K157002
Invitrogen™ mRNA Catcher™ PLUS Purification Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K157002-Each-of-1 | In Stock | ₹ 37,646.40 |
K157002 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 37,646.40
GST (18%): ₹ 6,776.352
Total Price: ₹ 44,422.752
Content And Storage
• 1 x 96-well mRNA Catcher™ PLUS Plate; store at 4°C• 4 mL 2X Lysis Buffer L20; room temperature• 30 mL Wash Buffer W15; room temperature• 4 mL Dilution Buffer DL1; room temperature• 8 mL Elution Buffer E3 (DEPC-treated RNase-free water); room temperature• 100 μL 0.5M DTT; 4°C
Purification Time
1.5 hr. (Including sample homogenization)
Sample Type
Cells, Tissue, Total RNA, Whole Blood
High-throughput Compatibility
High-throughput Compatible
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, Microarray Analysis
Shipping Condition
Room Temperature
Yield
>80 ng
Isolation Technology
Oligo dT Affinity
Elution Volume
80 μL
Final Product Type
mRNA
For Use With (Equipment)
Robotic Liquid Handling Systems
Quantity
96 Preps
Starting Material Amount
Cells: 100–106Tissue: 4–40 mgTotal RNA: 0.1–100 μgWhole Blood: 40 μL
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Description
- The mRNA Catcher™ PLUS Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format
- By taking advantage of Locked Nucleic Acid (LNA) technology, the mRNA Catcher PLUS Kit provides improved performance and a faster protocol, while preserving mRNA yield and purity
- How it works mRNA from total RNA, cells, tissue, and blood samples is hybridized to an immobilized, single-stranded 20-mer oligo(dT)-containing LNA
- The incorporation of LNA into the oligo probe increases the hybridization efficiency and specificity of the mRNA binding
- The mRNA can either be eluted or directly reversed-transcribed to cDNA directly in the well
- The mRNA Catcher PLUS method does not require centrifugation, precipitation, phase separation, or column filtration
- In addition, the entire method is amenable to automation for streamlined, high-throughput expression profiling for large-scale processes
- Isolated mRNA is suitable for use in downstream applications such as RT-PCR and qRT-PCR
- • Automated protocols, high-throughput compatible • Robust performance: high binding capacity per well (>80ng/well) • Reproducible results: low CVs from well-to-well and plate-to-plate (∽ 2%) • Optimized protocols: for small sample sizes (100 cells, 4 mg tissue, and 40 μL blood) • Compatible plate design: may be used with most PCR cyclers Order Info Shipping Condition: Wet ice
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Content And Storage: • 1 x 96-well mRNA Catcher™ PLUS Plate; store at 4°C• 4 mL 2X Lysis Buffer L20; room temperature• 30 mL Wash Buffer W15; room temperature• 4 mL Dilution Buffer DL1; room temperature• 8 mL Elution Buffer E3 (DEPC-treated RNase-free water); room temperature• 100 μL 0.5M DTT; 4°C
Purification Time: 1.5 hr. (Including sample homogenization)
Sample Type: Cells, Tissue, Total RNA, Whole Blood
High-throughput Compatibility: High-throughput Compatible
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, Microarray Analysis
Shipping Condition: Room Temperature
Yield: >80 ng
Isolation Technology: Oligo dT Affinity
Elution Volume: 80 μL
Final Product Type: mRNA
For Use With (Equipment): Robotic Liquid Handling Systems
Quantity: 96 Preps
Starting Material Amount: Cells: 100–106Tissue: 4–40 mgTotal RNA: 0.1–100 μgWhole Blood: 40 μL
Content And Storage:
• 1 x 96-well mRNA Catcher™ PLUS Plate; store at 4°C• 4 mL 2X Lysis Buffer L20; room temperature• 30 mL Wash Buffer W15; room temperature• 4 mL Dilution Buffer DL1; room temperature• 8 mL Elution Buffer E3 (DEPC-treated RNase-free water); room temperature• 100 μL 0.5M DTT; 4°C
Purification Time:
1.5 hr. (Including sample homogenization)
Sample Type:
Cells, Tissue, Total RNA, Whole Blood
High-throughput Compatibility:
High-throughput Compatible
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, Microarray Analysis
Shipping Condition:
Room Temperature
Yield:
>80 ng
Isolation Technology:
Oligo dT Affinity
Elution Volume:
80 μL
Final Product Type:
mRNA
For Use With (Equipment):
