Thermo Scientific™ GeneJET RNA Purification Kit

Description

• Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast
• The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions
• RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step
• The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses
• Highlights • Universal —can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria • Efficient —high yields of total RNA
• • Fast —15 minutes after lysis step • Pure —an A260/280 ratio > 1.9 • Convenient —spin columns are capped and assembled with collection tubes Applications Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including: • RT-PCR • RT-qPCR • Northern blotting • Nuclease protection assay Includes Proteinase K Lysis Buffer, Wash Buffer 1 (concentrated), Wash Buffer 2 (concentrated), Nuclease-free Water, GeneJET RNA Purification Columns (pre-assembled with Collection Tubes), Collection Tubes (2 mL), Collection Tubes (1.5 mL), Detailed Protocol