AM1926
Invitrogen™ RiboPure™ RNA Purification Kit, yeast
Manufacturer: Invitrogen™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1926-Each-of-1 | In Stock | ₹ 57,960.00 |
AM1926 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 57,960.00
GST (18%): ₹ 10,432.80
Total Price: ₹ 68,392.80
Content And Storage
• 50 1.5-mL Screw Cap Tubes; store at room temperature• 50 Filter Cartridges; room temperature• 100 Collection Tubes (2 mL); room temperature• 24 mL Lysis Buffer; 4°C• 2.4 mL 10% SDS; 4°C• 24 mL Phenol:Chloroform:IAA; 4°C• 95 mL Binding Buffer; 4°C• 35 mL Wash Solution 1; 4°C• 50 mL Wash Solution 2/3 (concentrated); 4°C• 5 mL Elution Solution; 4°C• 80 g Zirconia Beads; -20°C• 550 μL 10X DNase 1 Buffer; -20°C• 200 μL DNase 1; -20°C• 550 μL DNase Inactivation Reagent; -20°C
Purification Time
1.5 hr. (Including DNase treatment)
Sample Type
Yeast
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Quantity
50 Preps
Starting Material Amount
Up to 108cells
Isolation Technology
Spin Column (Glass Fiber Filter)
Elution Volume
50–100 μL
Final Product Type
Total RNA
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition
Box 1: Dry IceBox 2: Room Temperature
Yield
Up to 300 μg
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Description
- Description For the isolation of extremely high-quality RNA from yeast without the use of enzymatic pretreatments that may alter gene expression profiles
- Each Invitrogen kit includes sufficient reagents for 50 purifications each from 10 7 to 10 8 yeast cells
- It has traditionally been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells
- Many protocols use enzymatic pretreatments that may affect gene expression patterns
- The RiboPure-Yeast RNA Isolation Kit uses no enzymatic pretreatments and thus does not alter expression profiles
- It combines a simple and efficient glass bead/one step phenol–mediated disruption using Invitrogen's Vortex Bead Beater Adapter followed by a glass fiber filter– based RNA purification step
- After DNase treatment with the included DNA- free ™ Reagents, the resulting RNA is exceptionally pure containing little to no genomic DNA (less than 0.05%)
- The kit has been used extensively at Invitrogen to isolate high-quality RNA from Saccharomyces cerevisiae , Schizosaccharomyces pombe , Pichia pastoris , and Candida albicans
- The kit provides the highest yield of any method tested from each of these species
- Highest yields of total RNA available No enzymatic pretreatment required Includes DNA- free ™ Reagents Not high-throughput compatible (Manual) Applications : DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Yeast
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Content And Storage: • 50 1.5-mL Screw Cap Tubes; store at room temperature• 50 Filter Cartridges; room temperature• 100 Collection Tubes (2 mL); room temperature• 24 mL Lysis Buffer; 4°C• 2.4 mL 10% SDS; 4°C• 24 mL Phenol:Chloroform:IAA; 4°C• 95 mL Binding Buffer; 4°C• 35 mL Wash Solution 1; 4°C• 50 mL Wash Solution 2/3 (concentrated); 4°C• 5 mL Elution Solution; 4°C• 80 g Zirconia Beads; -20°C• 550 μL 10X DNase 1 Buffer; -20°C• 200 μL DNase 1; -20°C• 550 μL DNase Inactivation Reagent; -20°C
Purification Time: 1.5 hr. (Including DNase treatment)
Sample Type: Yeast
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 50 Preps
Starting Material Amount: Up to 108cells
Isolation Technology: Spin Column (Glass Fiber Filter)
Elution Volume: 50–100 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition: Box 1: Dry IceBox 2: Room Temperature
Yield: Up to 300 μg
Content And Storage:
• 50 1.5-mL Screw Cap Tubes; store at room temperature• 50 Filter Cartridges; room temperature• 100 Collection Tubes (2 mL); room temperature• 24 mL Lysis Buffer; 4°C• 2.4 mL 10% SDS; 4°C• 24 mL Phenol:Chloroform:IAA; 4°C• 95 mL Binding Buffer; 4°C• 35 mL Wash Solution 1; 4°C• 50 mL Wash Solution 2/3 (concentrated); 4°C• 5 mL Elution Solution; 4°C• 80 g Zirconia Beads; -20°C• 550 μL 10X DNase 1 Buffer; -20°C• 200 μL DNase 1; -20°C• 550 μL DNase Inactivation Reagent; -20°C
Purification Time:
1.5 hr. (Including DNase treatment)
Sample Type:
Yeast
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
50 Preps
Starting Material Amount:
Up to 108cells
Isolation Technology:
Spin Column (Glass Fiber Filter)
Elution Volume:
50–100 μL
Final Product Type:
Total RNA
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition:
Box 1: Dry IceBox 2: Room Temperature
Yield:
Up to 300 μg
Content And Storage: • 25 mL Elution Solution (4°C)• 40 mL Lysis Solution (4°C)• 25 mL Acid-Phenol:Chloroform (4°C)• 5 mL Sodium Acetate Solution (4°C)• 35 mL Wash Solution 1 (room temperature/4°C)• 70 mL Wash 2/3 Concentrate (room temperature/4°C)• 100 mL RNAlater™ Solution (room temperature)• 40 Filter Cartridges (room temperature)• 80 Collecion Tubes (room temperature)• 100 μL DNase I, 8 U/μL (-20°C, frost-free)• 850 μL 20X DNase Buffer (-20°C)• 1.2 mL DNase Inactivation Reagent (-20°C)• 1 mL Formaldehyde Load Dye (-20°C)This kit contains enough reagents for 40 RNA isolation from 300-500 μL whole blood.
