61-005
Invitrogen™ Dynabeads™ Oligo(dT)25
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 61-005-Each-of-1 | In Stock | ₹ 86,848.00 |
61-005 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 86,848.00
GST (18%): ₹ 15,632.64
Total Price: ₹ 1,02,480.64
Content And Storage
5 mL Dynabeads; 4°C, Store at 2°C to 8°C.
Purification Time
15 min.
Final Product Type
mRNA
For Use With (Application)
qPCR
Shipping Condition
Room Temperature
Yield
10 μg mRNA per 1 mL of beads (Binding capacity)
Isolation Technology
Magnetic Bead
Elution Volume
10–20 μL
High-throughput Compatibility
High-throughput Compatible
Quantity
5 mL
Starting Material Amount
Cells: ≤106Plant: ≤100 mgTissue: ≤50 mgTotal RNA: ≤75 μg
Ligand Type
Oligo-dT
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Description
- Description Dynabeads™ Oligo(dT) 25 mRNA isolation beads specifically target and capture mRNA molecules from virtually any crude sample and eliminate the need to purify total RNA when the desired information-bearing nucleic acid is mRNA
- Since mRNA comprises only about 1–5% ot total cellular RNA, the isolation of total RNA is not the most efficient way to isolate mRNA
- Other technologies designed to purify total RNA yield ∼80% ribosomal RNA and force mRNA to compete with ribosomal RNA, transfer RNA, micro RNA, small nucleolar RNA, and small cytoplasmic RNA for membrane binding
- Advantages of Dynabeads™ Oligo(dT) 25 beads: • Fast and gentle procedure yields pure intact mRNA • Extremely pure mRNA isolation, best choice upstream of cDNA synthesis • Exquisitely sensitive mRNA isolation enables cDNA synthesis and cDNA library construction from ultra-small starting samples (enables cDNA library construction from a single cell) How the beads work The oligo(dT) 25 -coated Dynabeads™ specifically target and capture the mRNA transcriptome from an extremely wide variety of crude starting samples
- Ribosomal RNA, DNA, proteins, and small RNA molecules (such as transfer RNA, micro RNA, and small nucleolar RNA) do not bind to the beads and are discarded
- Only polyadenylated RNA species (mRNA) are captured
- Isolated mRNA is pure, eliminating the need for ribosomal RNA subtraction or a post-extraction DNase treatment
- This column-free system ensures the highest transcriptome recovery: • Physical mRNA capture on mobile magnetic beads • Rapid and gentle magnetic handling procedures • No mRNA lost during high g-force spins • No mRNA trapped in column membranes during elution Applications mRNA is suitable for all downstream molecular applications, including gene cloning, cDNA synthesis, cDNA library construction, RT-PCR, quantitative RT-PCR, RPA (Ribonuclease Protection Assay), subtractive hybridization, primer extension, SAGE, RACE, and others
- The Dynabeads™ Oligo(dT) 25 mRNA isolation beads are the ideal mRNA purification method prior to cDNA library construction
- Use of these beads ensures the highest recovery and enrichment of the transcriptome
- These beads capture more of the transcriptome than is possible with methods that integrate a total RNA isolation step upstream of mRNA isolation
- Verastile elution options Elution can be performed in any volume down to 5 μL
- mRNA elution is optional because enzymatic reactions in downstream procedures are not inhibited by presence of Dynabeads™
- Additionally, one can perform cDNA synthesis directly on the beads to create a reusable solid-phase cDNA library
- Order Info Shipping Condition: Room Temperature
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Content And Storage: 5 mL Dynabeads; 4°C, Store at 2°C to 8°C.
Purification Time: 15 min.
Final Product Type: mRNA
For Use With (Application): qPCR
Shipping Condition: Room Temperature
Yield: 10 μg mRNA per 1 mL of beads (Binding capacity)
Isolation Technology: Magnetic Bead
Elution Volume: 10–20 μL
High-throughput Compatibility: High-throughput Compatible
Quantity: 5 mL
Starting Material Amount: Cells: ≤106Plant: ≤100 mgTissue: ≤50 mgTotal RNA: ≤75 μg
Ligand Type: Oligo-dT
Content And Storage:
5 mL Dynabeads; 4°C, Store at 2°C to 8°C.
Purification Time:
15 min.
Final Product Type:
mRNA
For Use With (Application):
qPCR
Shipping Condition:
Room Temperature
Yield:
10 μg mRNA per 1 mL of beads (Binding capacity)
Isolation Technology:
Magnetic Bead
Elution Volume:
10–20 μL
High-throughput Compatibility:
High-throughput Compatible
Quantity:
5 mL
Starting Material Amount:
Cells: ≤106Plant: ≤100 mgTissue: ≤50 mgTotal RNA: ≤75 μg
Ligand Type:
Oligo-dT
Content And Storage: • 2 mL Dynabeads Oligo (dT)25; 4°C• 5 mL Binding Buffer; 4°C• 5 mL Washing Buffer B; 4°C• 5 mL 10 mM Tris-HCl; 4°C
Purification Time: 10 min.
