AM1933
Invitrogen™ LeukoLOCK™ Fractionation & Stabilization Kit with Manual
Manufacturer: Invitrogen™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1933-Each-of-1 | In Stock | ₹ 60,164.00 |
AM1933 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 60,164.00
GST (18%): ₹ 10,829.52
Total Price: ₹ 70,993.52
Concentration
1X
Isolation Technology
Vacuum Filter Column, Magnetic Bead
Final Product Type
Cells (Leukocytes)
For Use With (Application)
Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting
Prep Scale
> 1 mL Blood Samples (Large-scale)
Product Type
Kit
Starting Material Amount
Up to 10 ml whole blood
Yield
20 μg
Content And Storage
The RNAlater™ Solution, Rubber Septum Caps, White Slip Connectors, Transfer Spikes, LeukoLOCK™ Filters, and Elution Solution, should be stored at room temperature. 1X PBS may be stored at any temperature.
Sample Type
Blood
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Includes
RNAlaterSolution, Rubber Septum Caps, White Slip Connectors, Transfer Spikes, LeukoLOCK Filters, and Elution Solution, and 1X PBS
Product Line
Ambion™, LeukoLOCK™
Shipping Condition
Room Temperature
Target
Total RNA
Related Products
Description
- The Ambion™ LeukoLOCK™ Fractionation & Stabilization Kit is one of two kits that comprise the Total RNA Isolation System (patent pending), an innovative method for cellular fractionation of whole blood and total RNA stabilization and extraction from the leukocyte population
- The kit includes sufficient reagents for 20 reactions
- The LeukoLOCK™ System (Cat
- No
- AM1923) is optimized for use with human blood
- Blood is a storehouse of cellular information; however, the presence of globin mRNA in RNA prepared from whole blood can interfere with downstream expression profiling applications
- The LeukoLOCK™ System employs filter-based leukocyte-depletion technology to isolate leukocytes from whole blood and Ambion's RNA later ™ Solution to stabilize the cells on the filter
- By excluding red blood cells, the RNA that is purified from captured leukocytes is inherently depleted of globin mRNA, which improves sample utility for expression profiling and other applications (see Figure)
- LeukoLOCK™ Fractionation & Stabilization Kit A 9–10 mL sample of anticoagulated blood is passed through a LeukoLOCK™ filter, which captures the total leukocyte population while eliminating red blood cells (including reticulocytes), platelets, and plasma
- After rinsing with phosphate-buffered saline, the filter is flushed with RNA later ™ Solutionto stabilize the RNA in the captured leukocytes (see protocol schematic)
- The RNA can be isolated immediately, or stabilized cells can be maintained for several days at room temperature or for longer periods at –20°C or –80°C (see Figure)
- Accessory Products: LeukoLOCK™ Fractionation & Stabilization Kit is designed for use with the LeukoLOCK™ Total RNA Isolation Kit (Cat
- No
- AM1934)
- These kits are offered separately to accommodate blood sample collection at one location and sample processing at a different location, but may be purchased together as the LeukoLOCK™ Total RNA Isolation System (Cat
- No
- AM1923)
- Order Info Shipping Condition: Room temperature
Compare Similar Items
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Concentration: 1X
Isolation Technology: Vacuum Filter Column, Magnetic Bead
Final Product Type: Cells (Leukocytes)
For Use With (Application): Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting
Prep Scale: > 1 mL Blood Samples (Large-scale)
Product Type: Kit
Starting Material Amount: Up to 10 ml whole blood
Yield: 20 μg
Content And Storage: The RNAlater™ Solution, Rubber Septum Caps, White Slip Connectors, Transfer Spikes, LeukoLOCK™ Filters, and Elution Solution, should be stored at room temperature. 1X PBS may be stored at any temperature.
Sample Type: Blood
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Includes: RNAlaterSolution, Rubber Septum Caps, White Slip Connectors, Transfer Spikes, LeukoLOCK Filters, and Elution Solution, and 1X PBS
Product Line: Ambion™, LeukoLOCK™
Shipping Condition: Room Temperature
Target: Total RNA
Concentration:
1X
Isolation Technology:
Vacuum Filter Column, Magnetic Bead
Final Product Type:
Cells (Leukocytes)
For Use With (Application):
Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting
Prep Scale:
> 1 mL Blood Samples (Large-scale)
Product Type:
Kit
Starting Material Amount:
Up to 10 ml whole blood
Yield:
20 μg
Content And Storage:
The RNAlater™ Solution, Rubber Septum Caps, White Slip Connectors, Transfer Spikes, LeukoLOCK™ Filters, and Elution Solution, should be stored at room temperature. 1X PBS may be stored at any temperature.
