FERK0732
Thermo Scientific™ GeneJET RNA Purification Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | FERK0732-Each-of-1 | In Stock | ₹ 1,05,495.48 |
FERK0732 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,05,495.48
GST (18%): ₹ 18,989.186
Total Price: ₹ 1,24,484.666
Content And Storage
• 5 x 600 μL Proteinase K; store at room temperature until opened, -20°C after opening• 200 mL Lysis Buffer; room temperature• 200 mL Wash Buffer 1 (concentrated); room temperature• 100 mL Wash Buffer 2 (concentrated); room temperature• 125 mL Nuclease-free Water; room temperature• 250 GeneJET RNA Spin Columns with Collection Tubes; room temperature• 250 Collection Tubes (2 mL); room temperature• 250 Collection Tubes (1.5 mL); room temperature
Purification Time
15 min. (After sample homogenization)
Sample Type
Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Quantity
250 Preps
Shipping Condition
Room Temperature
Yield
Up to 160 μg
Isolation Technology
Silica Spin Column
Elution Volume
50–100 μL
Final Product Type
Total RNA
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Scale
Mini
Starting Material Amount
Bacteria: ≤109cellsBlood: 0.5 mLCells: ≤107Tissue and Insects: 30 mgYeast: ≤4 x 108cellsEnzymatic reactions: ≤100 μL
Related Products
Description
- Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast
- The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions
- RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step
- The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses
- Highlights • Universal —can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria • Efficient —high yields of total RNA
- • Fast —15 minutes after lysis step • Pure —an A260/280 ratio > 1.9 • Convenient —spin columns are capped and assembled with collection tubes Applications Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including: • RT-PCR • RT-qPCR • Northern blotting • Nuclease protection assay Includes Proteinase K Lysis Buffer, Wash Buffer 1 (concentrated), Wash Buffer 2 (concentrated), Nuclease-free Water, GeneJET RNA Purification Columns (pre-assembled with Collection Tubes), Collection Tubes (2 mL), Collection Tubes (1.5 mL), Detailed Protocol
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Content And Storage: • 5 x 600 μL Proteinase K; store at room temperature until opened, -20°C after opening• 200 mL Lysis Buffer; room temperature• 200 mL Wash Buffer 1 (concentrated); room temperature• 100 mL Wash Buffer 2 (concentrated); room temperature• 125 mL Nuclease-free Water; room temperature• 250 GeneJET RNA Spin Columns with Collection Tubes; room temperature• 250 Collection Tubes (2 mL); room temperature• 250 Collection Tubes (1.5 mL); room temperature
Purification Time: 15 min. (After sample homogenization)
Sample Type: Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 250 Preps
Shipping Condition: Room Temperature
Yield: Up to 160 μg
Isolation Technology: Silica Spin Column
Elution Volume: 50–100 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Scale: Mini
Starting Material Amount: Bacteria: ≤109cellsBlood: 0.5 mLCells: ≤107Tissue and Insects: 30 mgYeast: ≤4 x 108cellsEnzymatic reactions: ≤100 μL
Content And Storage:
• 5 x 600 μL Proteinase K; store at room temperature until opened, -20°C after opening• 200 mL Lysis Buffer; room temperature• 200 mL Wash Buffer 1 (concentrated); room temperature• 100 mL Wash Buffer 2 (concentrated); room temperature• 125 mL Nuclease-free Water; room temperature• 250 GeneJET RNA Spin Columns with Collection Tubes; room temperature• 250 Collection Tubes (2 mL); room temperature• 250 Collection Tubes (1.5 mL); room temperature
Purification Time:
15 min. (After sample homogenization)
Sample Type:
Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
250 Preps
Shipping Condition:
Room Temperature
Yield:
Up to 160 μg
Isolation Technology:
Silica Spin Column
Elution Volume:
50–100 μL
Final Product Type:
Total RNA
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Scale:
Mini
Starting Material Amount:
Bacteria: ≤109cellsBlood: 0.5 mLCells: ≤107Tissue and Insects: 30 mgYeast: ≤4 x 108cellsEnzymatic reactions: ≤100 μL
