PI20148
Thermo Scientific™ LightShift™ Chemiluminescent EMSA Kit
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI20148-Each-of-1 | In Stock | ₹ 74,608.32 |
PI20148 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 74,608.32
GST (18%): ₹ 13,429.498
Total Price: ₹ 88,037.818
Formulation
Multi-component kit with reagents for DNA-protein binding reactions and blot detection
Includes
LightShift Optimization and Control Kit (20148X), 1 kit; Chemiluminescent Nucleic Acid Detection Module (89880), 1 kit
Product Type
LightShift Chemiluminescent EMSA Kit
For Use With (Application)
Electrophoretic mobility shift assays (EMSA) to identify and characterize protein-DNA binding interactions
No. of Reactions
100 binding reactions and detection on 800cm2of membrane (10 mini-gel blots)
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Description
- Thermo Scientific™ LightShift Chemiluminescent EMSA Kit is an extraordinarily robust and sensitive system for performing electrophoretic mobility shift assays (EMSA) to identify and characterize protein-DNA binding interactions
- The kit includes reagents for setting up and customizing protein:DNA binding reactions, a control set of DNA and protein extract to test the kit system, stabilized streptavidin-HRP conjugate to probe for the biotin-labeled DNA target, and an exceptionally sensitive chemiluminescent substrate module for detection.The principle for LightShift™ EMSA Detection is similar to a Western blot
- Biotin end-labeled duplex DNA is incubated with a nuclear extract or purified factor and electrophoresed on a native gel
- The DNA is then rapidly (30 minutes) transferred to a positive nylon membrane, UV-crosslinked, probed with streptavidin-HRP conjugate and incubated with the substrate
- The protocol from labeling to results can be accomplished in a single day
- All you need to perform the assay are purified DNA target that has been end-labeled with biotin, the protein extract you wish to test, nylon membrane and basic electrophoresis equipment
- DNA targets may be synthesized with 5' or 3' biotin labels or they may be labeled after synthesis using the Thermo Scientific Biotin 3' End DNA Labeling Kit (Part No
- 89818)
- Nuclear, cytosolic or whole cell protein extracts may be obtained by a variety of methods, including the Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Reagent Kit (Part No
- 78833)
- Highlights: Includes EBNA control system to help new users develop a working assay and understand the methods used to confirm binding interaction specificity Excellent for detecting low-abundance proteins in nuclear extracts Sensitivity that surpasses radioactive and digoxigenin methods Compatible with previously established binding conditions for popular DNA:protein interactions
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Formulation: Multi-component kit with reagents for DNA-protein binding reactions and blot detection
Includes: LightShift Optimization and Control Kit (20148X), 1 kit; Chemiluminescent Nucleic Acid Detection Module (89880), 1 kit
Product Type: LightShift Chemiluminescent EMSA Kit
For Use With (Application): Electrophoretic mobility shift assays (EMSA) to identify and characterize protein-DNA binding interactions
No. of Reactions: 100 binding reactions and detection on 800cm2of membrane (10 mini-gel blots)
Formulation:
Multi-component kit with reagents for DNA-protein binding reactions and blot detection
Includes:
LightShift Optimization and Control Kit (20148X), 1 kit; Chemiluminescent Nucleic Acid Detection Module (89880), 1 kit
Product Type:
LightShift Chemiluminescent EMSA Kit
For Use With (Application):
Electrophoretic mobility shift assays (EMSA) to identify and characterize protein-DNA binding interactions
No. of Reactions:
100 binding reactions and detection on 800cm2of membrane (10 mini-gel blots)
Formulation: __
Includes: __
Product Type: Poly (dI-dC) for LightShift EMSA Kit
For Use With (Application): __
No. of Reactions: __
Formulation:
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Includes:
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Product Type:
Poly (dI-dC) for LightShift EMSA Kit
For Use With (Application):
__
No. of Reactions:
__
Formulation: __
Includes: 10X Binding Buffer (1 mL), Biotin-EBNA Control DNA (50 μL), Unlabeled EBNA DNA (50 μL), EBNA Extract (125 μL), Poly dI·dC (125 μL), 50% Glycerol (500 μL), 1% NP-40 (500 μL), 1M KCl (1 mL), 100mM MgCl2(500 μL), 200mM EDTA pH 8.0 (500 μL), 5X Loading Buffer (1 mL)
Product Type: LightShift EMSA Optimization and Control Kit
For Use With (Application): __
No. of Reactions: __
Formulation:
__
Includes:
10X Binding Buffer (1 mL), Biotin-EBNA Control DNA (50 μL), Unlabeled EBNA DNA (50 μL), EBNA Extract (125 μL), Poly dI·dC (125 μL), 50% Glycerol (500 μL), 1% NP-40 (500 μL), 1M KCl (1 mL), 100mM MgCl2(500 μL), 200mM EDTA pH 8.0 (500 μL), 5X Loading Buffer (1 mL)
Product Type:
LightShift EMSA Optimization and Control Kit
For Use With (Application):
__
No. of Reactions:
__
Formulation: __
Includes: __
Product Type: Detergent
For Use With (Application): __
No. of Reactions: __
Formulation:
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Includes:
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Product Type:
Detergent
For Use With (Application):
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