AM1924
Invitrogen™ RiboPure™ RNA Purification Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1924-Each-of-1 | In Stock | ₹ 54,587.28 |
AM1924 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 54,587.28
GST (18%): ₹ 9,825.71
Total Price: ₹ 64,412.99
Content And Storage
• 50 mL TRI Reagent; store at 4°C• 50 Filter Cartridges; room temperature• 100 Collection Tubes (2 mL); room temperature• 60 mL Wash Solution (concentrated); room temperature• 11 mL Elution Buffer; room temperature
Purification Time
30 min. (After sample homogenization)
Sample Type
Cells, Tissue
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Quantity
50 Preps
Starting Material Amount
Cells: 0.1–20 x 106Tissue: 5–100 mg
Isolation Technology
Spin Column (Glass Fiber Filter)
Elution Volume
100 μL
Final Product Type
Total RNA
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition
Room Temperature
Yield
Up to 500 μg
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Description
- The Ambion™ RiboPure™ Kit is designed for rapid purification of high-quality RNA from tissue samples or cultured cells
- The kit combines TRI Reagent™ with glass fiber filter purification to yield exceptionally pure RNA, free of residual proteins and lipids
- The kit includes sufficient reagents for 50 purifications from 1–100 mg of tissue or 0.1–20 x 10 6 cultured cells each
- • Yields the highest purity RNA attainable • Ideal for the most stringent downstream applications • Handles problematic samples with ease • Fast (less than 30 minutes) and easy procedure RNA isolated using the RiboPure™ Kit is suitable for use in all common downstream applications, including cDNA synthesis, real-time and end-point RT-PCR, microarray analysis, northern blots, and RNase protection assays
- The RiboPure™ protocol is especially suited for processing samples that are difficult to disrupt, or samples that have large amounts of ribonuclease or other contaminants
- Samples are first homogenized in TRI Reagent™, a monophasic solution containing phenol and guanidine thiocyanate, to rapidly lyse cells and inactivate nucleases
- Addition of bromochloropropane (BCP) results in separation of the homogenate into aqueous and organic phases
- RNA partitions to the aqueous phase, while DNA and protein remain in the interphase and organic phase
- The aqueous phase is removed and passed through a glass fiber filter that binds the RNA
- The filter is washed, and the RNA is eluted
- The procedure is compatible with tissues that have been stored in Ambion's RNA later ™
- Total RNA yield is typically 100–500 μg per 100 mg of tissue, depending on the type of tissue
- Order Info Shipping Condition: Room temperature
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Content And Storage: • 50 mL TRI Reagent; store at 4°C• 50 Filter Cartridges; room temperature• 100 Collection Tubes (2 mL); room temperature• 60 mL Wash Solution (concentrated); room temperature• 11 mL Elution Buffer; room temperature
Purification Time: 30 min. (After sample homogenization)
Sample Type: Cells, Tissue
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 50 Preps
Starting Material Amount: Cells: 0.1–20 x 106Tissue: 5–100 mg
Isolation Technology: Spin Column (Glass Fiber Filter)
Elution Volume: 100 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition: Room Temperature
Yield: Up to 500 μg
Content And Storage:
• 50 mL TRI Reagent; store at 4°C• 50 Filter Cartridges; room temperature• 100 Collection Tubes (2 mL); room temperature• 60 mL Wash Solution (concentrated); room temperature• 11 mL Elution Buffer; room temperature
Purification Time:
30 min. (After sample homogenization)
Sample Type:
Cells, Tissue
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
50 Preps
Starting Material Amount:
Cells: 0.1–20 x 106Tissue: 5–100 mg
Isolation Technology:
Spin Column (Glass Fiber Filter)
Elution Volume:
100 μL
Final Product Type:
Total RNA
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition:
Room Temperature
Yield:
Up to 500 μg
Content And Storage: • 50 1.5-mL Screw Cap Tubes; store at room temperature• 50 Filter Cartridges; room temperature• 100 Collection Tubes (2 mL); room temperature• 24 mL Lysis Buffer; 4°C• 2.4 mL 10% SDS; 4°C• 24 mL Phenol:Chloroform:IAA; 4°C• 95 mL Binding Buffer; 4°C• 35 mL Wash Solution 1; 4°C• 50 mL Wash Solution 2/3 (concentrated); 4°C• 5 mL Elution Solution; 4°C• 80 g Zirconia Beads; -20°C• 550 μL 10X DNase 1 Buffer; -20°C• 200 μL DNase 1; -20°C• 550 μL DNase Inactivation Reagent; -20°C
Purification Time: 1.5 hr. (Including DNase treatment)
Sample Type: Yeast
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 50 Preps
Starting Material Amount: Up to 108cells
Isolation Technology: Spin Column (Glass Fiber Filter)
