AM1905
Invitrogen™ MICROBExpress™ Bacterial mRNA Enrichment Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1905-Each-of-1 | In Stock | ₹ 58,009.68 |
AM1905 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 58,009.68
GST (18%): ₹ 10,441.742
Total Price: ₹ 68,451.422
Content And Storage
Store Control RNA, Capture Oligo Mix, Glycogen, and 3M Sodium Acetate at -20°C.Store Oligo MagBeads, Binding Buffer, and Wash Solution at 4°CStore Nuclease-free Water, 1.5mL tubes, and Collection tubes at room temperature.
Sample Type
Total RNA (Bacterial)
High-throughput Compatibility
Not High-throughput Compatible (Manual)
No. of Reactions
20 Preps
Quantity
20 preps
Isolation Technology
Hybridization Capture, Magnetic Bead
Final Product Type
mRNA (Bacterial)
For Use With (Application)
Microarray Analysis, Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Northern Blotting
Product Line
Ambion™, MICROBEnrich™
Target
Total RNA
Related Products
Description
- The MICROB Express ™ Bacterial mRNA Enrichment Kit is for the purification of bacterial mRNA by removing rRNA from total RNA
- Sufficient reagents for 20 mRNA purifications, each from 10 μg of total RNA are provided
- Features of the MICROB Express ™ Kit: • Dramatically increase the sensitivity of array analyses and other procedures • Removes >95% of 16S and 23S rRNA from bacterial RNA • Simple procedure takes less than 2 hours • Works with many Gram positive and Gram negative bacteria Rapid purification of bacterial mRNA Isolating mRNA from bacteria has been virtually impossible, until now
- The MICROB Express ™ Kit uses a novel technology to remove >95% of the 16S and 23S rRNA from total RNA of E
- coli and other bacterial species
- The kit is suitable for mRNA purification from a broad spectrum of Gram positive and Gram negative bacteria
- The mRNA isolated with the MICROB Express ™ Kit is a superior template for synthesizing labeled cDNA for array analysis, and is ideal for quantitative RT-PCR, northern applications, and cDNA library construction
- Efficient removal of 16S and 23S rRNA from bacterial RNA dramatically increases sensitivity in downstream procedures (see figure)
- Start with any total RNA sample This host-bacterial cell total RNA mixture can be obtained by a variety of RNA isolation methods
- The MICROB Express ™ Kit specifically removes rRNAs; small RNAs (i.e., tRNA and 5S rRNA) are not removed
- Using total RNA that lacks small RNAs as the starting material for MICROB Express ™ Kit produces the highest possible level of mRNA enrichment (see accessory products)
- In the first step of the MICROB Express ™ Kit procedure, total RNA is mixed with an optimized set of capture oligonucleotides that bind to the bacterial 16S and 23S rRNAs
- Next, the rRNA hybrids are removed from the solution using derivatized magnetic microbeads
- The mRNA remains in the supernatant and is recovered by ethanol precipitation
- Accessory products A magnetic stand is required for use this kit
- They are available in several formats, including the Single Place Magnetic Stand (Cat
- No
- AM10026), 6 Tube Magnetic Stand (Cat
- No
- AM10055), and 96-well Magnetic Stands (Cat
- Nos
- AM10027 and AM10050)
- The RiboPure™-Bacteria Kit (Cat
- No
- AM1925) is ideal for the purification of total RNA lacking small RNAs
- Small RNAs can be removed from total RNA using the MEGAclear™ Kit (Cat
- No
- AM1908).
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Content And Storage: Store Control RNA, Capture Oligo Mix, Glycogen, and 3M Sodium Acetate at -20°C.Store Oligo MagBeads, Binding Buffer, and Wash Solution at 4°CStore Nuclease-free Water, 1.5mL tubes, and Collection tubes at room temperature.
