PI20148E
Thermo Scientific™ LightShift™ Poly (dI-dC)
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI20148E-Each-of-1 | In Stock | ₹ 12,602.40 |
PI20148E - Each of 1
In Stock
Quantity
1
Base Price: ₹ 12,602.40
GST (18%): ₹ 2,268.432
Total Price: ₹ 14,870.832
Assay
EMSA Assay
Product Line
LightShift™
Quantity
125 μL
Technique
Gel Shift
Content And Storage
Store at -20°C.
Product Type
Poly (dI-dC) for LightShift EMSA Kit
Target Specificity
Not Target-Specific
Related Products
Description
- Thermo Scientific™ LightShift Chemiluminescent EMSA Kit is an extraordinarily robust and sensitive system for performing electrophoretic mobility shift assays (EMSA) to identify and characterize protein-DNA binding interactions
- The kit includes reagents for setting up and customizing protein:DNA binding reactions, a control set of DNA and protein extract to test the kit system, stabilized streptavidin-HRP conjugate to probe for the biotin-labeled DNA target, and an exceptionally sensitive chemiluminescent substrate module for detection.The principle for LightShift™ EMSA Detection is similar to a Western blot
- Biotin end-labeled duplex DNA is incubated with a nuclear extract or purified factor and electrophoresed on a native gel
- The DNA is then rapidly (30 minutes) transferred to a positive nylon membrane, UV-crosslinked, probed with streptavidin-HRP conjugate and incubated with the substrate
- The protocol from labeling to results can be accomplished in a single day
- All you need to perform the assay are purified DNA target that has been end-labeled with biotin, the protein extract you wish to test, nylon membrane and basic electrophoresis equipment
- DNA targets may be synthesized with 5' or 3' biotin labels or they may be labeled after synthesis using the Thermo Scientific Biotin 3' End DNA Labeling Kit (Part No
- 89818)
- Nuclear, cytosolic or whole cell protein extracts may be obtained by a variety of methods, including the Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Reagent Kit (Part No
- 78833)
- Highlights: Includes EBNA control system to help new users develop a working assay and understand the methods used to confirm binding interaction specificity Excellent for detecting low-abundance proteins in nuclear extracts Sensitivity that surpasses radioactive and digoxigenin methods Compatible with previously established binding conditions for popular DNA:protein interactions
Compare Similar Items
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Assay: EMSA Assay
Product Line: LightShift™
Quantity: 125 μL
Technique: Gel Shift
Content And Storage: Store at -20°C.
Product Type: Poly (dI-dC) for LightShift EMSA Kit
Target Specificity: Not Target-Specific
Assay:
EMSA Assay
Product Line:
LightShift™
Quantity:
125 μL
Technique:
Gel Shift
Content And Storage:
Store at -20°C.
Product Type:
Poly (dI-dC) for LightShift EMSA Kit
Target Specificity:
Not Target-Specific
Assay: EMSA Assay
Product Line: LightShift™
Quantity: 100 Reactions
Technique: Gel Shift
Content And Storage: Sufficient For: 100 binding reactions• 10X Binding Buffer, 1 mL• Biotin-EBNA Control DNA, 50 μL• Unlabeled EBNA DNA, 50 μL• EBNA Extract, 125 μL• Poly (dI·dC), 125 μL• 50% Glycerol, 500 μL• 1% NP-40, 500 μL• 1 M KCl, 1 mL• 100mM MgCl2, 500 μL• 200mM EDTA pH 8.0, 500 μL• 5X Loading Buffer, 1 mLStore at -20°C.
Product Type: LightShift EMSA Optimization and Control Kit
Target Specificity: Not Target-Specific
Assay:
EMSA Assay
Product Line:
LightShift™
Quantity:
100 Reactions
Technique:
Gel Shift
Content And Storage:
Sufficient For: 100 binding reactions• 10X Binding Buffer, 1 mL• Biotin-EBNA Control DNA, 50 μL• Unlabeled EBNA DNA, 50 μL• EBNA Extract, 125 μL• Poly (dI·dC), 125 μL• 50% Glycerol, 500 μL• 1% NP-40, 500 μL• 1 M KCl, 1 mL• 100mM MgCl2, 500 μL• 200mM EDTA pH 8.0, 500 μL• 5X Loading Buffer, 1 mLStore at -20°C.
Product Type:
LightShift EMSA Optimization and Control Kit
Target Specificity:
Not Target-Specific
Assay: __
Product Line: Brij™, Surfact-Amps™
Quantity: 950 mL
Technique: __
Content And Storage: Store at Room Temperature
Product Type: Detergent
Target Specificity: __
Assay:
__
Product Line:
Brij™, Surfact-Amps™
Quantity:
950 mL
Technique:
__
Content And Storage:
Store at Room Temperature
Product Type:
Detergent
Target Specificity:
__
Assay: __
Product Line: Pierce™
Quantity: 20 Reactions
Technique: __
Content And Storage: Sufficient For: 20 desthiobiotinylation reactions using 50 pmol of RNA each• Desthiobiotinylated Cytidine Bisphosphate, 40 μL• T4 RNA Ligase, 40 μL• T4 RNA Ligase Reaction buffer (10X), 100 μL• Non-labeled RNA Control, 100 μL• Biotinylated IRE RNA Control, 35 μL• RNase Inhibitor, 2 x 10 μL• DMSO, 200 μL• PEG (30%), 300 μL• Glycogen, 20 μL• Nuclease-free Water, 1.5 mLUpon receipt, store at -20°C.
Product Type: RNA Labeling Kit
Target Specificity: __
Assay:
__
Product Line:
Pierce™
Quantity:
20 Reactions
Technique:
__
Content And Storage:
Sufficient For: 20 desthiobiotinylation reactions using 50 pmol of RNA each• Desthiobiotinylated Cytidine Bisphosphate, 40 μL• T4 RNA Ligase, 40 μL• T4 RNA Ligase Reaction buffer (10X), 100 μL• Non-labeled RNA Control, 100 μL• Biotinylated IRE RNA Control, 35 μL• RNase Inhibitor, 2 x 10 μL• DMSO, 200 μL• PEG (30%), 300 μL• Glycogen, 20 μL• Nuclease-free Water, 1.5 mLUpon receipt, store at -20°C.
Product Type:
RNA Labeling Kit
Target Specificity:
__