Invitrogen™ TRI Reagent™ Solution

Description

• Tri Reagent™ solution is a complete, ready-to-use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples
• Key features: • Simple —a single reagent for isolation of DNA-free RNA, RNA-free DNA, and protein • Productive —obtain higher yields than with traditional guanidine thiocyanate or cesium chloride methods • Versatile —works with many sample sources, including human, plant, yeast, bacterial and viral samples • Flexible —easily scalable depending on starting material and desired yield Using TRI Reagent™ solution TRI Reagent™ starting material can be either fresh or frozen tissue, or cell samples of almost any size—the protocol is easily scalable
• This RNA isolation method is well-recognized and referenced in numerous publications, a testament to its reliability
• How it works TRI Reagent™ combines phenol and guanidine thiocyanate in a monophase solution to facilitate the immediate inhibition of RNase activity
• Biological samples are homogenized or lysed in TRI Reagent™; the subsequent addition of bromochloropropane or chloroform results in the separation of the homogenate into aqueous and organic phases
• RNA partitions to the aqueous phase, DNA to the interphase, and proteins to the organic phase
• The RNA can then be precipitated from the aqueous phase with the addition of isopropanol
• The entire protocol can be completed in approximately 1 hour
• Applications The isolated RNA is suitable for any downstream application, including RT-PCR, amplification for array analysis, hybridization assays, or in vitro translation
• PCR, Southern blotting, and cloning can be performed using DNA isolated with TRI Reagent™
• Isolated proteins can be used in SDS-PAGE and western blotting
• Tri Reagent™ solution is incorporated into our RiboPure™ and MagMAX™-96 for Microarrays RNA isolation kits
• Order Info Shipping Condition: Room temperature