane (10 mini-gel blots)• Stabilized Streptavidin-HRP, 1.5 mL• Luminol/Enhancer Solution, 80 mL• Stable Peroxide Solution, 80 mL• Hybridization Buffer, 125 mL• Hybridization Stringency Wash Buffer (2X), 375 mL• Blocking Buffer, 500 mL• Wash Buffer (4X), 500 mL• Substrate Equilibration Buffer, 500 mLUpon receipt, store kit at 4°C.
Labeling Target: __
Product Type: Hybridization and Detection Kit
Format: Kit
Product Line: North2South™
Quantity: 2160 mL Kit
Content And Storage:
Sufficient For: 1000 cm2of membrane (10 mini-gel blots)• Stabilized Streptavidin-HRP, 1.5 mL• Luminol/Enhancer Solution, 80 mL• Stable Peroxide Solution, 80 mL• Hybridization Buffer, 125 mL• Hybridization Stringency Wash Buffer (2X), 375 mL• Blocking Buffer, 500 mL• Wash Buffer (4X), 500 mL• Substrate Equilibration Buffer, 500 mLUpon receipt, store kit at 4°C.
Labeling Target:
__
Product Type:
Hybridization and Detection Kit
Format:
Kit
Product Line:
North2South™
Quantity:
2160 mL Kit
Content And Storage: Sufficient For: 10 biotin-labeling reactions (each with 100 ng DNA) and 10 mini-gel blots• North2South Chemiluminescent Hybridization and Detection Kit (Part No. 17097), 1 kit• North2South Biotin Random Prime Labeling Kit (Part No. 17075), 1 kit
Labeling Target: __
Product Type: Biotin Labeling and Detection Kit
Format: Kit
Product Line: North2South™
Quantity: 2164 mL Kit
Content And Storage:
Sufficient For: 10 biotin-labeling reactions (each with 100 ng DNA) and 10 mini-gel blots• North2South Chemiluminescent Hybridization and Detection Kit (Part No. 17097), 1 kit• North2South Biotin Random Prime Labeling Kit (Part No. 17075), 1 kit
Labeling Target:
__
Product Type:
Biotin Labeling and Detection Kit
Format:
Kit
Product Line:
North2South™
Quantity:
2164 mL Kit
Content And Storage: Store at room temperature.
Labeling Target: __
Product Type: DNA Coating Solution
Format: __
Product Line: __
Quantity: 100 mL
Content And Storage:
Store at room temperature.
Labeling Target:
__
Product Type:
DNA Coating Solution
Format:
__
Product Line:
__
Quantity:
100 mL