AM1923
Invitrogen™ LeukoLOCK™ Total RNA Isolation System
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1923-Each-of-1 | In Stock | ₹ 92,560.00 |
AM1923 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 92,560.00
GST (18%): ₹ 16,660.80
Total Price: ₹ 1,09,220.80
Content And Storage
Store 1X PBS pH 7.4, RNAlater;™ Solution, Rubber Septum Cap, White Slip Connector, Transfer Spike, LeukoLOCK™ Filter, Elution Solution, LeukoLOCK™ Lysis/Binding Solution, LeukoLOCK™ pH Adjustment Solution, Processing Tubes, Wash Solution 1 Concentrate, Wash Solution 2/3 Concentrate and Nuclease-Free Water at room temperature.Store RNA Binding Beads at 4°C.Store 1X LeukoLOCK™ DNase Buffer, Proteinase K, and TURBO™ DNase at -20°C.
Sample Type
Cells, Blood
High-throughput Compatibility
Not High-throughput Compatible (Manual)
No. of Reactions
20 Preps
Product Line
Ambion™, LeukoLOCK™
Starting Material Amount
Up to 10 ml whole blood
Isolation Technology
Leukocyte Capture Filter, Magnetic Bead
Final Product Type
Total RNA
For Use With (Application)
Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting, cDNA Library Construction
Prep Scale
> 1 mL Blood Samples (Large-scale)
Quantity
20 preps
Target
Total RNA
Related Products
Description
- The LeukoLOCK™ Total RNA Isolation System is an innovative method for cellular fractionation of whole blood and total RNA stabilization and extraction from the leukocyte population
- The kit is comprised of a LeukoLOCK Fractionation & Stabilization Kit (also available separately; Cat
- No
- AM1933) and a LeukoLOCK Total RNA Isolation Kit
- The LeukoLOCK system is optimized for use with human blood
- Blood is a storehouse of cellular information; however, the presence of globin mRNA in RNA prepared from whole blood can interfere with downstream expression profiling applications
- The LeukoLOCK system employs filter-based leukocyte-depletion technology to isolate leukocytes from whole blood and RNA later ™ solution to stabilize the cells on the filter
- By excluding red blood cells, the RNA that is purified from captured leukocytes is inherently depleted of globin mRNA, which improves sample utility for expression profiling and other applications
- Leukocyte Capture and Stabilization A 9–10 mL sample of anticoagulated blood is passed through a LeukoLOCK filter, which captures the total leukocyte population while eliminating red blood cells (including reticulocytes), platelets, and plasma
- After rinsing with phosphate-buffered saline, the filter is flushed with RNA later solution to stabilize the RNA in the captured leukocytes
- The RNA can be isolated immediately, or stabilized cells can be maintained for several days at room temperature or for longer periods at –20°C or –80°C
- RNA Isolation Purification of the RNA is done by first disrupting the captured cells in a guanidinium thiocyanate–based solution that releases the RNA while simultaneously inactivating nucleases
- The cell lysate is collected and briefly treated with Proteinase K
- RNA is then purified from the lysate using MagMAX magnetic bead-based technology for washing, followed by DNase treatment (with TURBO DNase™) and final clean-up
- Expression Studies Both quantitative RT-PCR and microarray expression studies have validated the suitability of the purified RNA for reliable transcriptome profiling
- Compared to RNA processed with a commonly used blood RNA isolation procedure, RNA isolated with the LeukoLOCK system produces longer median aRNA after amplification and higher percent present calls without requiring additional post-extraction steps to remove globin mRNA
- Since globin aRNA represents 25–40% of the aRNA peak from whole blood total RNA, the yield of total RNA is understandably lower with methods that deplete unwanted globin transcripts
- RNA amplified after LeukoLOCK purification offers greater array sensitivity due to the dramatic reduction in competing globin transcripts
- Kit Yields Recovery varies from donor to donor, but is typically 10–20 μg of RNA per 9–10mL of whole blood
- RNA recovered using the LeukoLOCK system contains less than one-tenth the amount of reticulocyte-derived alpha- and beta-globin mRNAs present in typical RNA samples from unfractionated whole blood
- Fractionates leukocytes from whole blood in minutes Extracts total RNA from leukocytes No additional globin reduction steps required Stabilizes RNA to preserve expression profile Preserves leukocytes at room temperature for days Not high-throughput compatible (Manual)
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Content And Storage: Store 1X PBS pH 7.4, RNAlater;™ Solution, Rubber Septum Cap, White Slip Connector, Transfer Spike, LeukoLOCK™ Filter, Elution Solution, LeukoLOCK™ Lysis/Binding Solution, LeukoLOCK™ pH Adjustment Solution, Processing Tubes, Wash Solution 1 Concentrate, Wash Solution 2/3 Concentrate and Nuclease-Free Water at room temperature.Store RNA Binding Beads at 4°C.Store 1X LeukoLOCK™ DNase Buffer, Proteinase K, and TURBO™ DNase at -20°C.
