Invitrogen™ Plant RNA Isolation Aid

Description

• Recommended for use with the RNAqueous Kit for purification of total RNA from plant tissues
• One bottle of 10 mL Invitrogen reagent is provided
• Invitrogen's Plant RNA Isolation Aid contains polyvinylpyrrolidone (PVP), a high molecular weight polymer that binds to contaminants such as polyphenolics and polysaccharides that are commonly present in plant tissues
• Glass fiber filter–based methods of isolation, such as the RNAqueous Kit, are especially suited for plant RNA isolation because it does not include any alcohol precipitation steps; many common contaminants found in plant tissue are known to co-precipitate with nucleic acid during alcohol precipitation
• When used with the RNAqueous Kit, the procedure consists of disrupting the plant tissue in a guanidinium-based lysis solution to which the Plant RNA Isolation Aid has been added
• A brief centrifugation then removes the contaminants
• The supernatant is passed through a glass fiber filter which binds RNA
• The filter is then washed and the RNA is eluted in a small volume (50μL or more) of elution solution
• • Removes common plant contaminants such as polyphenolics and polysaccharides • No phenol extraction, alcohol precipitation, or proteinase digestion steps required when used with the RNAqueous Kit • Not high-throughput compatible (Manual) Accessory Product The Plant RNA Isolation Aid is designed for use with the RNAqueous Kit (Cat
• No
• AM1912) for RNA purification from plant tissues
• Applications DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Plant Cells Order Info Shipping Condition: Room temperature