Invitrogen™ PureLink™ RNA Mini Kit

Description

• The PureLink RNA Mini Kit is a column-based kit used to isolate high-quality total RNA from a wide variety of sample types in 20 minutes using standard laboratory equipment
• The kit includes RNase-free lysis and wash solutions that protect RNA from RNases while liberating the RNA from DNA, proteins, and other cellular debris
• The fast spin-column workflow is ideal for processing low to mid-throughput batch sizes
• The advanced PureLink RNA Mini spin column design allows for maximum sample input (200 mg of tissue) and RNA recovery (up to 1000 μg)
• This means that users can process both small and large sample sizes with the same RNA isolation kit
• Process sample inputs from 5–200 mg of animal/plant tissue or from 0.5 x 10 6 to 10 8 tissue culture cells with one spin column in 20 minutes Isolate more RNA than competitor mini spin columns: up to 1000 μg of total RNA from one column Use isolated RNA in most applications without the need for further DNase treatment (very low minimal residual DNA carry over) Flexible–optional on-column or post-isolation DNase treatment is available with PureLink DNase Set (Cat
• No
• 12185010) for easy removal of residual DNA Extra-large binding capacity enables rapid RNA purification using standard laboratory equipment The PureLink RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1000 μg of purified RNA from a single extraction (see Figure 1)
• High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with just trace amounts of residual genomic DNA contamination
• The extra-large binding capacity enables one kit to handle most RNA isolation needs in a quick 20 minute protocol without the need for special sample processing instruments
• Easy, optional, on-column DNase treatment for sensitive applications The PureLink RNA Mini Kit columns are highly efficient for isolating high quality total RNA while removing the majority of genomic DNA
• In general, most applications requiring RNA from animal tissue or mammalian cell lines do not require additional DNase treatment
• However, some applications such as gene expression analysis by qRT-PCR without intron-spanning primers or working with samples from organisms with very small or no introns may require more complete removal of residual contaminating DNA
• The PureLink DNase Set (12185010) allows for convenient on-column digestion of DNA during the RNA isolation protocol
• Treating with DNase while 'on-column' is easier and allows higher RNA recovery than treating with DNase after the RNA has been isolated
• The PureLink DNase Set can also be used to remove residual DNA from RNA that has been previously purified
• Both the 'on-column' and post-RNA purification workflows are options available with the PureLink DNase Set
• Simplified, non-toxic RNA purification The PureLink RNA Mini Kit utilizes non-toxic guanidine-isothiocyanate lysis buffer to protect the RNA during the isolation steps
• Special RNase-free reagents combined with certified RNase-free silica membranes in a unique column configuration allow for a safe and easy procedure that can typically be completed in less than 20 minutes without the need of hazardous phenol/chloroform extraction, CsCl centrifugation, or LiCl or alcohol precipitation
• The PureLink RNA Mini Kit is recommended for use with the Homogenizer (Cat
• No
• 12183026), designed to homogenize cell or tissue lysates via centrifugation, prior to nucleic acid purification
• The Homogenizer is especially effective for clarifying particulates from plant tissues.