AM1914
Invitrogen™ RNAqueous™-4PCR Total RNA Isolation Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1914-Each-of-1 | In Stock | ₹ 27,968.00 |
AM1914 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 27,968.00
GST (18%): ₹ 5,034.24
Total Price: ₹ 33,002.24
Content And Storage
• 600 μL 10X DNase 1 Buffer; store at -20°C• 45 μL DNase 1; -20°C• 1 mL Formaldehye Load Dye; -20°C• 100 μL Linear Acrylamide; -20°C• 50 μL Positive Control Primers; -20°C• 1 mL 5M Ammonium Acetate; -20°C• 35 mL Water; 4°C• 600 μL DNase Inactivation Reagent; 4°C• 30 mL Lysis/Binding Solution; 4°C• 25 mL Wash Solution #1; 4°C• 35 mL Wash Solution 2/3 (concentrated); 4°C• 3 Pestles; room temperature• 30 Filter Cartridges; room temperature• 60 Collection Tubes; room temperature• 25 mL Elution Solution; room temperature
Purification Time
10 min. (After sample homogenization)
Sample Type
Tissue, Cells
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Quantity
30 Preps
Starting Material Amount
Tissue: ≤75 mgCells: ≤107
Isolation Technology
Silica Spin Column
Elution Volume
50–120 μL
Final Product Type
Total RNA
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition
Box 1: Dry IceBox 2: Room Temperature
Yield
≤10 μg per 1 mg tissue≤1 μg per 1 x 105cells
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Description
- Description The RNAqueous-4PCR Kit is used for phenol-free total RNA isolation from small tissue samples (0.5 to 75 mg) or from cells (∽100 cells to ∽1 x 10 7 cells), using a guanidinium-based lysis/denaturant and glass fiber filter separation technology
- DNA- free ™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR
- DNA-free RNA for RT-PCR Rapid, phenol-free total RNA isolation Novel reagent for rapid and safe removal of DNase and divalent cations Using the RNAqueous-4PCR Kit The RNAqueous-4PCR Kit is designed for isolatation of RNA for applications such as RT-PCR, that would benefit from its robust genomic DNA removal capabilities
- When used in conjunction with RNA later ™ Tissue Collection: RNA Stabilization Solution, most tissue samples can be stored at 4°C and then easily disrupted in standard microfuge tubes using the pestles provided in the kit
- No need for snap-freezing in liquid nitrogen or grinding in a mortar and pestle
- Not high-throughput compatible (Manual) Applications: DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues
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Content And Storage: • 600 μL 10X DNase 1 Buffer; store at -20°C• 45 μL DNase 1; -20°C• 1 mL Formaldehye Load Dye; -20°C• 100 μL Linear Acrylamide; -20°C• 50 μL Positive Control Primers; -20°C• 1 mL 5M Ammonium Acetate; -20°C• 35 mL Water; 4°C• 600 μL DNase Inactivation Reagent; 4°C• 30 mL Lysis/Binding Solution; 4°C• 25 mL Wash Solution #1; 4°C• 35 mL Wash Solution 2/3 (concentrated); 4°C• 3 Pestles; room temperature• 30 Filter Cartridges; room temperature• 60 Collection Tubes; room temperature• 25 mL Elution Solution; room temperature
Purification Time: 10 min. (After sample homogenization)
Sample Type: Tissue, Cells
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 30 Preps
Starting Material Amount: Tissue: ≤75 mgCells: ≤107
Isolation Technology: Silica Spin Column
Elution Volume: 50–120 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition: Box 1: Dry IceBox 2: Room Temperature
Yield: ≤10 μg per 1 mg tissue≤1 μg per 1 x 105cells
Content And Storage:
• 600 μL 10X DNase 1 Buffer; store at -20°C• 45 μL DNase 1; -20°C• 1 mL Formaldehye Load Dye; -20°C• 100 μL Linear Acrylamide; -20°C• 50 μL Positive Control Primers; -20°C• 1 mL 5M Ammonium Acetate; -20°C• 35 mL Water; 4°C• 600 μL DNase Inactivation Reagent; 4°C• 30 mL Lysis/Binding Solution; 4°C• 25 mL Wash Solution #1; 4°C• 35 mL Wash Solution 2/3 (concentrated); 4°C• 3 Pestles; room temperature• 30 Filter Cartridges; room temperature• 60 Collection Tubes; room temperature• 25 mL Elution Solution; room temperature
Purification Time:
10 min. (After sample homogenization)
Sample Type:
Tissue, Cells
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
30 Preps
Starting Material Amount:
Tissue: ≤75 mgCells: ≤107
Isolation Technology:
Silica Spin Column
Elution Volume:
50–120 μL
Final Product Type:
Total RNA
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition:
Box 1: Dry IceBox 2: Room Temperature
Yield:
≤10 μg per 1 mg tissue≤1 μg per 1 x 105cells
Content And Storage: • 50 mL Nuclease-free Water; store at room temperature• 50 mL Elution Solution; room temperature• 2 Filter Plates; room temperature• 2 Collection Plates; room temperature• 2 Adhesive Plate Sealers; room temperature• 2 Plate Lids; room temperature• 50 mL Lysis/Binding Solution; 4°C• 175 mL Wash Solution (concentrated); 4°C• 53 mL Rebinding Mix; 4°C• 12 mL DNase I Buffer; -20°C• 1.25 mL DNase I; -20°C
