61-012
Invitrogen™ Dynabeads™ mRNA DIRECT™ Purification Kit
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 61-012-Each-of-1 | In Stock | ₹ 1,19,955.12 |
61-012 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,19,955.12
GST (18%): ₹ 21,591.922
Total Price: ₹ 1,41,547.042
Content And Storage
• 10 mL Dynabeads Oligo (dt)25, 4°C• 60 mL Lysis/Binding Buffer; 4°C• 120 mL Washing Buffer A; 4°C• 60 mL Washing Buffer B; 4°C• 15 mL 10 mM Tris-HCl; 4°C
Elution Volume
10 to 100 μL
Final Product Type
mRNA
For Use With (Equipment)
Automated Liquid Handling Systems
Green Features
Beads may be reused for multiple extractions
Shipping Condition
Room Temperature
Yield
2 μg mRNA per 200 μL of beads (Binding capacity)
Isolation Technology
Magnetic Bead
Sample Type
Cells, Plants, Plasma, Serum, Tissue, Total RNA, Blood
High-throughput Compatibility
High-throughput Compatible
For Use With (Application)
RT-PCR, qPCR, cDNA library construction, microarray analysis
Quantity
10 mL
Starting Material Amount
Cells: ≤106Plant: ≤400 mgTissue: ≤200 mg
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Description
- The Dynabeads™ mRNA DIRECT™ Kit is designed for simple and rapid isolation of pure, intact polyadenylated (polyA) mRNA directly from the crude lysate of animal and plant cells and tissues
- The isolated mRNA is suitable for use in all downstream applications
- Advantages of the Dynabeads mRNA DIRECT Kit: • Fast —15 minute procedure yields pure, intact mRNA • Highly pure mRNA isolation —best choice upstream of cDNA synthesis • Sensitive mRNA isolation —enables cDNA synthesis and cDNA library construction from ultra-small starting samples (enables cDNA library construction from a single cell) System overview The isolation protocol relies on base pairing between the polyA residues at the 3' end of most mRNA, and the oligo (dT) 25 residues covalently coupled to the surface of the Dynabeads
- Other RNA species lacking a polyA tail will not hybridize to the beads and are readily washed away
- Ribosomal RNA, DNA, proteins, and small RNA molecules (such as transfer RNA, micro RNA, and small nucleolar RNA) do not bind to the beads and are discarded
- RNase inhibiting agents in the Lysis/Binding Buffer together with stringent hybridization and washing conditions ensure the isolation of pure, intact mRNA from crude samples rich in RNase, without the use of strong chaotropic agents
- The mRNA purification beads specifically target and capture the mRNA transcriptome from an extremely wide variety of crude starting samples (see protocol)
- 1 mg of Dynabeads Oligo (dT) 25 beads (200 μL) binds up to 2 μg of mRNA
- A typical mammalian cell contains about 10–30 pg of total RNA, from which 1–5% is mRNA.
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Content And Storage: • 10 mL Dynabeads Oligo (dt)25, 4°C• 60 mL Lysis/Binding Buffer; 4°C• 120 mL Washing Buffer A; 4°C• 60 mL Washing Buffer B; 4°C• 15 mL 10 mM Tris-HCl; 4°C
Elution Volume: 10 to 100 μL
Final Product Type: mRNA
For Use With (Equipment): Automated Liquid Handling Systems
Green Features: Beads may be reused for multiple extractions
Shipping Condition: Room Temperature
Yield: 2 μg mRNA per 200 μL of beads (Binding capacity)
Isolation Technology: Magnetic Bead
Sample Type: Cells, Plants, Plasma, Serum, Tissue, Total RNA, Blood
High-throughput Compatibility: High-throughput Compatible
For Use With (Application): RT-PCR, qPCR, cDNA library construction, microarray analysis
Quantity: 10 mL
Starting Material Amount: Cells: ≤106Plant: ≤400 mgTissue: ≤200 mg
Content And Storage:
• 10 mL Dynabeads Oligo (dt)25, 4°C• 60 mL Lysis/Binding Buffer; 4°C• 120 mL Washing Buffer A; 4°C• 60 mL Washing Buffer B; 4°C• 15 mL 10 mM Tris-HCl; 4°C
Elution Volume:
10 to 100 μL
Final Product Type:
mRNA
For Use With (Equipment):
Automated Liquid Handling Systems
Green Features:
Beads may be reused for multiple extractions
Shipping Condition:
Room Temperature
Yield:
2 μg mRNA per 200 μL of beads (Binding capacity)
Isolation Technology:
Magnetic Bead
Sample Type:
Cells, Plants, Plasma, Serum, Tissue, Total RNA, Blood
High-throughput Compatibility:
High-throughput Compatible
For Use With (Application):
RT-PCR, qPCR, cDNA library construction, microarray analysis
Quantity:
10 mL
Starting Material Amount:
Cells: ≤106Plant: ≤400 mgTissue: ≤200 mg
Content And Storage: −20°C
Elution Volume: __
Final Product Type: __
For Use With (Equipment): __
Green Features: __
Shipping Condition: __
Yield: __
Isolation Technology: __
Sample Type: __
High-throughput Compatibility: __
For Use With (Application): __
Quantity: 50 μg
Starting Material Amount: __
Content And Storage:
−20°C
Elution Volume:
__
Final Product Type:
__
For Use With (Equipment):
__
Green Features:
__
Shipping Condition:
__
Yield:
__
Isolation Technology:
__
Sample Type:
__
High-throughput Compatibility:
__
For Use With (Application):
__
Quantity:
50 μg
Starting Material Amount:
__
Content And Storage: −20°C
Elution Volume: __
Final Product Type: __
For Use With (Equipment): __
Green Features: __
Shipping Condition: __
Yield: __
Isolation Technology: __
Sample Type: __
High-throughput Compatibility: __
For Use With (Application): __
Quantity: 50 μg
Starting Material Amount: __
Content And Storage:
−20°C
Elution Volume:
__
Final Product Type:
__
For Use With (Equipment):
__
Green Features:
__
Shipping Condition:
__
Yield:
__
Isolation Technology:
__
Sample Type:
__
High-throughput Compatibility:
__
For Use With (Application):
__
Quantity:
50 μg
Starting Material Amount:
__
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Elution Volume: __
Final Product Type: __
For Use With (Equipment): __
Green Features: __
Shipping Condition: __
Yield: __
Isolation Technology: __
Sample Type: __
High-throughput Compatibility: __
For Use With (Application): __
Quantity: 100 Tests
Starting Material Amount: __
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Elution Volume:
__
Final Product Type:
__
For Use With (Equipment):
__
Green Features:
__
Shipping Condition:
__
Yield:
__
Isolation Technology:
__
Sample Type:
__
High-throughput Compatibility:
__
For Use With (Application):
__
Quantity:
100 Tests
Starting Material Amount:
__