Thermo Scientific™ Pierce™ Biotin 3' End DNA Labeling Kit

Description

• Thermo Scientific™ Pierce Biotin 3' End DNA Labeling Kit is for tagging single-stranded DNA primers with biotin for use in non-radioactive electrophoretic mobility shift assays (EMSA) and other nucleic acid detection methods
• TdT exhibits a substrate preference of single-stranded DNA, but it will label duplex DNA with 3' overhangs and blunt duplexes, albeit with a lower efficiency
• The Biotin 3' End DNA Labeling Kit has been optimized to incorporate 1-3 biotinylated ribonucleotides (biotin-11-UTP) onto the 3' end of DNA strands
• This labeling strategy has the advantage of localizing the biotin to the 3' end of the probe where it will be less likely to interfere with hybridization or sequence-specific binding of proteins
• Biotin-labeled DNA probes can be used to facilitate non-isotopic detection in a variety of applications including electrophoretic mobility shift assays (EMSA), Northern or Southern blots, colony hybridizations, or in situ hybridizations
• Highlights: Non-isotopic labeling eliminates the hassle of hazardous radioactive materials or difficult-to-dispose-of waste 1-3 biotinylated ribonucleotides onto the 3' end of DNA strands for less interference with hybridization or sequence-specific binding of proteins Biotin-labeled probes are stable for more than one year 30-minute labeling procedure is fast and efficient Includes: 1mL 5X TdT Reaction Buffer, 50μL Terminal Deoxynucleotidyl Transferase, 100microliter Biotin-11-dUTP, 140μL Unlabeled Control Oligo, 40μL Biotin-Control Oligo