Purification Time: 30 min.
Sample Type: Blood
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 40 preps
Starting Material Amount: Up to 0.5 ml whole blood
Isolation Technology: Organic Extraction, Spin Column
Elution Volume: __
Final Product Type: Total RNA
For Use With (Application): Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Shipping Condition: __
Yield: 4 μg
Content And Storage:
• 25 mL Elution Solution (4°C)• 40 mL Lysis Solution (4°C)• 25 mL Acid-Phenol:Chloroform (4°C)• 5 mL Sodium Acetate Solution (4°C)• 35 mL Wash Solution 1 (room temperature/4°C)• 70 mL Wash 2/3 Concentrate (room temperature/4°C)• 100 mL RNAlater™ Solution (room temperature)• 40 Filter Cartridges (room temperature)• 80 Collecion Tubes (room temperature)• 100 μL DNase I, 8 U/μL (-20°C, frost-free)• 850 μL 20X DNase Buffer (-20°C)• 1.2 mL DNase Inactivation Reagent (-20°C)• 1 mL Formaldehyde Load Dye (-20°C)This kit contains enough reagents for 40 RNA isolation from 300-500 μL whole blood.
Purification Time:
30 min.
Sample Type:
Blood
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
40 preps
Starting Material Amount:
Up to 0.5 ml whole blood
Isolation Technology:
Organic Extraction, Spin Column
Elution Volume:
__
Final Product Type:
Total RNA
For Use With (Application):
Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Shipping Condition:
__
Yield:
4 μg
Content And Storage: • 50 Filter Cartridges with Collection Tubes; store at room temperature• 50 Elution Tubes (1.5 mL); room temperature• 15 mL Wash Solution 1 (concentrated); room temperature• 28 mL Wash Solution 2/3 (concentrated); 4°C• 25 mL Lysis Solution; 4°C• 200 μL LCM Additive; -20°C• 55 μL DNase; -20°C• 600 μL 10X DNase I Buffer; -20°C• 150 μL DNase Inactivation Reagent; -20°C• 5 mL Elution Solution; room temperature
Purification Time: 15 min. (After sample homogenization)
Sample Type: Cells, Tissue, Laser Capture Microdissection (LCM)
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 50 Preps
Starting Material Amount: Cells: ≤105Tissue: ≤10 mg
Isolation Technology: Silica Spin Column
Elution Volume: 10–20 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition: Box 1: Dry IceBox 2: Room Temperature
Yield: __
Content And Storage:
• 50 Filter Cartridges with Collection Tubes; store at room temperature• 50 Elution Tubes (1.5 mL); room temperature• 15 mL Wash Solution 1 (concentrated); room temperature• 28 mL Wash Solution 2/3 (concentrated); 4°C• 25 mL Lysis Solution; 4°C• 200 μL LCM Additive; -20°C• 55 μL DNase; -20°C• 600 μL 10X DNase I Buffer; -20°C• 150 μL DNase Inactivation Reagent; -20°C• 5 mL Elution Solution; room temperature
Purification Time:
15 min. (After sample homogenization)
Sample Type:
Cells, Tissue, Laser Capture Microdissection (LCM)
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
50 Preps
Starting Material Amount:
Cells: ≤105Tissue: ≤10 mg
Isolation Technology:
Silica Spin Column
Elution Volume:
10–20 μL
Final Product Type:
Total RNA
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition:
Box 1: Dry IceBox 2: Room Temperature
Yield:
__
Content And Storage: Processing Plate & Lid, Binding Solution Concentrate, Wash Solution 1 Concentrate, Wash Solution 2 Concentrate, Elution Buffer are all stored at room temperature. RNA Binding Beads are stored at 4°C and the Lysis ⁄Binding Enhancer is stored at –20°C.
Purification Time: 45 min.
Sample Type: Cell-free Biological Fluids, Liquid Samples (e.g. Serum), Plasma, Serum, Viral Samples
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 50 preps
Starting Material Amount: Up to 400 μl of sample
Isolation Technology: Magnetic Bead
Elution Volume: __
Final Product Type: Viral DNA⁄RNA
For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Shipping Condition: __
Yield: 10 ng
Content And Storage:
Processing Plate & Lid, Binding Solution Concentrate, Wash Solution 1 Concentrate, Wash Solution 2 Concentrate, Elution Buffer are all stored at room temperature. RNA Binding Beads are stored at 4°C and the Lysis ⁄Binding Enhancer is stored at –20°C.
Purification Time:
45 min.
Sample Type:
Cell-free Biological Fluids, Liquid Samples (e.g. Serum), Plasma, Serum, Viral Samples
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
50 preps
Starting Material Amount:
Up to 400 μl of sample
Isolation Technology:
Magnetic Bead
Elution Volume:
__
Final Product Type:
Viral DNA⁄RNA
For Use With (Application):
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Shipping Condition:
__
Yield:
10 ng