Sample Type: Cells, Blood
High-throughput Compatibility: Not High-throughput Compatible (Manual)
No. of Reactions: 20 Preps
Product Line: Ambion™, LeukoLOCK™
Starting Material Amount: Up to 10 ml whole blood
Isolation Technology: Leukocyte Capture Filter, Magnetic Bead
Final Product Type: Total RNA
For Use With (Application): Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting, cDNA Library Construction
Prep Scale: > 1 mL Blood Samples (Large-scale)
Quantity: 20 preps
Target: Total RNA
Content And Storage:
Store 1X PBS pH 7.4, RNAlater;™ Solution, Rubber Septum Cap, White Slip Connector, Transfer Spike, LeukoLOCK™ Filter, Elution Solution, LeukoLOCK™ Lysis/Binding Solution, LeukoLOCK™ pH Adjustment Solution, Processing Tubes, Wash Solution 1 Concentrate, Wash Solution 2/3 Concentrate and Nuclease-Free Water at room temperature.Store RNA Binding Beads at 4°C.Store 1X LeukoLOCK™ DNase Buffer, Proteinase K, and TURBO™ DNase at -20°C.
Sample Type:
Cells, Blood
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
No. of Reactions:
20 Preps
Product Line:
Ambion™, LeukoLOCK™
Starting Material Amount:
Up to 10 ml whole blood
Isolation Technology:
Leukocyte Capture Filter, Magnetic Bead
Final Product Type:
Total RNA
For Use With (Application):
Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting, cDNA Library Construction
Prep Scale:
> 1 mL Blood Samples (Large-scale)
Quantity:
20 preps
Target:
Total RNA
Content And Storage: __
Sample Type: __
High-throughput Compatibility: __
No. of Reactions: __
Product Line: __
Starting Material Amount: __
Isolation Technology: __
Final Product Type: __
For Use With (Application): __
Prep Scale: __
Quantity: __
Target: __
Content And Storage:
__
Sample Type:
__
High-throughput Compatibility:
__
No. of Reactions:
__
Product Line:
__
Starting Material Amount:
__
Isolation Technology:
__
Final Product Type:
__
For Use With (Application):
__
Prep Scale:
__
Quantity:
__
Target:
__
Content And Storage: • 50 mL TRI Reagent; store at 4°C• 50 Filter Cartridges; room temperature• 100 Collection Tubes (2 mL); room temperature• 60 mL Wash Solution (concentrated); room temperature• 11 mL Elution Buffer; room temperature
Sample Type: Cells, Tissue
High-throughput Compatibility: Not High-throughput Compatible (Manual)
No. of Reactions: __
Product Line: __
Starting Material Amount: Cells: 0.1–20 x 106Tissue: 5–100 mg
Isolation Technology: Spin Column (Glass Fiber Filter)
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Prep Scale: __
Quantity: 50 Preps
Target: __
Content And Storage:
• 50 mL TRI Reagent; store at 4°C• 50 Filter Cartridges; room temperature• 100 Collection Tubes (2 mL); room temperature• 60 mL Wash Solution (concentrated); room temperature• 11 mL Elution Buffer; room temperature
Sample Type:
Cells, Tissue
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
No. of Reactions:
__
Product Line:
__
Starting Material Amount:
Cells: 0.1–20 x 106Tissue: 5–100 mg
Isolation Technology:
Spin Column (Glass Fiber Filter)
Final Product Type:
Total RNA
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Prep Scale:
__
Quantity:
50 Preps
Target:
__
Content And Storage: • 50 1.5-mL Screw Cap Tubes; store at room temperature• 50 Filter Cartridges; room temperature• 100 Collection Tubes (2 mL); room temperature• 24 mL Lysis Buffer; 4°C• 2.4 mL 10% SDS; 4°C• 24 mL Phenol:Chloroform:IAA; 4°C• 95 mL Binding Buffer; 4°C• 35 mL Wash Solution 1; 4°C• 50 mL Wash Solution 2/3 (concentrated); 4°C• 5 mL Elution Solution; 4°C• 80 g Zirconia Beads; -20°C• 550 μL 10X DNase 1 Buffer; -20°C• 200 μL DNase 1; -20°C• 550 μL DNase Inactivation Reagent; -20°C
Sample Type: Yeast
High-throughput Compatibility: Not High-throughput Compatible (Manual)
No. of Reactions: __
Product Line: __
Starting Material Amount: Up to 108cells
Isolation Technology: Spin Column (Glass Fiber Filter)
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Prep Scale: __
Quantity: 50 Preps
Target: __
Content And Storage:
• 50 1.5-mL Screw Cap Tubes; store at room temperature• 50 Filter Cartridges; room temperature• 100 Collection Tubes (2 mL); room temperature• 24 mL Lysis Buffer; 4°C• 2.4 mL 10% SDS; 4°C• 24 mL Phenol:Chloroform:IAA; 4°C• 95 mL Binding Buffer; 4°C• 35 mL Wash Solution 1; 4°C• 50 mL Wash Solution 2/3 (concentrated); 4°C• 5 mL Elution Solution; 4°C• 80 g Zirconia Beads; -20°C• 550 μL 10X DNase 1 Buffer; -20°C• 200 μL DNase 1; -20°C• 550 μL DNase Inactivation Reagent; -20°C
Sample Type:
Yeast
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
No. of Reactions:
__
Product Line:
__
Starting Material Amount:
Up to 108cells
Isolation Technology:
Spin Column (Glass Fiber Filter)
Final Product Type:
Total RNA
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Prep Scale:
__
Quantity:
50 Preps
Target:
__