15-596-018
Invitrogen™ TRIzol™ Reagent
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 15-596-018-Each-of-1 | In Stock | ₹ 37,560.84 |
15-596-018 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 37,560.84
GST (18%): ₹ 6,760.951
Total Price: ₹ 44,321.791
Content And Storage
200 mL TRIzol Reagent; room temperature
Purification Time
1 hr.
Sample Type
Bacteria, Cells, Plants, Tissue, Yeast
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Quantity
200 mL
Starting Material Amount
Bacteria: ≤107cellsCells: ≤107Plant: ≤107cells, 100 mgTissue: ≤100 mgYeast: ≤107cells
Isolation Technology
Organic Extraction
Elution Volume
20 to 600 μL
Final Product Type
Total RNA, DNA, Protein
For Use With (Application)
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern/Western blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization, enzymatic assays, immunoprecipitation, gel shift assays, 2D gel electrophoresis
Shipping Condition
Room Temperature
Yield
DNA: ≤7 μgRNA: ≤73 μgProtein: varies
Related Products
Description
- TRIzol™ Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples
- This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour
- Key features of TRIzol™ Reagent include: • Permits the isolation of RNA, DNA, and protein from the same sample • Offers superior lysis capability, even with difficult sample types • Optimized formulations and protocols for tissues, cells, serum, virus, and bacteria Reliably purify RNA from multiple sample volumes and sources TRIzol™ Reagent performs well with small quantities of tissue (50–100 mg) and cells (5 × 10 6 ), as well as with large quantities of tissue (≥1 g) and cells (>10 7 ), and comes with prototcols for purification from samples of human, animal, plant, or bacterial origin
- TRIzol™ Reagent maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting cells and dissolving cell components during sample homogenization
- The simplicity of the TRIzol™ Reagent method allows simultaneous processing of a large number of samples
- The entire procedure can be completed in 1 hour
- Total RNA isolated by TRIzol™ Reagent is free of protein and DNA contamination
- Formulated for isolation of multiple molecular targets TRIzol™ Reagent allows you to perform sequential precipitation of RNA, DNA, and proteins from a single sample
- After homogenizing the sample with TRIzol™ Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), and interphase and red lower organic layers (containing the DNA and proteins)
- RNA is precipitated from the aqueous layer with isopropanol
- DNA is precipitated from the interphase/organic layer with ethanol
- Protein is precipitated from the phenol-ethanol supernatant by isopropanol precipitation
- The precipitated RNA, DNA, or protein is washed to remove impurities, and then resuspended for use in downstream applications
- Order Info Shipping Condition: Room temperature
Compare Similar Items
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Content And Storage: 200 mL TRIzol Reagent; room temperature
Purification Time: 1 hr.
Sample Type: Bacteria, Cells, Plants, Tissue, Yeast
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 200 mL
Starting Material Amount: Bacteria: ≤107cellsCells: ≤107Plant: ≤107cells, 100 mgTissue: ≤100 mgYeast: ≤107cells
Isolation Technology: Organic Extraction
Elution Volume: 20 to 600 μL
Final Product Type: Total RNA, DNA, Protein
For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern/Western blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization, enzymatic assays, immunoprecipitation, gel shift assays, 2D gel electrophoresis
Shipping Condition: Room Temperature
Yield: DNA: ≤7 μgRNA: ≤73 μgProtein: varies
Content And Storage:
200 mL TRIzol Reagent; room temperature
Purification Time:
1 hr.
Sample Type:
Bacteria, Cells, Plants, Tissue, Yeast
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
200 mL
Starting Material Amount:
Bacteria: ≤107cellsCells: ≤107Plant: ≤107cells, 100 mgTissue: ≤100 mgYeast: ≤107cells
Isolation Technology:
Organic Extraction
Elution Volume:
20 to 600 μL
Final Product Type:
Total RNA, DNA, Protein
For Use With (Application):
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern/Western blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization, enzymatic assays, immunoprecipitation, gel shift assays, 2D gel electrophoresis
Shipping Condition:
Room Temperature
Yield:
DNA: ≤7 μgRNA: ≤73 μgProtein: varies
Content And Storage: 100 mL TRIzol Reagent; room temperature
Purification Time: 1 hr.
Sample Type: Bacteria, Cells, Plants, Tissue, Yeast
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 100 mL
Starting Material Amount: Bacteria: ≤107cellsCells: ≤107Plant: ≤107cells, 100 mgTissue: ≤100 mgYeast: ≤107cells
Isolation Technology: Organic Extraction
Elution Volume: 20 to 600 μL
Final Product Type: Total RNA, DNA, Protein
For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern/Western blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization, enzymatic assays, immunoprecipitation, gel shift assays, 2D gel electrophoresis
Shipping Condition: Room Temperature
Yield: DNA: ≤7 μgRNA: ≤73 μgProtein: varies
Content And Storage:
100 mL TRIzol Reagent; room temperature
Purification Time:
1 hr.
Sample Type:
Bacteria, Cells, Plants, Tissue, Yeast
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
100 mL
Starting Material Amount:
Bacteria: ≤107cellsCells: ≤107Plant: ≤107cells, 100 mgTissue: ≤100 mgYeast: ≤107cells
Isolation Technology:
Organic Extraction
Elution Volume:
20 to 600 μL
Final Product Type:
Total RNA, DNA, Protein
For Use With (Application):
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern/Western blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization, enzymatic assays, immunoprecipitation, gel shift assays, 2D gel electrophoresis
Shipping Condition:
Room Temperature
Yield:
DNA: ≤7 μgRNA: ≤73 μgProtein: varies
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Purification Time: __
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High-throughput Compatibility: __
Quantity: 100 Tests
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Content And Storage:
4° C, store in dark, DO NOT FREEZE!
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High-throughput Compatibility:
__
Quantity:
100 Tests
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