FERK0802
Thermo Scientific™ GeneJET Plant RNA Purification Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | FERK0802-Each-of-1 | In Stock | ₹ 1,09,559.00 |
FERK0802 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,09,559.00
GST (18%): ₹ 19,720.62
Total Price: ₹ 1,29,279.62
Content And Storage
• 200 mL Plant RNA Lysis Solution; store at room temperature• 200 mL Wash Buffer WB 1 (concentrated); room temperature• 2 x 100 mL Wash Buffer 2 (concentrated); room temperature• 125 mL Water, Nuclease-free; room temperature• 250 GeneJET RNA Spin Columns with Collection Tubes; room temperature• 250 Collection Tubes (2 mL); room temperature• 250 Collection Tubes (1.5 mL); room temperature
Purification Time
20 min. (After sample homogenization)
Sample Type
Plants
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Quantity
250 Preps
Shipping Condition
Room Temperature
Yield
Up to 65 μg
Isolation Technology
Silica Spin Column
Elution Volume
50 μL
Final Product Type
Total RNA
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Scale
Mini
Starting Material Amount
≤100 mg
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Description
- Description GeneJET Plant RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from wide variety of plant species and tissue types
- The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation
- The standard procedure takes less than 20 minutes following cell lysis
- Purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting and other RNA-based analysis
- RNA yields vary between different species, tissues, and age of tissue sample
- See Table 1 for typical total RNA yields from various sources
- Thermo Scientific GeneJET Plant RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from wide variety of plant species and tissue types
- The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation
- The standard procedure takes less than 20 minutes following cell lysis
- Purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting and other RNA-based analysis
- RNA yields vary between different species, tissues, and age of tissue sample
- See Table 1 for typical total RNA yields from various sources
- Highlights • Fast —procedure completed in 20 min following cell lysis • Convinient —protocol does not include clogging-prone lysate filtration step • High yield —up to 65 μg of high purity and high integrity RNA from 50 mg plant sample • Compatible with wide variety of samples (leaves, roots, sprouts and other parts of various plants) Applications Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including: • RT-PCR • RT-qPCR • Northern blotting • Nuclease protection assay
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Content And Storage: • 200 mL Plant RNA Lysis Solution; store at room temperature• 200 mL Wash Buffer WB 1 (concentrated); room temperature• 2 x 100 mL Wash Buffer 2 (concentrated); room temperature• 125 mL Water, Nuclease-free; room temperature• 250 GeneJET RNA Spin Columns with Collection Tubes; room temperature• 250 Collection Tubes (2 mL); room temperature• 250 Collection Tubes (1.5 mL); room temperature
Purification Time: 20 min. (After sample homogenization)
Sample Type: Plants
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 250 Preps
Shipping Condition: Room Temperature
Yield: Up to 65 μg
Isolation Technology: Silica Spin Column
Elution Volume: 50 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Scale: Mini
Starting Material Amount: ≤100 mg
Content And Storage:
• 200 mL Plant RNA Lysis Solution; store at room temperature• 200 mL Wash Buffer WB 1 (concentrated); room temperature• 2 x 100 mL Wash Buffer 2 (concentrated); room temperature• 125 mL Water, Nuclease-free; room temperature• 250 GeneJET RNA Spin Columns with Collection Tubes; room temperature• 250 Collection Tubes (2 mL); room temperature• 250 Collection Tubes (1.5 mL); room temperature
Purification Time:
20 min. (After sample homogenization)
Sample Type:
Plants
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
250 Preps
Shipping Condition:
Room Temperature
Yield:
Up to 65 μg
Isolation Technology:
Silica Spin Column
Elution Volume:
50 μL
Final Product Type:
Total RNA
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Scale:
Mini
Starting Material Amount:
≤100 mg
Content And Storage: • 2 x 1.4 mL Column Preparation Liquid; room temperature• 12 mL Lysis Solution; room temperature• 25 mL Wash Buffer 1 (concentrated); room temperature• 11 mL Wash Buffer 2 (concentrated); room temperature• 3 x 1.25 mL Eluent; room temperature• 50 Spin Columns with Wash Tubes; room temperature• 4 x 50 Wash Tubes; room temperature• 2 x 1.3 mL Proteinase K; –20°C• 1 vial Carrier RNA; –20°C
Purification Time: __
Sample Type: Milk, Plasma, Saliva, Serum, Swabs (Nasal, Buccal, or Urogenital), Urine, Whole Blood, Virus
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 50 Preps
Shipping Condition: Room Temperature
Yield: 50 μg (Binding capacity)
Isolation Technology: Silica Spin Column
Elution Volume: 50 μL
Final Product Type: Viral DNA and RNA
For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
Scale: Mini
Starting Material Amount: 200 μL
Content And Storage:
• 2 x 1.4 mL Column Preparation Liquid; room temperature• 12 mL Lysis Solution; room temperature• 25 mL Wash Buffer 1 (concentrated); room temperature• 11 mL Wash Buffer 2 (concentrated); room temperature• 3 x 1.25 mL Eluent; room temperature• 50 Spin Columns with Wash Tubes; room temperature• 4 x 50 Wash Tubes; room temperature• 2 x 1.3 mL Proteinase K; –20°C• 1 vial Carrier RNA; –20°C
Purification Time:
__
Sample Type:
Milk, Plasma, Saliva, Serum, Swabs (Nasal, Buccal, or Urogenital), Urine, Whole Blood, Virus
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
50 Preps
Shipping Condition:
Room Temperature
Yield:
50 μg (Binding capacity)
Isolation Technology:
Silica Spin Column
Elution Volume:
50 μL
Final Product Type:
Viral DNA and RNA
For Use With (Application):
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
Scale:
Mini
Starting Material Amount:
200 μL
Content And Storage: __
Purification Time: __
Sample Type: DNA, Gel Samples
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 50 Preps
Shipping Condition: __
Yield: __
Isolation Technology: Silica Column
Elution Volume: __
Final Product Type: __
For Use With (Application): Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Nucleic Acid Labeling, Sequencing, PCR, In Vitro Transcription
Scale: __
Starting Material Amount: __
Content And Storage:
__
Purification Time:
__
Sample Type:
DNA, Gel Samples
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
50 Preps
Shipping Condition:
__
Yield:
__
Isolation Technology:
Silica Column
Elution Volume:
__
Final Product Type:
__
For Use With (Application):
Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Nucleic Acid Labeling, Sequencing, PCR, In Vitro Transcription
Scale:
__
Starting Material Amount:
__
Content And Storage: __
Purification Time: __
Sample Type: DNA, Gel Samples
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 250 Preps
Shipping Condition: __
Yield: __
Isolation Technology: Silica Column
Elution Volume: __
Final Product Type: __
For Use With (Application): Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Nucleic Acid Labeling, Sequencing, PCR, In Vitro Transcription
Scale: __
Starting Material Amount: __
Content And Storage:
__
Purification Time:
__
Sample Type:
DNA, Gel Samples
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
250 Preps
Shipping Condition:
__
Yield:
__
Isolation Technology:
Silica Column
Elution Volume:
__
Final Product Type:
__
For Use With (Application):
Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Nucleic Acid Labeling, Sequencing, PCR, In Vitro Transcription
Scale:
__
Starting Material Amount:
__