AM1931
Invitrogen™ RNAqueous™-Micro Total RNA Isolation Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1931-Each-of-1 | In Stock | ₹ 44,896.00 |
AM1931 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 44,896.00
GST (18%): ₹ 8,081.28
Total Price: ₹ 52,977.28
Content And Storage
• 50 Filter Cartridges with Collection Tubes; store at room temperature• 50 Elution Tubes (1.5 mL); room temperature• 15 mL Wash Solution 1 (concentrated); room temperature• 28 mL Wash Solution 2/3 (concentrated); 4°C• 25 mL Lysis Solution; 4°C• 200 μL LCM Additive; -20°C• 55 μL DNase; -20°C• 600 μL 10X DNase I Buffer; -20°C• 150 μL DNase Inactivation Reagent; -20°C• 5 mL Elution Solution; room temperature
Purification Time
15 min. (After sample homogenization)
Sample Type
Cells, Tissue, Laser Capture Microdissection (LCM)
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Quantity
50 Preps
Starting Material Amount
Cells: ≤105Tissue: ≤10 mg
Isolation Technology
Silica Spin Column
Elution Volume
10–20 μL
Final Product Type
Total RNA
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition
Box 1: Dry IceBox 2: Room Temperature
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Description
- Description The RNAqueous™-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e.g., from less than 500,000 cultured cells, from few as 10 laser capture microdissected cells, or from ≤10 mg of tissue) using a guanidinium-based lysis/denaturant and glass fiber filter separation technology
- DNA- free ™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR
- This kit provides: • Highly concentrated RNA—for increased sensitivity in downstream assays • Isolation of high-quality RNA from micro-scale samples • Reagents/protocol for RNA recovery from LCM samples How it works Samples are solubilized in a powerful chaotropic solution, and RNA is purified on a glass fiber filter cartridge specifically designed for very small samples
- A simple modification of the protocol allows recovery of both large and small RNA species including tRNAs, 5S rRNA, and microRNAs
- The RNA is eluted in a minimal volume of 20 μL
- Residual genomic DNA is efficiently removed using the DNA- free ™ system included in the kit
- The RNAqueous™-Micro Kit contains enough reagents for 50 RNA isolations of DNA-free RNA
- Not high-throughput compatible (Manual) Applications DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues
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Content And Storage: • 50 Filter Cartridges with Collection Tubes; store at room temperature• 50 Elution Tubes (1.5 mL); room temperature• 15 mL Wash Solution 1 (concentrated); room temperature• 28 mL Wash Solution 2/3 (concentrated); 4°C• 25 mL Lysis Solution; 4°C• 200 μL LCM Additive; -20°C• 55 μL DNase; -20°C• 600 μL 10X DNase I Buffer; -20°C• 150 μL DNase Inactivation Reagent; -20°C• 5 mL Elution Solution; room temperature
Purification Time: 15 min. (After sample homogenization)
Sample Type: Cells, Tissue, Laser Capture Microdissection (LCM)
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 50 Preps
Starting Material Amount: Cells: ≤105Tissue: ≤10 mg
Isolation Technology: Silica Spin Column
Elution Volume: 10–20 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition: Box 1: Dry IceBox 2: Room Temperature
Content And Storage:
• 50 Filter Cartridges with Collection Tubes; store at room temperature• 50 Elution Tubes (1.5 mL); room temperature• 15 mL Wash Solution 1 (concentrated); room temperature• 28 mL Wash Solution 2/3 (concentrated); 4°C• 25 mL Lysis Solution; 4°C• 200 μL LCM Additive; -20°C• 55 μL DNase; -20°C• 600 μL 10X DNase I Buffer; -20°C• 150 μL DNase Inactivation Reagent; -20°C• 5 mL Elution Solution; room temperature
Purification Time:
15 min. (After sample homogenization)
Sample Type:
Cells, Tissue, Laser Capture Microdissection (LCM)
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
50 Preps
Starting Material Amount:
Cells: ≤105Tissue: ≤10 mg
Isolation Technology:
Silica Spin Column
Elution Volume:
10–20 μL
Final Product Type:
Total RNA
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition:
Box 1: Dry IceBox 2: Room Temperature
Content And Storage: Processing Plate & Lid, Binding Solution Concentrate, Wash Solution 1 Concentrate, Wash Solution 2 Concentrate, Elution Buffer are all stored at room temperature. RNA Binding Beads are stored at 4°C and the Lysis ⁄Binding Enhancer is stored at –20°C.
Purification Time: 45 min.
Sample Type: Cell-free Biological Fluids, Liquid Samples (e.g. Serum), Plasma, Serum, Viral Samples
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 50 preps
Starting Material Amount: Up to 400 μl of sample
Isolation Technology: Magnetic Bead
Elution Volume: __
Final Product Type: Viral DNA⁄RNA
For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Shipping Condition: __
Content And Storage:
Processing Plate & Lid, Binding Solution Concentrate, Wash Solution 1 Concentrate, Wash Solution 2 Concentrate, Elution Buffer are all stored at room temperature. RNA Binding Beads are stored at 4°C and the Lysis ⁄Binding Enhancer is stored at –20°C.
Purification Time:
45 min.
Sample Type:
Cell-free Biological Fluids, Liquid Samples (e.g. Serum), Plasma, Serum, Viral Samples
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
50 preps
Starting Material Amount:
Up to 400 μl of sample
Isolation Technology:
Magnetic Bead
Elution Volume:
__
Final Product Type:
Viral DNA⁄RNA
For Use With (Application):
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Shipping Condition:
__
Content And Storage: Store Florescent Substrate, 10X RNaseAlert Lab Test Buffer, and RNase A at -20°C.Store RnaseZap™ and nuclease-free water at room temperature.
Purification Time: __
Sample Type: __
High-throughput Compatibility: __
Quantity: 25 reactions
Starting Material Amount: __
Isolation Technology: __
Elution Volume: __
Final Product Type: __
For Use With (Application): __
Shipping Condition: Dry Ice
Content And Storage:
Store Florescent Substrate, 10X RNaseAlert Lab Test Buffer, and RNase A at -20°C.Store RnaseZap™ and nuclease-free water at room temperature.
Purification Time:
__
Sample Type:
__
High-throughput Compatibility:
__
Quantity:
25 reactions
Starting Material Amount:
__
Isolation Technology:
__
Elution Volume:
__
Final Product Type:
__
For Use With (Application):
__
Shipping Condition:
Dry Ice
Content And Storage: Store RnaseAlert Substrate, 10X Rnase Alert Buffer, Rnase A, and TE Buffer pH 7.0 at -20°C.Store RNaseZap™ Solution and Nuclease-free Water at room temperature.
Purification Time: __
Sample Type: __
High-throughput Compatibility: __
Quantity: 480 Assays
Starting Material Amount: __
Isolation Technology: __
Elution Volume: __
Final Product Type: __
For Use With (Application): __
Shipping Condition: Dry Ice
Content And Storage:
Store RnaseAlert Substrate, 10X Rnase Alert Buffer, Rnase A, and TE Buffer pH 7.0 at -20°C.Store RNaseZap™ Solution and Nuclease-free Water at room temperature.
Purification Time:
__
Sample Type:
__
High-throughput Compatibility:
__
Quantity:
480 Assays
Starting Material Amount:
__
Isolation Technology:
__
Elution Volume:
__
Final Product Type:
__
For Use With (Application):
__
Shipping Condition:
Dry Ice