A33254

Invitrogen™ TRIzol™ Plus RNA Purification Kit and Phasemaker™ Tubes Complete System

Manufacturer: Invitrogen™

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Pack Size SKU Availability Price
Each of 1 A33254-Each-of-1 In Stock ₹ 42,009.96

A33254 - Each of 1

₹ 42,009.96

In Stock

Quantity

1

Base Price: ₹ 42,009.96

GST (18%): ₹ 7,561.793

Total Price: ₹ 49,571.753

Content And Storage

• 100 mL TRIzol Reagent; room temperature• 100 Phasemaker Tubes; room temperature• 1 PureLink RNA Mini Kit• 125 mL Lysis Buffer; room temperature• 50 mL Wash Buffer I; room temperature• 15 mL Wash Buffer II; room temperature• 15.5 mL RNase-free Water; room temperature• 50 PureLink Spin Cartridges with Collection Tubes; room temperature• 50 Collection Tubes; room temperature• 50 Recovery Tubes; room temperature

Purification Time

1 hr.

Sample Type

Bacteria, Cells, Plants, Tissue, Yeast

High-throughput Compatibility

Not High-throughput Compatible (Manual)

Quantity

50 Preps

Shipping Condition

Room Temperature

Yield

≤350 μg

Isolation Technology

Silica Spin Column, Organic Extraction

Elution Volume

30 to 200 μL

Final Product Type

Total RNA

For Use With (Application)

RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling

Scale

Mini

Starting Material Amount

Bacteria: ≤107Cells: ≤107Plant: ≤107, ≤100 mgTissue: ≤100 mgYeast: ≤107

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Description

  • This is a complete system that includes the TRIzol™ Reagent and the Phasemaker™ Tubes
  • The TRIzol Reagent offers superior lysis capability, even with difficult sample types
  • Invitrogen™ Phasemaker Tubes contain a polymer that clearly separates and completely isolates the upper aqueous phase of the TRIzol mix from the organic phase underneath, making very easy to pipette off the upper phase containing the RNA
  • The polymer works on density gradient, as it is heavier than the aqueous phase, but lighter that the organic phase; after centrifugation it positions itself between these two layers
  • It creates a tight seal between the two phases, making it very easy to pipette off the aqueous phase without the risk of carrying over contaminants from the organic layer
  • Because this, Invitrogen Phasemaker Tubes allow you to recover the whole aqueous phase, increasing yield up to 30%
  • It is an inert polymer that does not interfere with downstream experiments such as PCR
  • It works with fresh or frozen samples (tissue or blood)
  • It also works with tissue samples treated previously with RNAlater
  • Invitrogen Phasemaker Tubes come already pre-dispensed
  • The tube size is 2 mL
  • Complete System includes TRIzol and Phasemaker Tubes Phasemaker Tubes are Pre-dispensed in 2 mL tubes Creates a tight seal between the aqueous and the organic phases Easier to pipette off the whole aqueous phase Increases yield up to 30% RNA integrity is not compromised The seal stays for longer periods of time, allowing to increase the number of samples per batch Inert, does not interfere with downstream experiments Warranty and Services One year after receiving

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Content And Storage:
• 100 mL TRIzol Reagent; room temperature• 100 Phasemaker Tubes; room temperature• 1 PureLink RNA Mini Kit• 125 mL Lysis Buffer; room temperature• 50 mL Wash Buffer I; room temperature• 15 mL Wash Buffer II; room temperature• 15.5 mL RNase-free Water; room temperature• 50 PureLink Spin Cartridges with Collection Tubes; room temperature• 50 Collection Tubes; room temperature• 50 Recovery Tubes; room temperature

Purification Time:
1 hr.

Sample Type:
Bacteria, Cells, Plants, Tissue, Yeast

High-throughput Compatibility:
Not High-throughput Compatible (Manual)

Quantity:
50 Preps

Shipping Condition:
Room Temperature

Yield:
≤350 μg

Isolation Technology:
Silica Spin Column, Organic Extraction

Elution Volume:
30 to 200 μL

Final Product Type:
Total RNA

For Use With (Application):
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling

Scale:
Mini

Starting Material Amount:
Bacteria: ≤107Cells: ≤107Plant: ≤107, ≤100 mgTissue: ≤100 mgYeast: ≤107

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