K157001
Invitrogen™ PureLink™ miRNA Isolation Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K157001-Each-of-1 | In Stock | ₹ 25,839.12 |
K157001 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 25,839.12
GST (18%): ₹ 4,651.042
Total Price: ₹ 30,490.162
Content And Storage
• 45 mL Binding Buffer L3; store at room temperature• 10 mL Wsh Buffer W5; room temperature• 3 mL Buffer W4; room temperature• 5 mL RNase-free Water; room temperature• 50 Spin Cartridges with Collection Tubes; room temperature• 50 Wash Tubes (2 mL); room temperature• 50 Recovery Tubes (1.7 mL); room temperature
Purification Time
20 min. (After sample homogenization)
Sample Type
Bacteria, Cells, Plant, Tissue, Yeast, Dicing Reaction
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Quantity
25 preps
Shipping Condition
Room Temperature
Yield
Up to 6 μg
Isolation Technology
Silica Spin Column
Elution Volume
50–100 μL
Final Product Type
Small RNA
For Use With (Application)
qRT-PCR, Microarray Analysis, Northern Blotting
Scale
Mini
Starting Material Amount
Bacteria: ≤106cellsCells: 106Plant: variesTissue: ≤100 mgYeast: 1 mLDicing Reaction: 300 μL
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Description
- Description The Invitrogen PureLink miRNA Isolation Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total microRNA (miRNA) from a wide variety of sources without the need for hazardous reagents such as phenol
- The kit provides a rapid and convenient method for purifying small RNA molecules from animal and plant cells, as well as from bacteria and yeast samples
- Purify small RNA molecules with a novel enrich/bind/elute procedure Prepare enriched microRNA samples typically in less than 15 minutes Minimize contaminating large RNA molecules and genomic DNA Rapid and Convenient Column Purification Procedure for small RNAs The Invitrogen PureLink miRNA Isolation Kit is specifically designed to purify small RNA molecules, including miRNAs and siRNAs, from a variety of samples typically in less than 15 minutes
- The kit utilizes a silica-based two-column system to enrich exclusively for small RNAs
- RNAs greater than approximately 200 nucleotides are bound to the first column
- Ethanol is then added to the flow-through in order to increase the binding affinity of small RNAs to the glass fiber membrane in the second column
- Once bound to the second column, small RNA molecules are then eluted, ready-for-use, and free of large RNA molecules such as 28S rRNA, 18S rRNA, and mRNA, allowing for focused expression analysis studies of small RNA molecules Purified RNA is Suitable for Use with a Variety of Downstream Applications and Products: The miRNA purified using the Invitrogen PureLink miRNA Isolation Kit is suitable for downstream applications including RNA sequencing, microarray analysis, real-time PCR (qPCR), reverse transcription real-time PCR (RT-qPCR), and northern blotting Not high-throughput compatible (Manual) Applications DNA and RNA Purification and Analysis, RNA Extraction, RNAi, RNAi, Epigenetics and Non-Coding RNA Research, miRNA and Non-Coding RNA Analysis, miRNA Isolation, microRNA Isolation, siRNA Order Info Shipping Condition: Room temperature
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Content And Storage: • 45 mL Binding Buffer L3; store at room temperature• 10 mL Wsh Buffer W5; room temperature• 3 mL Buffer W4; room temperature• 5 mL RNase-free Water; room temperature• 50 Spin Cartridges with Collection Tubes; room temperature• 50 Wash Tubes (2 mL); room temperature• 50 Recovery Tubes (1.7 mL); room temperature
Purification Time: 20 min. (After sample homogenization)
Sample Type: Bacteria, Cells, Plant, Tissue, Yeast, Dicing Reaction
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 25 preps
Shipping Condition: Room Temperature
Yield: Up to 6 μg
Isolation Technology: Silica Spin Column
Elution Volume: 50–100 μL
Final Product Type: Small RNA
For Use With (Application): qRT-PCR, Microarray Analysis, Northern Blotting
Scale: Mini
Starting Material Amount: Bacteria: ≤106cellsCells: 106Plant: variesTissue: ≤100 mgYeast: 1 mLDicing Reaction: 300 μL
Content And Storage:
• 45 mL Binding Buffer L3; store at room temperature• 10 mL Wsh Buffer W5; room temperature• 3 mL Buffer W4; room temperature• 5 mL RNase-free Water; room temperature• 50 Spin Cartridges with Collection Tubes; room temperature• 50 Wash Tubes (2 mL); room temperature• 50 Recovery Tubes (1.7 mL); room temperature
Purification Time:
20 min. (After sample homogenization)
Sample Type:
Bacteria, Cells, Plant, Tissue, Yeast, Dicing Reaction
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
25 preps
Shipping Condition:
Room Temperature
Yield:
Up to 6 μg
Isolation Technology:
Silica Spin Column
