PI17175

Thermo Scientific™ North2South™ Complete Biotin Random Prime Labeling and Detection Kit

Manufacturer: Thermo Scientific™

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Content And Storage

Sufficient For: 10 biotin-labeling reactions (each with 100 ng DNA) and 10 mini-gel blots• North2South Chemiluminescent Hybridization and Detection Kit (Part No. 17097), 1 kit• North2South Biotin Random Prime Labeling Kit (Part No. 17075), 1 kit

Format

Kit

Product Type

Biotin Labeling and Detection Kit

Detection Method

Chromogenic

Product Line

North2South™

Quantity

2164 mL Kit

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Description

  • Thermo Scientific™ North2South Chemiluminescent Detection Kit combines a novel ECL substrate for horseradish peroxidase (HRP) and optimized hybridization and blocking buffers to ensure consistent, high-sensitivity Southern blot results
  • The Thermo Scientific North2South Hybridization and Detection Kit combines a novel enhanced luminol substrate for horseradish peroxidase (HRP) with optimized pre-made hybridization and blocking buffers to ensure consistent results and high sensitivity
  • Any nucleic acid blot can be hybridized with the appropriate biotinylated (biotin-labeled) oligonucleotide probe
  • After a brief membrane blocking step, the biotin label is detected with streptavidin-HRP and a 5-minute incubation with the chemiluminescent substrate
  • Results are easily recorded by brief exposure to X-ray film (1-15 minutes) or CCD camera
  • Highlights: Sensitivity equal to or greater than 32 P Amenable to many formats High level of light output and consistent low background allows for very short exposure times using cooled CCD camera systems or film Ready-to-use buffers and short process time makes switching from radioactive detection to chemiluminescent detection an easy transition, even for the novice user

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Content And Storage:
Sufficient For: 10 biotin-labeling reactions (each with 100 ng DNA) and 10 mini-gel blots• North2South Chemiluminescent Hybridization and Detection Kit (Part No. 17097), 1 kit• North2South Biotin Random Prime Labeling Kit (Part No. 17075), 1 kit

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Kit

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Biotin Labeling and Detection Kit

Detection Method:
Chromogenic

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North2South™

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2164 mL Kit

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