Invitrogen™ Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems

Description

• Description The Invitrogen Collibri Stranded RNA Library Preparation kits are designed for next-generation sequencing (NGS) on Illumina systems
• The kit supports whole-transcriptome sequencing (human, mouse, rat) and mRNA sequencing (compatible with all species) applications
• The Invitrogen Collibri Stranded RNA Library Preparation kits are designed for next-generation sequencing (NGS) on Illumina systems
• The kit supports whole-transcriptome sequencing (human, mouse, rat) and mRNA sequencing (compatible with all species) applications
• The Collibri Stranded RNA Library Preparation Kit: • Includes all necessary components in a single kit* for a quick use • Total RNA converted to sequencing-ready library within 4.5 hours • >98% strand specificity, efficient detection of non-coding RNA and high sensitivity of differential gene expression • Provides in-process visual feedback for easier handling • Is suitable for conventional automation to increase throughput The Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina Systems is designed for robust construction of cDNA libraries for strand-specific RNA sequencing on Illumina next-generation sequencing (NGS) systems
• The kit brings together the superior features of SuperScript IV Reverse Transcriptase, Dynabeads magnetic particles, and Platinum SuperFi DNA Polymerase, enabling users to obtain high-quality sequencing-ready libraries
• This Collibri Stranded RNA Library Prep Kit supports fast library preparation within 4.5 hours for mRNA or whole transcriptome libraries with 1–25 ng of mRNA-enriched or ribodepleted RNA sample input
• Multiplexing of libraries can be carried out using up to 96 single-indexed or unique dual-indexed primers, enabling single-read or paired-end sequencing
• The kit is suitable for various-quality RNA samples, including FFPE samples
• Visual cues of process integrity For maximum convenience, visual cues of process integrity are included throughout the library preparation workflow
• Monitor progress of library generation using visual feedback from dyes in critical reagents
• The reaction mix changes color in response to the addition of critical components at each step to ensure that library preparation has the highest chance of success
• The inert dyes do not interfere with enzymatic reactions and do not compromise sequencing results
• How it works Full-length Illumina-compatible adaptors are introduced by PCR amplification using Platinum SuperFi DNA Polymerase to construct single-indexed or unique dual-indexed libraries compatible with single-read or paired-end sequencing
• The kit contains 24 or 96 either single-indexed barcoded PCR primers (i7) pre-mixed with a universal i5 primer or unique dual-indexed PCR primers that enable multiplexing of up to 96 libraries
• Optimized cleanup steps efficiently remove residual primers and adapter/primer dimers while preserving high library yields
• A related product, the Collibri Library Quantification Kit, is recommended for qPCR-based quantifications of libraries before proceeding to sequencing
• Applications The Collibri Stranded RNA Library Prep Kit is recommended for: • Gene expression studies • Alternative splicing analysis • Non-coding RNA detection and discovery • Identification of alternative polyadenylation sites • Gene fusion detection • Detection of transcript isoforms * The kit consists of enzymatic modification, size selection/clean-up, and indexed adapter-containing modules
• Additional ribodepletion module of human/mouse/rat rRNA can also be included.