Thermo Scientific™ GeneJET Plant RNA Purification Kit

Description

• Description GeneJET Plant RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from wide variety of plant species and tissue types
• The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation
• The standard procedure takes less than 20 minutes following cell lysis
• Purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting and other RNA-based analysis
• RNA yields vary between different species, tissues, and age of tissue sample
• See Table 1 for typical total RNA yields from various sources
• Thermo Scientific GeneJET Plant RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from wide variety of plant species and tissue types
• The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation
• The standard procedure takes less than 20 minutes following cell lysis
• Purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting and other RNA-based analysis
• RNA yields vary between different species, tissues, and age of tissue sample
• See Table 1 for typical total RNA yields from various sources
• Highlights • Fast —procedure completed in 20 min following cell lysis • Convinient —protocol does not include clogging-prone lysate filtration step • High yield —up to 65 μg of high purity and high integrity RNA from 50 mg plant sample • Compatible with wide variety of samples (leaves, roots, sprouts and other parts of various plants) Applications Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including: • RT-PCR • RT-qPCR • Northern blotting • Nuclease protection assay