Elution Volume: 50–100 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition: Box 1: Dry IceBox 2: Room Temperature
Yield: Up to 300 μg
Content And Storage:
• 50 1.5-mL Screw Cap Tubes; store at room temperature• 50 Filter Cartridges; room temperature• 100 Collection Tubes (2 mL); room temperature• 24 mL Lysis Buffer; 4°C• 2.4 mL 10% SDS; 4°C• 24 mL Phenol:Chloroform:IAA; 4°C• 95 mL Binding Buffer; 4°C• 35 mL Wash Solution 1; 4°C• 50 mL Wash Solution 2/3 (concentrated); 4°C• 5 mL Elution Solution; 4°C• 80 g Zirconia Beads; -20°C• 550 μL 10X DNase 1 Buffer; -20°C• 200 μL DNase 1; -20°C• 550 μL DNase Inactivation Reagent; -20°C
Purification Time:
1.5 hr. (Including DNase treatment)
Sample Type:
Yeast
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
50 Preps
Starting Material Amount:
Up to 108cells
Isolation Technology:
Spin Column (Glass Fiber Filter)
Elution Volume:
50–100 μL
Final Product Type:
Total RNA
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition:
Box 1: Dry IceBox 2: Room Temperature
Yield:
Up to 300 μg
Content And Storage: • 25 mL Elution Solution (4°C)• 40 mL Lysis Solution (4°C)• 25 mL Acid-Phenol:Chloroform (4°C)• 5 mL Sodium Acetate Solution (4°C)• 35 mL Wash Solution 1 (room temperature/4°C)• 70 mL Wash 2/3 Concentrate (room temperature/4°C)• 100 mL RNAlater™ Solution (room temperature)• 40 Filter Cartridges (room temperature)• 80 Collecion Tubes (room temperature)• 100 μL DNase I, 8 U/μL (-20°C, frost-free)• 850 μL 20X DNase Buffer (-20°C)• 1.2 mL DNase Inactivation Reagent (-20°C)• 1 mL Formaldehyde Load Dye (-20°C)This kit contains enough reagents for 40 RNA isolation from 300-500 μL whole blood.
Purification Time: 30 min.
Sample Type: Blood
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 40 preps
Starting Material Amount: Up to 0.5 ml whole blood
Isolation Technology: Organic Extraction, Spin Column
Elution Volume: __
Final Product Type: Total RNA
For Use With (Application): Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Shipping Condition: __
Yield: 4 μg
Content And Storage:
• 25 mL Elution Solution (4°C)• 40 mL Lysis Solution (4°C)• 25 mL Acid-Phenol:Chloroform (4°C)• 5 mL Sodium Acetate Solution (4°C)• 35 mL Wash Solution 1 (room temperature/4°C)• 70 mL Wash 2/3 Concentrate (room temperature/4°C)• 100 mL RNAlater™ Solution (room temperature)• 40 Filter Cartridges (room temperature)• 80 Collecion Tubes (room temperature)• 100 μL DNase I, 8 U/μL (-20°C, frost-free)• 850 μL 20X DNase Buffer (-20°C)• 1.2 mL DNase Inactivation Reagent (-20°C)• 1 mL Formaldehyde Load Dye (-20°C)This kit contains enough reagents for 40 RNA isolation from 300-500 μL whole blood.
Purification Time:
30 min.
Sample Type:
Blood
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
40 preps
Starting Material Amount:
Up to 0.5 ml whole blood
Isolation Technology:
Organic Extraction, Spin Column
Elution Volume:
__
Final Product Type:
Total RNA
For Use With (Application):
Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Shipping Condition:
__
Yield:
4 μg
Content And Storage: • 50 Filter Cartridges with Collection Tubes; store at room temperature• 50 Elution Tubes (1.5 mL); room temperature• 15 mL Wash Solution 1 (concentrated); room temperature• 28 mL Wash Solution 2/3 (concentrated); 4°C• 25 mL Lysis Solution; 4°C• 200 μL LCM Additive; -20°C• 55 μL DNase; -20°C• 600 μL 10X DNase I Buffer; -20°C• 150 μL DNase Inactivation Reagent; -20°C• 5 mL Elution Solution; room temperature
Purification Time: 15 min. (After sample homogenization)
Sample Type: Cells, Tissue, Laser Capture Microdissection (LCM)
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 50 Preps
Starting Material Amount: Cells: ≤105Tissue: ≤10 mg
Isolation Technology: Silica Spin Column
Elution Volume: 10–20 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition: Box 1: Dry IceBox 2: Room Temperature
Yield: __
Content And Storage:
• 50 Filter Cartridges with Collection Tubes; store at room temperature• 50 Elution Tubes (1.5 mL); room temperature• 15 mL Wash Solution 1 (concentrated); room temperature• 28 mL Wash Solution 2/3 (concentrated); 4°C• 25 mL Lysis Solution; 4°C• 200 μL LCM Additive; -20°C• 55 μL DNase; -20°C• 600 μL 10X DNase I Buffer; -20°C• 150 μL DNase Inactivation Reagent; -20°C• 5 mL Elution Solution; room temperature
Purification Time:
15 min. (After sample homogenization)
Sample Type:
Cells, Tissue, Laser Capture Microdissection (LCM)
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
50 Preps
Starting Material Amount:
Cells: ≤105Tissue: ≤10 mg
Isolation Technology:
Silica Spin Column
Elution Volume:
10–20 μL
Final Product Type:
Total RNA
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition:
Box 1: Dry IceBox 2: Room Temperature
Yield:
__