Invitrogen™ Dynabeads™ Oligo(dT)25

Description

• Dynabeads™ Oligo(dT) 25 mRNA isolation beads specifically target and capture mRNA molecules from virtually any crude sample and eliminate the need to purify total RNA when the desired information-bearing nucleic acid is mRNA
• Since mRNA comprises only about 1–5% ot total cellular RNA, the isolation of total RNA is not the most efficient way to isolate mRNA
• Other technologies designed to purify total RNA yield ∼80% ribosomal RNA and force mRNA to compete with ribosomal RNA, transfer RNA, micro RNA, small nucleolar RNA, and small cytoplasmic RNA for membrane binding
• Advantages of Dynabeads™ Oligo(dT) 25 beads: • Fast and gentle procedure yields pure intact mRNA • Extremely pure mRNA isolation, best choice upstream of cDNA synthesis • Exquisitely sensitive mRNA isolation enables cDNA synthesis and cDNA library construction from ultra-small starting samples (enables cDNA library construction from a single cell) How the beads work The oligo(dT) 25 -coated Dynabeads™ specifically target and capture the mRNA transcriptome from an extremely wide variety of crude starting samples
• Ribosomal RNA, DNA, proteins, and small RNA molecules (such as transfer RNA, micro RNA, and small nucleolar RNA) do not bind to the beads and are discarded
• Only polyadenylated RNA species (mRNA) are captured
• Isolated mRNA is pure, eliminating the need for ribosomal RNA subtraction or a post-extraction DNase treatment
• This column-free system ensures the highest transcriptome recovery: • Physical mRNA capture on mobile magnetic beads • Rapid and gentle magnetic handling procedures • No mRNA lost during high g-force spins • No mRNA trapped in column membranes during elution Applications mRNA is suitable for all downstream molecular applications, including gene cloning, cDNA synthesis, cDNA library construction, RT-PCR, quantitative RT-PCR, RPA (Ribonuclease Protection Assay), subtractive hybridization, primer extension, SAGE, RACE, and others
• The Dynabeads™ Oligo(dT) 25 mRNA isolation beads are the ideal mRNA purification method prior to cDNA library construction
• Use of these beads ensures the highest recovery and enrichment of the transcriptome
• These beads capture more of the transcriptome than is possible with methods that integrate a total RNA isolation step upstream of mRNA isolation
• Verastile elution options Elution can be performed in any volume down to 5 μL
• mRNA elution is optional because enzymatic reactions in downstream procedures are not inhibited by presence of Dynabeads™
• Additionally, one can perform cDNA synthesis directly on the beads to create a reusable solid-phase cDNA library
• Order Info Shipping Condition: Room Temperature