Robotic Liquid Handling Systems
Quantity:
96 Preps
Starting Material Amount:
Cells: 100–106Tissue: 4–40 mgTotal RNA: 0.1–100 μgWhole Blood: 40 μL
Content And Storage: • 10 96-well mRNA Catcher PLUS Plate; store at 4°C• 40 mL 2X Lysis Buffer L20; room temperature• 2 x 150 mL Wash Buffer W15; room temperature• 40 mL Dilution Buffer DL1; room temperature• 80 mL Elution Buffer E3 (DEPC-treated RNase-free water); room temperature• 1 mL 0.5 M DTT; room temperature
Purification Time: 1.5 hr. (Including sample homogenization)
Sample Type: Cells, Tissue, Total RNA, Whole Blood
High-throughput Compatibility: High-throughput Compatible
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, Microarray Analysis
Shipping Condition: Room Temperature
Yield: >80 ng
Isolation Technology: Oligo dT Affinity
Elution Volume: 80 μL
Final Product Type: mRNA
For Use With (Equipment): Robotic Liquid Handling Systems
Quantity: 960 Preps
Starting Material Amount: Cells: 100–106Tissue: 4–40 mgTotal RNA: 0.1–100 μgWhole Blood: 40 μL
Content And Storage:
• 10 96-well mRNA Catcher PLUS Plate; store at 4°C• 40 mL 2X Lysis Buffer L20; room temperature• 2 x 150 mL Wash Buffer W15; room temperature• 40 mL Dilution Buffer DL1; room temperature• 80 mL Elution Buffer E3 (DEPC-treated RNase-free water); room temperature• 1 mL 0.5 M DTT; room temperature
Purification Time:
1.5 hr. (Including sample homogenization)
Sample Type:
Cells, Tissue, Total RNA, Whole Blood
High-throughput Compatibility:
High-throughput Compatible
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, Microarray Analysis
Shipping Condition:
Room Temperature
Yield:
>80 ng
Isolation Technology:
Oligo dT Affinity
Elution Volume:
80 μL
Final Product Type:
mRNA
For Use With (Equipment):
Robotic Liquid Handling Systems
Quantity:
960 Preps
Starting Material Amount:
Cells: 100–106Tissue: 4–40 mgTotal RNA: 0.1–100 μgWhole Blood: 40 μL
Content And Storage: Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).Includes:• Tango™ OPRK1-bla U2OS DA cells• LiveBLAzer™-FRET B/G Loading Kit, 70 μg• Solution D• Tango™ OPRK1-bla U2OS DA Assay Protocol• LiveBLAzer™-FRET B/G Loading Protocol• Certificate of AnalysisThe Tango™ OPRK1-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Purification Time: __
Sample Type: __
High-throughput Compatibility: __
For Use With (Application): __
Shipping Condition: __
Yield: __
Isolation Technology: __
Elution Volume: __
Final Product Type: __
For Use With (Equipment): __
Quantity: 8 x 106Cells
Starting Material Amount: __
Content And Storage:
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).Includes:• Tango™ OPRK1-bla U2OS DA cells• LiveBLAzer™-FRET B/G Loading Kit, 70 μg• Solution D• Tango™ OPRK1-bla U2OS DA Assay Protocol• LiveBLAzer™-FRET B/G Loading Protocol• Certificate of AnalysisThe Tango™ OPRK1-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Purification Time:
__
Sample Type:
__
High-throughput Compatibility:
__
For Use With (Application):
__
Shipping Condition:
__
Yield:
__
Isolation Technology:
__
Elution Volume:
__
Final Product Type:
__
For Use With (Equipment):
__
Quantity:
8 x 106Cells
Starting Material Amount:
__
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ SSTR5-bla U2OS cells• Tango™ SSTR5-bla U2OS Assay Protocol• Certificate of AnalysisThe Tango™ SSTR5-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Purification Time: __
Sample Type: __
High-throughput Compatibility: __
For Use With (Application): __
Shipping Condition: Room Temperature
Yield: __
Isolation Technology: __
Elution Volume: __
Final Product Type: __
For Use With (Equipment): __
Quantity: 2 x 106Cells
Starting Material Amount: __
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ SSTR5-bla U2OS cells• Tango™ SSTR5-bla U2OS Assay Protocol• Certificate of AnalysisThe Tango™ SSTR5-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Purification Time:
__
Sample Type:
__
High-throughput Compatibility:
__
For Use With (Application):
__
Shipping Condition:
Room Temperature
Yield:
__
Isolation Technology:
__
Elution Volume:
__
Final Product Type:
__
For Use With (Equipment):
__
Quantity:
2 x 106Cells
Starting Material Amount:
__