20-159-MI

Thermo Scientific™ tRNA for LightShift™ Chemiluminescent RNA EMSA Kit

Manufacturer: Thermo Scientific™

Select a Size

Pack Size SKU Availability Price
Each of 1 20-159-MI-Each-of-1 In Stock ₹ 11,302.48

20-159-MI - Each of 1

₹ 11,302.48

In Stock

Quantity

1

Base Price: ₹ 11,302.48

GST (18%): ₹ 2,034.446

Total Price: ₹ 13,336.926

Content And Storage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above -70°C.

Format

Kit

Product Type

tRNA for LightShift RNA EMSA Kit

Target Specificity

Not Target-Specific

Detection Method

Chemiluminescence

Product Line

LightShift™

Quantity

100 μL

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Description

  • Thermo Scientific™ LightShift Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA)
  • The RNA EMSA Kit uses biotinylated RNA probes and a chemiluminescent substrate system to achieve fast and safe detection of RNA-protein complexes with sensitivity equivalent to traditional 32 P-isotopic methods
  • The complete kit comes with all reagents required to set up and optimize protein-RNA binding conditions, a positive control for protein-RNA interactions and reagents for chemiluminescent detection of the nucleic acid interaction
  • Highlights: Sensitive – chemiluminescent detection comparable to radioactive detection Time-saving – develop X-ray films after 1- to 5-minute exposures, versus 16 hour exposures needed with radioactive systems Flexible – compatible with RNA probes biotinylated by different methods Easy to use – complete kit includes optimized reagents for binding reactions and RNA probe detection Non-radioactive – eliminate waste concerns from radioactive RNA probes

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Thermo Scientific™

20-159-MI

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Content And Storage:
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above -70°C.

Format:
Kit

Product Type:
tRNA for LightShift RNA EMSA Kit

Target Specificity:
Not Target-Specific

Detection Method:
Chemiluminescence

Product Line:
LightShift™

Quantity:
100 μL

Img

Sigma Aldrich

20-160

1M Tris-HCl, pH 6.5....


Content And Storage:
__

Format:
__

Product Type:
__

Target Specificity:
__

Detection Method:
__

Product Line:
__

Quantity:
__

Img

Sigma Aldrich

20-160

1M Tris-HCl, pH 6.5....


Content And Storage:
__

Format:
__

Product Type:
__

Target Specificity:
__

Detection Method:
__

Product Line:
__

Quantity:
__

Img

Thermo Scientific™

20-160-MI

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Content And Storage:
Sufficient For: 20 labeling reactions (each with 50 pmol RNA)• T4 RNA Ligase Reaction Buffer (10X), 100 μL• T4 RNA Ligase (20,000 U/mL), 40 μL• Non-labeled RNA Control (10 μM), 100 μL• Biotinylated IRE RNA Control (125 nM), 35 μL• RNase Inhibitor (40 U/μL), 2 × 10 μL• Nuclease-free Water, 1.5 mL• DMSO, 200 μL• 30% PEG, 300 μL• Biotinylated Cytidine (Bis)phosphate (1mM), 40 μL• Glycogen (20 mg/mL), 20 μLStore all components at -20°C

Format:
Kit

Product Type:
RNA Labeling Kit

Target Specificity:
__

Detection Method:
__

Product Line:
Pierce™

Quantity:
20 Reactions