Content And Storage: 5 x 600 μL Proteinase K, Store at room temperature until opened, -20°C after opening, 200 mL Lysis Buffer, 200 mL Wash Buffer 1 (concentrated), 100 mL Wash Buffer 2 (concentrated), 125 mL Nuclease-free Water, 250 GeneJET RNA Spin Columns with Collection Tubes, 250 Collection Tubes (2 mL), 250 Collection Tubes (1.5 mL)
Purification Time: 15 min. (After sample homogenization)
Sample Type: Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 250 Preps
Shipping Condition: Room Temperature
Yield: Up to 160 μg
Isolation Technology: Silica Spin Column
Elution Volume: 50 to 100 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Scale: Mini
Starting Material Amount: __
Content And Storage:
5 x 600 μL Proteinase K, Store at room temperature until opened, -20°C after opening, 200 mL Lysis Buffer, 200 mL Wash Buffer 1 (concentrated), 100 mL Wash Buffer 2 (concentrated), 125 mL Nuclease-free Water, 250 GeneJET RNA Spin Columns with Collection Tubes, 250 Collection Tubes (2 mL), 250 Collection Tubes (1.5 mL)
Purification Time:
15 min. (After sample homogenization)
Sample Type:
Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
250 Preps
Shipping Condition:
Room Temperature
Yield:
Up to 160 μg
Isolation Technology:
Silica Spin Column
Elution Volume:
50 to 100 μL
Final Product Type:
Total RNA
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Scale:
Mini
Starting Material Amount:
__
Content And Storage: __
Purification Time: __
Sample Type: Blood
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 50 Reactions
Shipping Condition: __
Yield: __
Isolation Technology: Spin Column
Elution Volume: __
Final Product Type: __
For Use With (Application): Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Sequencing, PCR
Scale: Mini
Starting Material Amount: __
Content And Storage:
__
Purification Time:
__
Sample Type:
Blood
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
50 Reactions
Shipping Condition:
__
Yield:
__
Isolation Technology:
Spin Column
Elution Volume:
__
Final Product Type:
__
For Use With (Application):
Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Sequencing, PCR
Scale:
Mini
Starting Material Amount:
__
Content And Storage: • 5 x 1.2 mL Proteinase K Solution; store at room temperature until opened, –20°C after opening• 120 mL Lysis Solution; room temperature• 40 mL Wash Buffer I (concentrated); room temperature• 40 mL Wash Buffer II (concentrated); room temperature• 150 mL Elution Buffer; room temperature• 250 GeneJET Purification Columns with Collection Tubes; room temperature• 250 Collection Tubes; room temperature
Purification Time: __
Sample Type: Bone Marrow, Buffy Coat, Dried Blood Spots, Buccal Swab, Urine, Whole Blood
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 250 Preps
Shipping Condition: Room Temperature
Yield: ≤65 μg
Isolation Technology: Silica Spin Column
Elution Volume: 50 to 200 μL
Final Product Type: gDNA
For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
Scale: Mini
Starting Material Amount: Bone Marrow: ≤200 μLBuccal Swabs: 1Buffy Coat: 200 μLDried Blood Spots: variesUrine: ≤4.5 mLWhole Blood: ≤500 μL
Content And Storage:
• 5 x 1.2 mL Proteinase K Solution; store at room temperature until opened, –20°C after opening• 120 mL Lysis Solution; room temperature• 40 mL Wash Buffer I (concentrated); room temperature• 40 mL Wash Buffer II (concentrated); room temperature• 150 mL Elution Buffer; room temperature• 250 GeneJET Purification Columns with Collection Tubes; room temperature• 250 Collection Tubes; room temperature
Purification Time:
__
Sample Type:
Bone Marrow, Buffy Coat, Dried Blood Spots, Buccal Swab, Urine, Whole Blood
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
250 Preps
Shipping Condition:
Room Temperature
Yield:
≤65 μg
Isolation Technology:
Silica Spin Column
Elution Volume:
50 to 200 μL
Final Product Type:
gDNA
For Use With (Application):
PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
Scale:
Mini
Starting Material Amount:
Bone Marrow: ≤200 μLBuccal Swabs: 1Buffy Coat: 200 μLDried Blood Spots: variesUrine: ≤4.5 mLWhole Blood: ≤500 μL