Invitrogen™ Glycogen (5 mg/ml)

Description

• Glycogen is a branched chain carbohydrate and is useful as a nucleic acid coprecipitant
• It is provided in five tubes of 1 mL each at a concentration of 5 mg/mL
• Ideal for RT-PCR Increases pellet mass Quantitative recovery of low concentrations (ng/mL) of nucleic acid Prevents pellet loss in nuclease protection assays What is a coprecipitant? Co-precipitants are inert substances used to aid recovery of nucleic acids during alcohol precipitations
• While they can be used for precipitating large amounts of nucleic acids, they are essential for quantitative recovery of small amounts of nucleic acids in dilute solutions
• Often, the use of such molecules is desirable for no other reason but visualization of the pelleted precipitate after centrifugation
• When used at a final concentration of 50 to 150 μg/mL, glycogen will coprecipitate with nucleic acids in the presence of 0.5M ammonium acetate and isopropanol or ethanol
• It is supplied at a concentration of 5mg/mL in diethyl pyrocarbonate-treated distilled water
• Glycogen will not interfere with 260/280 readings
• This glycogen product is isolated from mussel, a biological source, as are most other preparations of this coprecipitant
• Glycogen is treated with Proteinase K and SDS to remove any contaminating nucleases, and then phenol/chloroform extracted, ethanol precipitated, and resuspended in nuclease-free water
• The glycogen is guaranteed RNase- and DNase-free
• Order Info Shipping Condition: Dry Ice