Invitrogen™ RNAqueous™-4PCR Total RNA Isolation Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1914-Each-of-1 | In Stock | ₹ 26,010.24 |
AM1914 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 26,010.24
GST (18%): ₹ 4,681.843
Total Price: ₹ 30,692.083
Content And Storage
• 600 μL 10X DNase 1 Buffer; store at -20°C• 45 μL DNase 1; -20°C• 1 mL Formaldehye Load Dye; -20°C• 100 μL Linear Acrylamide; -20°C• 50 μL Positive Control Primers; -20°C• 1 mL 5M Ammonium Acetate; -20°C• 35 mL Water; 4°C• 600 μL DNase Inactivation Reagent; 4°C• 30 mL Lysis/Binding Solution; 4°C• 25 mL Wash Solution #1; 4°C• 35 mL Wash Solution 2/3 (concentrated); 4°C• 3 Pestles; room temperature• 30 Filter Cartridges; room temperature• 60 Collection Tubes; room temperature• 25 mL Elution Solution; room temperature
Purification Time
10 min. (After sample homogenization)
Sample Type
Tissue, Cells
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Quantity
30 Preps
Starting Material Amount
Tissue: ≤75 mgCells: ≤107
Isolation Technology
Silica Spin Column
Elution Volume
50–120 μL
Final Product Type
Total RNA
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition
Box 1: Dry IceBox 2: Room Temperature
Yield
≤10 μg per 1 mg tissue≤1 μg per 1 x 105cells
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Description
- The RNAqueous-4PCR Kit is used for phenol-free total RNA isolation from small tissue samples (0.5 to 75 mg) or from cells (∽100 cells to ∽1 x 10 7 cells), using a guanidinium-based lysis/denaturant and glass fiber filter separation technology
- DNA- free ™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR
- DNA-free RNA for RT-PCR Rapid, phenol-free total RNA isolation Novel reagent for rapid and safe removal of DNase and divalent cations Using the RNAqueous-4PCR Kit The RNAqueous-4PCR Kit is designed for isolatation of RNA for applications such as RT-PCR, that would benefit from its robust genomic DNA removal capabilities
- When used in conjunction with RNA later ™ Tissue Collection: RNA Stabilization Solution, most tissue samples can be stored at 4°C and then easily disrupted in standard microfuge tubes using the pestles provided in the kit
- No need for snap-freezing in liquid nitrogen or grinding in a mortar and pestle
- Not high-throughput compatible (Manual) Applications: DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues
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Content And Storage: • 600 μL 10X DNase 1 Buffer; store at -20°C• 45 μL DNase 1; -20°C• 1 mL Formaldehye Load Dye; -20°C• 100 μL Linear Acrylamide; -20°C• 50 μL Positive Control Primers; -20°C• 1 mL 5M Ammonium Acetate; -20°C• 35 mL Water; 4°C• 600 μL DNase Inactivation Reagent; 4°C• 30 mL Lysis/Binding Solution; 4°C• 25 mL Wash Solution #1; 4°C• 35 mL Wash Solution 2/3 (concentrated); 4°C• 3 Pestles; room temperature• 30 Filter Cartridges; room temperature• 60 Collection Tubes; room temperature• 25 mL Elution Solution; room temperature
Purification Time: 10 min. (After sample homogenization)
Sample Type: Tissue, Cells
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 30 Preps
Starting Material Amount: Tissue: ≤75 mgCells: ≤107
Isolation Technology: Silica Spin Column
Elution Volume: 50–120 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition: Box 1: Dry IceBox 2: Room Temperature
Yield: ≤10 μg per 1 mg tissue≤1 μg per 1 x 105cells
Content And Storage: __
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Purification Time: __
Sample Type: __
High-throughput Compatibility: __
Quantity: __
Starting Material Amount: __
Isolation Technology: __
Elution Volume: __
Final Product Type: __
For Use With (Application): __
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