AM1912
Invitrogen™ RNAqueous™ Total RNA Isolation Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1912-Each-of-1 | In Stock | ₹ 39,192.00 |
AM1912 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 39,192.00
GST (18%): ₹ 7,054.56
Total Price: ₹ 46,246.56
Content And Storage
• 6 mL Formaldehyde Load Dye; store at -20°C• 60 mL Lysis/Binding Solution; 4°C• 60 mL Water; 4°C• 4 mL Lithium Chloride Precipitation Solution; 4°C• 40 mL Wash Solution #1; 4°C• 80 mL Wash Solution #2/3 (concentrated); 4°C• 50 Filter Cartridges; room temperature• 100 Collection Tubes; room temperature• 10 mL Elution Solution; room temperature
Elution Volume
50–120 μL
Final Product Type
Total RNA
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition
Room Temperature
Isolation Technology
Spin Column (Glass Fiber Filter)
Sample Type
Bacteria, Cells, Plant, Tissue, Yeast, Viruses, Enzymatic Reactions
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Quantity
50 Preps
Starting Material Amount
Bacteria: ≤109cellsCells: ≤107Tissue: ≤75 mgPlant: ≤75 mgYeast: ≤108cellsVirus: variesEnzymatic Reactions: varies
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Description
- Description The RNAqueous Kit is for phenol-free total RNA isolation from 0.1–0.5 g of tissue or from 10 7 to 10 8 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology
- Convenient syringe-based filtration Compatibility with vacuum manifolds when processing multiple samples How it works The RNAqueous Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter
- Washing and elution solutions are simply pushed through the filter using a standard syringe (not included)
- Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once
- Not high-throughput compatible (Manual) Applications: DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues
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Content And Storage: • 6 mL Formaldehyde Load Dye; store at -20°C• 60 mL Lysis/Binding Solution; 4°C• 60 mL Water; 4°C• 4 mL Lithium Chloride Precipitation Solution; 4°C• 40 mL Wash Solution #1; 4°C• 80 mL Wash Solution #2/3 (concentrated); 4°C• 50 Filter Cartridges; room temperature• 100 Collection Tubes; room temperature• 10 mL Elution Solution; room temperature
Elution Volume: 50–120 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition: Room Temperature
Isolation Technology: Spin Column (Glass Fiber Filter)
Sample Type: Bacteria, Cells, Plant, Tissue, Yeast, Viruses, Enzymatic Reactions
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 50 Preps
Starting Material Amount: Bacteria: ≤109cellsCells: ≤107Tissue: ≤75 mgPlant: ≤75 mgYeast: ≤108cellsVirus: variesEnzymatic Reactions: varies
Content And Storage:
• 6 mL Formaldehyde Load Dye; store at -20°C• 60 mL Lysis/Binding Solution; 4°C• 60 mL Water; 4°C• 4 mL Lithium Chloride Precipitation Solution; 4°C• 40 mL Wash Solution #1; 4°C• 80 mL Wash Solution #2/3 (concentrated); 4°C• 50 Filter Cartridges; room temperature• 100 Collection Tubes; room temperature• 10 mL Elution Solution; room temperature
Elution Volume:
50–120 μL
Final Product Type:
Total RNA
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition:
Room Temperature
Isolation Technology:
Spin Column (Glass Fiber Filter)
Sample Type:
Bacteria, Cells, Plant, Tissue, Yeast, Viruses, Enzymatic Reactions
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
50 Preps
Starting Material Amount:
Bacteria: ≤109cellsCells: ≤107Tissue: ≤75 mgPlant: ≤75 mgYeast: ≤108cellsVirus: variesEnzymatic Reactions: varies
Content And Storage: • 600 μL 10X DNase 1 Buffer; store at -20°C• 45 μL DNase 1; -20°C• 1 mL Formaldehye Load Dye; -20°C• 100 μL Linear Acrylamide; -20°C• 50 μL Positive Control Primers; -20°C• 1 mL 5M Ammonium Acetate; -20°C• 35 mL Water; 4°C• 600 μL DNase Inactivation Reagent; 4°C• 30 mL Lysis/Binding Solution; 4°C• 25 mL Wash Solution #1; 4°C• 35 mL Wash Solution 2/3 (concentrated); 4°C• 3 Pestles; room temperature• 30 Filter Cartridges; room temperature• 60 Collection Tubes; room temperature• 25 mL Elution Solution; room temperature
Elution Volume: 50–120 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition: Box 1: Dry IceBox 2: Room Temperature
Isolation Technology: Silica Spin Column
Sample Type: Tissue, Cells
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 30 Preps