Elution Volume:
50–100 μL
Final Product Type:
Small RNA
For Use With (Application):
qRT-PCR, Microarray Analysis, Northern Blotting
Scale:
Mini
Starting Material Amount:
Bacteria: ≤106cellsCells: 106Plant: variesTissue: ≤100 mgYeast: 1 mLDicing Reaction: 300 μL
Content And Storage: • 1 x 96-well mRNA Catcher™ PLUS Plate; store at 4°C• 4 mL 2X Lysis Buffer L20; room temperature• 30 mL Wash Buffer W15; room temperature• 4 mL Dilution Buffer DL1; room temperature• 8 mL Elution Buffer E3 (DEPC-treated RNase-free water); room temperature• 100 μL 0.5M DTT; 4°C
Purification Time: 1.5 hr. (Including sample homogenization)
Sample Type: Cells, Tissue, Total RNA, Whole Blood
High-throughput Compatibility: High-throughput Compatible
Quantity: 96 Preps
Shipping Condition: Room Temperature
Yield: >80 ng
Isolation Technology: Oligo dT Affinity
Elution Volume: 80 μL
Final Product Type: mRNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, Microarray Analysis
Scale: __
Starting Material Amount: Cells: 100–106Tissue: 4–40 mgTotal RNA: 0.1–100 μgWhole Blood: 40 μL
Content And Storage:
• 1 x 96-well mRNA Catcher™ PLUS Plate; store at 4°C• 4 mL 2X Lysis Buffer L20; room temperature• 30 mL Wash Buffer W15; room temperature• 4 mL Dilution Buffer DL1; room temperature• 8 mL Elution Buffer E3 (DEPC-treated RNase-free water); room temperature• 100 μL 0.5M DTT; 4°C
Purification Time:
1.5 hr. (Including sample homogenization)
Sample Type:
Cells, Tissue, Total RNA, Whole Blood
High-throughput Compatibility:
High-throughput Compatible
Quantity:
96 Preps
Shipping Condition:
Room Temperature
Yield:
>80 ng
Isolation Technology:
Oligo dT Affinity
Elution Volume:
80 μL
Final Product Type:
mRNA
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, Microarray Analysis
Scale:
__
Starting Material Amount:
Cells: 100–106Tissue: 4–40 mgTotal RNA: 0.1–100 μgWhole Blood: 40 μL
Content And Storage: • 10 96-well mRNA Catcher PLUS Plate; store at 4°C• 40 mL 2X Lysis Buffer L20; room temperature• 2 x 150 mL Wash Buffer W15; room temperature• 40 mL Dilution Buffer DL1; room temperature• 80 mL Elution Buffer E3 (DEPC-treated RNase-free water); room temperature• 1 mL 0.5 M DTT; room temperature
Purification Time: 1.5 hr. (Including sample homogenization)
Sample Type: Cells, Tissue, Total RNA, Whole Blood
High-throughput Compatibility: High-throughput Compatible
Quantity: 960 Preps
Shipping Condition: Room Temperature
Yield: >80 ng
Isolation Technology: Oligo dT Affinity
Elution Volume: 80 μL
Final Product Type: mRNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, Microarray Analysis
Scale: __
Starting Material Amount: Cells: 100–106Tissue: 4–40 mgTotal RNA: 0.1–100 μgWhole Blood: 40 μL
Content And Storage:
• 10 96-well mRNA Catcher PLUS Plate; store at 4°C• 40 mL 2X Lysis Buffer L20; room temperature• 2 x 150 mL Wash Buffer W15; room temperature• 40 mL Dilution Buffer DL1; room temperature• 80 mL Elution Buffer E3 (DEPC-treated RNase-free water); room temperature• 1 mL 0.5 M DTT; room temperature
Purification Time:
1.5 hr. (Including sample homogenization)
Sample Type:
Cells, Tissue, Total RNA, Whole Blood
High-throughput Compatibility:
High-throughput Compatible
Quantity:
960 Preps
Shipping Condition:
Room Temperature
Yield:
>80 ng
Isolation Technology:
Oligo dT Affinity
Elution Volume:
80 μL
Final Product Type:
mRNA
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, Microarray Analysis
Scale:
__
Starting Material Amount:
Cells: 100–106Tissue: 4–40 mgTotal RNA: 0.1–100 μgWhole Blood: 40 μL
Content And Storage: Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).Includes:• Tango™ OPRK1-bla U2OS DA cells• LiveBLAzer™-FRET B/G Loading Kit, 70 μg• Solution D• Tango™ OPRK1-bla U2OS DA Assay Protocol• LiveBLAzer™-FRET B/G Loading Protocol• Certificate of AnalysisThe Tango™ OPRK1-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Purification Time: __
Sample Type: __
High-throughput Compatibility: __
Quantity: 8 x 106Cells
Shipping Condition: __
Yield: __
Isolation Technology: __
Elution Volume: __
Final Product Type: __
For Use With (Application): __
Scale: __
Starting Material Amount: __
Content And Storage:
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).Includes:• Tango™ OPRK1-bla U2OS DA cells• LiveBLAzer™-FRET B/G Loading Kit, 70 μg• Solution D• Tango™ OPRK1-bla U2OS DA Assay Protocol• LiveBLAzer™-FRET B/G Loading Protocol• Certificate of AnalysisThe Tango™ OPRK1-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Purification Time:
__
Sample Type:
__
High-throughput Compatibility:
__
Quantity:
8 x 106Cells
Shipping Condition:
__
Yield:
__
Isolation Technology:
__
Elution Volume:
__
Final Product Type:
__
For Use With (Application):
__
Scale:
__
Starting Material Amount:
__