4918729
Invitrogen™ mRNA Catcher™ PLUS Purification Kit
The mRNA Catcher™ PLUS Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format.
Manufacturer: Fischer Scientific
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Isolation Technology
Oligo dT Affinity
Elution Volume
80 μL
Final Product Type
mRNA
For Use With (Equipment)
Robotic Liquid Handling Systems
Shipping Condition
Room Temperature
Yield
>80 ng
Purification Time
1.5 hr. (Including sample homogenization)
Sample Type
Cells, Tissue, Total RNA, Whole Blood
High-throughput Compatibility
High-throughput Compatible
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, Microarray Analysis
Starting Material Amount
Cells: 100–106Tissue: 4–40 mgTotal RNA: 0.1–100 μgWhole Blood: 40 μL
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Description
- The mRNA Catcher™ PLUS Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format
- By taking advantage of Locked Nucleic Acid (LNA) technology, the mRNA Catcher PLUS Kit provides improved performance and a faster protocol, while preserving mRNA yield and purity
- How it works mRNA from total RNA, cells, tissue, and blood samples is hybridized to an immobilized, single-stranded 20-mer oligo(dT)-containing LNA
- The incorporation of LNA into the oligo probe increases the hybridization efficiency and specificity of the mRNA binding
- The mRNA can either be eluted or directly reversed-transcribed to cDNA directly in the well
- The mRNA Catcher PLUS method does not require centrifugation, precipitation, phase separation, or column filtration
- In addition, the entire method is amenable to automation for streamlined, high-throughput expression profiling for large-scale processes
- Isolated mRNA is suitable for use in downstream applications such as RT-PCR and qRT-PCR
- • Automated protocols, high-throughput compatible • Robust performance: high binding capacity per well (>80ng/well) • Reproducible results: low CVs from well-to-well and plate-to-plate (∽ 2%) • Optimized protocols: for small sample sizes (100 cells, 4 mg tissue, and 40 μL blood) • Compatible plate design: may be used with most PCR cyclers Order Info Shipping Condition: Wet ice
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Isolation Technology: Oligo dT Affinity
Elution Volume: 80 μL
Final Product Type: mRNA
For Use With (Equipment): Robotic Liquid Handling Systems
Shipping Condition: Room Temperature
Yield: >80 ng
Purification Time: 1.5 hr. (Including sample homogenization)
Sample Type: Cells, Tissue, Total RNA, Whole Blood
High-throughput Compatibility: High-throughput Compatible
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, Microarray Analysis
Starting Material Amount: Cells: 100–106Tissue: 4–40 mgTotal RNA: 0.1–100 μgWhole Blood: 40 μL
Isolation Technology:
Oligo dT Affinity
Elution Volume:
80 μL
Final Product Type:
mRNA
For Use With (Equipment):
Robotic Liquid Handling Systems
Shipping Condition:
Room Temperature
Yield:
>80 ng
Purification Time:
1.5 hr. (Including sample homogenization)
Sample Type:
Cells, Tissue, Total RNA, Whole Blood
High-throughput Compatibility:
High-throughput Compatible
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, Microarray Analysis
Starting Material Amount:
Cells: 100–106Tissue: 4–40 mgTotal RNA: 0.1–100 μgWhole Blood: 40 μL
Isolation Technology: Organic Extraction
Elution Volume: 20 to 600 μL
Final Product Type: Total RNA, DNA, Protein
For Use With (Equipment): __
Shipping Condition: Room Temperature
Yield: DNA: ≤7 μgRNA: ≤73 μgProtein: varies
Purification Time: 1 hr.
Sample Type: Bacteria, Cells, Plants, Tissue, Yeast
High-throughput Compatibility: Not High-throughput Compatible (Manual)
For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern/Western blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization, enzymatic assays, immunoprecipitation, gel shift assays, 2D gel electrophoresis
Starting Material Amount: Bacteria: ≤107cellsCells: ≤107Plant: ≤107cells, 100 mgTissue: ≤100 mgYeast: ≤107cells
Isolation Technology:
Organic Extraction
Elution Volume:
20 to 600 μL
Final Product Type:
Total RNA, DNA, Protein
For Use With (Equipment):
__
Shipping Condition:
Room Temperature
Yield:
DNA: ≤7 μgRNA: ≤73 μgProtein: varies
Purification Time:
1 hr.
Sample Type:
Bacteria, Cells, Plants, Tissue, Yeast
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
For Use With (Application):
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern/Western blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization, enzymatic assays, immunoprecipitation, gel shift assays, 2D gel electrophoresis
Starting Material Amount:
Bacteria: ≤107cellsCells: ≤107Plant: ≤107cells, 100 mgTissue: ≤100 mgYeast: ≤107cells
Isolation Technology: __
Elution Volume: __
Final Product Type: __
For Use With (Equipment): __
Shipping Condition: Dry Ice
Yield: __
Purification Time: __
Sample Type: __
High-throughput Compatibility: __
For Use With (Application): __
Starting Material Amount: __
Isolation Technology:
__
Elution Volume:
__
Final Product Type:
__
For Use With (Equipment):
__
Shipping Condition:
Dry Ice
Yield:
__
Purification Time:
__
Sample Type:
__
High-throughput Compatibility:
__
For Use With (Application):
__
Starting Material Amount:
__
Isolation Technology: __
Elution Volume: __
Final Product Type: __
For Use With (Equipment): __
Shipping Condition: Dry Ice
Yield: __
Purification Time: __
Sample Type: __
High-throughput Compatibility: __
For Use With (Application): __
Starting Material Amount: __
Isolation Technology:
__
Elution Volume:
__
Final Product Type:
__
For Use With (Equipment):
__
Shipping Condition:
Dry Ice
Yield:
__
Purification Time:
__
Sample Type:
__
High-throughput Compatibility:
__
For Use With (Application):
__
Starting Material Amount:
__