Starting Material Amount: Tissue: ≤75 mgCells: ≤107
Content And Storage:
• 600 μL 10X DNase 1 Buffer; store at -20°C• 45 μL DNase 1; -20°C• 1 mL Formaldehye Load Dye; -20°C• 100 μL Linear Acrylamide; -20°C• 50 μL Positive Control Primers; -20°C• 1 mL 5M Ammonium Acetate; -20°C• 35 mL Water; 4°C• 600 μL DNase Inactivation Reagent; 4°C• 30 mL Lysis/Binding Solution; 4°C• 25 mL Wash Solution #1; 4°C• 35 mL Wash Solution 2/3 (concentrated); 4°C• 3 Pestles; room temperature• 30 Filter Cartridges; room temperature• 60 Collection Tubes; room temperature• 25 mL Elution Solution; room temperature
Elution Volume:
50–120 μL
Final Product Type:
Total RNA
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition:
Box 1: Dry IceBox 2: Room Temperature
Isolation Technology:
Silica Spin Column
Sample Type:
Tissue, Cells
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
30 Preps
Starting Material Amount:
Tissue: ≤75 mgCells: ≤107
Content And Storage: • 50 mL Nuclease-free Water; store at room temperature• 50 mL Elution Solution; room temperature• 2 Filter Plates; room temperature• 2 Collection Plates; room temperature• 2 Adhesive Plate Sealers; room temperature• 2 Plate Lids; room temperature• 50 mL Lysis/Binding Solution; 4°C• 175 mL Wash Solution (concentrated); 4°C• 53 mL Rebinding Mix; 4°C• 12 mL DNase I Buffer; -20°C• 1.25 mL DNase I; -20°C
Elution Volume: ≥50 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition: Box 1: Dry IceBox 2: Room Temperature
Isolation Technology: Silica Plate
Sample Type: Cells, Tissue
High-throughput Compatibility: High-throughput Compatible
Quantity: 192 Preps
Starting Material Amount: Cells: ≤106Tissue: ≤1.5 mg
Content And Storage:
• 50 mL Nuclease-free Water; store at room temperature• 50 mL Elution Solution; room temperature• 2 Filter Plates; room temperature• 2 Collection Plates; room temperature• 2 Adhesive Plate Sealers; room temperature• 2 Plate Lids; room temperature• 50 mL Lysis/Binding Solution; 4°C• 175 mL Wash Solution (concentrated); 4°C• 53 mL Rebinding Mix; 4°C• 12 mL DNase I Buffer; -20°C• 1.25 mL DNase I; -20°C
Elution Volume:
≥50 μL
Final Product Type:
Total RNA
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition:
Box 1: Dry IceBox 2: Room Temperature
Isolation Technology:
Silica Plate
Sample Type:
Cells, Tissue
High-throughput Compatibility:
High-throughput Compatible
Quantity:
192 Preps
Starting Material Amount:
Cells: ≤106Tissue: ≤1.5 mg
Content And Storage: • 30 mL Cell Disruption Buffer; 4°C• 25 mL Cell Fractionation Buffer; 4°C• 30 mL 2X Lysis/Binding Solution; 4°C• 40 mL Wash Solution 1; 4°C• 80 mL Wash Solution 2/3 Concentrate; 4°C• 4 mL Lithium Chloride Precipitation Solution; 4°C• 50 Filter Cartridges; room temperature• 100 Collection Tubes; room temperature• 10 mL Elution Solution; room temperature
Elution Volume: 50 to 200 μL
Final Product Type: Total RNA and Protein
For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Western blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, enzymatic assays, immunoprecipitation, gel shift assays, 2D gel electrophoresis
Shipping Condition: Room Temperature
Isolation Technology: Spin Column (Glass Fiber Filter)
Sample Type: Cells, Tissue
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 50 Preps
Starting Material Amount: Cells: ≤107cellsTissue: ≤75 mg tissue
Content And Storage:
• 30 mL Cell Disruption Buffer; 4°C• 25 mL Cell Fractionation Buffer; 4°C• 30 mL 2X Lysis/Binding Solution; 4°C• 40 mL Wash Solution 1; 4°C• 80 mL Wash Solution 2/3 Concentrate; 4°C• 4 mL Lithium Chloride Precipitation Solution; 4°C• 50 Filter Cartridges; room temperature• 100 Collection Tubes; room temperature• 10 mL Elution Solution; room temperature
Elution Volume:
50 to 200 μL
Final Product Type:
Total RNA and Protein
For Use With (Application):
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Western blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, enzymatic assays, immunoprecipitation, gel shift assays, 2D gel electrophoresis
Shipping Condition:
Room Temperature
Isolation Technology:
Spin Column (Glass Fiber Filter)
Sample Type:
Cells, Tissue
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
50 Preps
Starting Material Amount:
Cells: ≤107cellsTissue: ≤75 mg tissue