Purification Time: __
Sample Type: Cells, Tissue
High-throughput Compatibility: High-throughput Compatible
Quantity: 192 Preps
Starting Material Amount: Cells: ≤106Tissue: ≤1.5 mg
Isolation Technology: Silica Plate
Elution Volume: ≥50 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition: Box 1: Dry IceBox 2: Room Temperature
Yield: __
Content And Storage:
• 50 mL Nuclease-free Water; store at room temperature• 50 mL Elution Solution; room temperature• 2 Filter Plates; room temperature• 2 Collection Plates; room temperature• 2 Adhesive Plate Sealers; room temperature• 2 Plate Lids; room temperature• 50 mL Lysis/Binding Solution; 4°C• 175 mL Wash Solution (concentrated); 4°C• 53 mL Rebinding Mix; 4°C• 12 mL DNase I Buffer; -20°C• 1.25 mL DNase I; -20°C
Purification Time:
__
Sample Type:
Cells, Tissue
High-throughput Compatibility:
High-throughput Compatible
Quantity:
192 Preps
Starting Material Amount:
Cells: ≤106Tissue: ≤1.5 mg
Isolation Technology:
Silica Plate
Elution Volume:
≥50 μL
Final Product Type:
Total RNA
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition:
Box 1: Dry IceBox 2: Room Temperature
Yield:
__
Content And Storage: • 30 mL Cell Disruption Buffer; 4°C• 25 mL Cell Fractionation Buffer; 4°C• 30 mL 2X Lysis/Binding Solution; 4°C• 40 mL Wash Solution 1; 4°C• 80 mL Wash Solution 2/3 Concentrate; 4°C• 4 mL Lithium Chloride Precipitation Solution; 4°C• 50 Filter Cartridges; room temperature• 100 Collection Tubes; room temperature• 10 mL Elution Solution; room temperature
Purification Time: 20 min.
Sample Type: Cells, Tissue
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 50 Preps
Starting Material Amount: Cells: ≤107cellsTissue: ≤75 mg tissue
Isolation Technology: Spin Column (Glass Fiber Filter)
Elution Volume: 50 to 200 μL
Final Product Type: Total RNA and Protein
For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Western blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, enzymatic assays, immunoprecipitation, gel shift assays, 2D gel electrophoresis
Shipping Condition: Room Temperature
Yield: ≤1 μg per 105cells≤10 μg per 1 mg tissue
Content And Storage:
• 30 mL Cell Disruption Buffer; 4°C• 25 mL Cell Fractionation Buffer; 4°C• 30 mL 2X Lysis/Binding Solution; 4°C• 40 mL Wash Solution 1; 4°C• 80 mL Wash Solution 2/3 Concentrate; 4°C• 4 mL Lithium Chloride Precipitation Solution; 4°C• 50 Filter Cartridges; room temperature• 100 Collection Tubes; room temperature• 10 mL Elution Solution; room temperature
Purification Time:
20 min.
Sample Type:
Cells, Tissue
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
50 Preps
Starting Material Amount:
Cells: ≤107cellsTissue: ≤75 mg tissue
Isolation Technology:
Spin Column (Glass Fiber Filter)
Elution Volume:
50 to 200 μL
Final Product Type:
Total RNA and Protein
For Use With (Application):
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Western blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, enzymatic assays, immunoprecipitation, gel shift assays, 2D gel electrophoresis
Shipping Condition:
Room Temperature
Yield:
≤1 μg per 105cells≤10 μg per 1 mg tissue
Content And Storage: Store Nuclease-free Water, Microfuge Tubes and Microfuge tubes with Spin Columns at room temperature. Store 2X Binding Solution, Wash Solution 1, Wash Solution 2 and Oligo(dT) Cellulose at 4°C. Store RNA Storage Solution, 5M Ammonium Acetate and Glycogen(5 mg/mL) at -20°C.
Purification Time: __
Sample Type: Total RNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: __
Starting Material Amount: __
Isolation Technology: Magnetic Bead, Oligo dT Affinity
Elution Volume: __
Final Product Type: mRNA
For Use With (Application): Microarray Analysis, Reverse Transcriptase PCR (RT-PCR), Microinjection, Nucleic Acid Labeling, In Vitro Translation, cDNA Library Construction, Northern Blotting, Nuclease Protection Assays
Shipping Condition: Room Temperature
Yield: __
Content And Storage:
Store Nuclease-free Water, Microfuge Tubes and Microfuge tubes with Spin Columns at room temperature. Store 2X Binding Solution, Wash Solution 1, Wash Solution 2 and Oligo(dT) Cellulose at 4°C. Store RNA Storage Solution, 5M Ammonium Acetate and Glycogen(5 mg/mL) at -20°C.
Purification Time:
__
Sample Type:
Total RNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
__
Starting Material Amount:
__
Isolation Technology:
Magnetic Bead, Oligo dT Affinity
Elution Volume:
__
Final Product Type:
mRNA
For Use With (Application):
Microarray Analysis, Reverse Transcriptase PCR (RT-PCR), Microinjection, Nucleic Acid Labeling, In Vitro Translation, cDNA Library Construction, Northern Blotting, Nuclease Protection Assays
Shipping Condition:
Room Temperature
Yield:
__