Sample Type: Total RNA (Bacterial)
High-throughput Compatibility: Not High-throughput Compatible (Manual)
No. of Reactions: 20 Preps
Quantity: 20 preps
Isolation Technology: Hybridization Capture, Magnetic Bead
Final Product Type: mRNA (Bacterial)
For Use With (Application): Microarray Analysis, Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Northern Blotting
Product Line: Ambion™, MICROBEnrich™
Target: Total RNA
Content And Storage:
Store Control RNA, Capture Oligo Mix, Glycogen, and 3M Sodium Acetate at -20°C.Store Oligo MagBeads, Binding Buffer, and Wash Solution at 4°CStore Nuclease-free Water, 1.5mL tubes, and Collection tubes at room temperature.
Sample Type:
Total RNA (Bacterial)
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
No. of Reactions:
20 Preps
Quantity:
20 preps
Isolation Technology:
Hybridization Capture, Magnetic Bead
Final Product Type:
mRNA (Bacterial)
For Use With (Application):
Microarray Analysis, Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Northern Blotting
Product Line:
Ambion™, MICROBEnrich™
Target:
Total RNA
Content And Storage: Store rDNase1 , 10X DNase 1 Buffer and DNase Inactivation Reagent at -20°C. Store Nuclease–free Water at room temperature.
Sample Type: __
High-throughput Compatibility: __
No. of Reactions: __
Quantity: 50 reactions
Isolation Technology: __
Final Product Type: __
For Use With (Application): __
Product Line: Ambion™, DNA-free™
Target: __
Content And Storage:
Store rDNase1 , 10X DNase 1 Buffer and DNase Inactivation Reagent at -20°C. Store Nuclease–free Water at room temperature.
Sample Type:
__
High-throughput Compatibility:
__
No. of Reactions:
__
Quantity:
50 reactions
Isolation Technology:
__
Final Product Type:
__
For Use With (Application):
__
Product Line:
Ambion™, DNA-free™
Target:
__
Content And Storage: Contains:TURBO DNase10X TURBO DNase BufferDNase Inactivation ReagentNuclease-free WaterStore components at -20°C (Nuclease-free water may be stored at room temperature)
Sample Type: __
High-throughput Compatibility: __
No. of Reactions: __
Quantity: 50 Reactions
Isolation Technology: __
Final Product Type: __
For Use With (Application): __
Product Line: Ambion™, DNA-free™
Target: __
Content And Storage:
Contains:TURBO DNase10X TURBO DNase BufferDNase Inactivation ReagentNuclease-free WaterStore components at -20°C (Nuclease-free water may be stored at room temperature)
Sample Type:
__
High-throughput Compatibility:
__
No. of Reactions:
__
Quantity:
50 Reactions
Isolation Technology:
__
Final Product Type:
__
For Use With (Application):
__
Product Line:
Ambion™, DNA-free™
Target:
__
Content And Storage: Binding Solution, Wash Solution Concentrate, and 5 M Ammonium Acetate are stored at 4°C. Elution Solution can be stored at any temperature, and Filter Cartridges and Collection and Elution Tubes are stored at room temperature.
Sample Type: mRNA, Synthetic mRNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
No. of Reactions: 20 Preps
Quantity: 20 Preps
Isolation Technology: Silica Spin Column
Final Product Type: Purified Synthetic mRNA, Purified mRNA
For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Line: Ambion™, MEGAclear™
Target: __
Content And Storage:
Binding Solution, Wash Solution Concentrate, and 5 M Ammonium Acetate are stored at 4°C. Elution Solution can be stored at any temperature, and Filter Cartridges and Collection and Elution Tubes are stored at room temperature.
Sample Type:
mRNA, Synthetic mRNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
No. of Reactions:
20 Preps
Quantity:
20 Preps
Isolation Technology:
Silica Spin Column
Final Product Type:
Purified Synthetic mRNA, Purified mRNA
For Use With (Application):
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Line:
Ambion™, MEGAclear™
Target:
__