Final Product Type: mRNA
For Use With (Application): RT-PCR, qPCR, cDNA library construction, microarray analysis
Shipping Condition: Room Temperature
Yield: 2 μg mRNA per 200 μL of beads (Binding capacity)
Isolation Technology: Magnetic Bead
Elution Volume: 5 to 20 μL
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 2 mL
Starting Material Amount: ≤100 μL
Ligand Type: __
Content And Storage:
• 2 mL Dynabeads Oligo (dT)25; 4°C• 5 mL Binding Buffer; 4°C• 5 mL Washing Buffer B; 4°C• 5 mL 10 mM Tris-HCl; 4°C
Purification Time:
10 min.
Final Product Type:
mRNA
For Use With (Application):
RT-PCR, qPCR, cDNA library construction, microarray analysis
Shipping Condition:
Room Temperature
Yield:
2 μg mRNA per 200 μL of beads (Binding capacity)
Isolation Technology:
Magnetic Bead
Elution Volume:
5 to 20 μL
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
2 mL
Starting Material Amount:
≤100 μL
Ligand Type:
__
Content And Storage: • 5 mL Dynabeads oligo (dT)25• 30 mL Lysis/Binding Buffer• 60 mL Washing Buffer A• 30 mL Washing Buffer B• 15 mL 10 mM Tris-HCl (Elution buffer)Contains enough reagents for 20 standard isolations. Store at 2 to 8°C.
Purification Time: 15 min.
Final Product Type: mRNA
For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Nuclease Protection Assays, Northern Blotting, Cloning
Shipping Condition: Room Temperature
Yield: __
Isolation Technology: Magnetic Bead
Elution Volume: __
High-throughput Compatibility: High-throughput Compatible, Manual Protocols, Automated Protocols
Quantity: 5 mL
Starting Material Amount: __
Ligand Type: __
Content And Storage:
• 5 mL Dynabeads oligo (dT)25• 30 mL Lysis/Binding Buffer• 60 mL Washing Buffer A• 30 mL Washing Buffer B• 15 mL 10 mM Tris-HCl (Elution buffer)Contains enough reagents for 20 standard isolations. Store at 2 to 8°C.
Purification Time:
15 min.
Final Product Type:
mRNA
For Use With (Application):
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Nuclease Protection Assays, Northern Blotting, Cloning
Shipping Condition:
Room Temperature
Yield:
__
Isolation Technology:
Magnetic Bead
Elution Volume:
__
High-throughput Compatibility:
High-throughput Compatible, Manual Protocols, Automated Protocols
Quantity:
5 mL
Starting Material Amount:
__
Ligand Type:
__
Content And Storage: • 10 mL Dynabeads Oligo (dt)25, 4°C• 60 mL Lysis/Binding Buffer; 4°C• 120 mL Washing Buffer A; 4°C• 60 mL Washing Buffer B; 4°C• 15 mL 10 mM Tris-HCl; 4°C
Purification Time: __
Final Product Type: mRNA
For Use With (Application): RT-PCR, qPCR, cDNA library construction, microarray analysis
Shipping Condition: Room Temperature
Yield: 2 μg mRNA per 200 μL of beads (Binding capacity)
Isolation Technology: Magnetic Bead
Elution Volume: 10 to 100 μL
High-throughput Compatibility: High-throughput Compatible
Quantity: 10 mL
Starting Material Amount: Cells: ≤106Plant: ≤400 mgTissue: ≤200 mg
Ligand Type: __
Content And Storage:
• 10 mL Dynabeads Oligo (dt)25, 4°C• 60 mL Lysis/Binding Buffer; 4°C• 120 mL Washing Buffer A; 4°C• 60 mL Washing Buffer B; 4°C• 15 mL 10 mM Tris-HCl; 4°C
Purification Time:
__
Final Product Type:
mRNA
For Use With (Application):
RT-PCR, qPCR, cDNA library construction, microarray analysis
Shipping Condition:
Room Temperature
Yield:
2 μg mRNA per 200 μL of beads (Binding capacity)
Isolation Technology:
Magnetic Bead
Elution Volume:
10 to 100 μL
High-throughput Compatibility:
High-throughput Compatible
Quantity:
10 mL
Starting Material Amount:
Cells: ≤106Plant: ≤400 mgTissue: ≤200 mg
Ligand Type:
__