Sample Type:
Blood
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Includes:
RNAlaterSolution, Rubber Septum Caps, White Slip Connectors, Transfer Spikes, LeukoLOCK Filters, and Elution Solution, and 1X PBS
Product Line:
Ambion™, LeukoLOCK™
Shipping Condition:
Room Temperature
Target:
Total RNA
Concentration: __
Isolation Technology: __
Final Product Type: __
For Use With (Application): __
Prep Scale: __
Product Type: __
Starting Material Amount: __
Yield: __
Content And Storage: __
Sample Type: __
High-throughput Compatibility: __
Includes: __
Product Line: __
Shipping Condition: __
Target: __
Concentration:
__
Isolation Technology:
__
Final Product Type:
__
For Use With (Application):
__
Prep Scale:
__
Product Type:
__
Starting Material Amount:
__
Yield:
__
Content And Storage:
__
Sample Type:
__
High-throughput Compatibility:
__
Includes:
__
Product Line:
__
Shipping Condition:
__
Target:
__
Concentration: __
Isolation Technology: Magnetic Bead
Final Product Type: Viral DNA⁄RNA
For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Prep Scale: __
Product Type: __
Starting Material Amount: Up to 400 μl of sample
Yield: 10 ng
Content And Storage: Processing Plate & Lid, Binding Solution Concentrate, Wash Solution 1 Concentrate, Wash Solution 2 Concentrate, Elution Buffer are all stored at room temperature. RNA Binding Beads are stored at 4°C and the Lysis ⁄Binding Enhancer is stored at –20°C.
Sample Type: Cell-free Biological Fluids, Liquid Samples (e.g. Serum), Plasma, Serum, Viral Samples
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Includes: __
Product Line: MagMAX™
Shipping Condition: __
Target: Viral DNA/RNA
Concentration:
__
Isolation Technology:
Magnetic Bead
Final Product Type:
Viral DNA⁄RNA
For Use With (Application):
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Prep Scale:
__
Product Type:
__
Starting Material Amount:
Up to 400 μl of sample
Yield:
10 ng
Content And Storage:
Processing Plate & Lid, Binding Solution Concentrate, Wash Solution 1 Concentrate, Wash Solution 2 Concentrate, Elution Buffer are all stored at room temperature. RNA Binding Beads are stored at 4°C and the Lysis ⁄Binding Enhancer is stored at –20°C.
Sample Type:
Cell-free Biological Fluids, Liquid Samples (e.g. Serum), Plasma, Serum, Viral Samples
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Includes:
__
Product Line:
MagMAX™
Shipping Condition:
__
Target:
Viral DNA/RNA
Concentration: __
Isolation Technology: Organic Extraction, Spin Column
Final Product Type: Total RNA, micro RNA
For Use With (Application): Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Prep Scale: 100 μL to 1 mL Blood Samples (Medium-scale)
Product Type: Kit
Starting Material Amount: Up to 0.5 ml whole blood
Yield: 40 μg
Content And Storage: Acid-Phenol:Chloroform, 3M Sodium Acetate pH 5.5, Wash Solution 1 Concentrate, Wash Solution 2/3 Concentrate at 4°C. Store 2 mL tubes pre-loaded with RNAlater, Lysis Solution, Filter Cartridges, Collection Tubes, Nuclease-free Water and Elution solution at room temperature.
Sample Type: Blood
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Includes: Acid–Phenol:Chloroform, 3M sodium acetate pH 5.5, wash solution 1 concentrate, wash solution 2/3 concentrate, 2mL tubes pre-loaded with RNAlater, lysis solution, filter cartridges, collection tubes, nuclease-free water and elution solution
Product Line: Ambion™, RiboPure™
Shipping Condition: Room Temperature
Target: miRNA, Total RNA
Concentration:
__
Isolation Technology:
Organic Extraction, Spin Column
Final Product Type:
Total RNA, micro RNA
For Use With (Application):
Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Prep Scale:
100 μL to 1 mL Blood Samples (Medium-scale)
Product Type:
Kit
Starting Material Amount:
Up to 0.5 ml whole blood
Yield:
40 μg
Content And Storage:
Acid-Phenol:Chloroform, 3M Sodium Acetate pH 5.5, Wash Solution 1 Concentrate, Wash Solution 2/3 Concentrate at 4°C. Store 2 mL tubes pre-loaded with RNAlater, Lysis Solution, Filter Cartridges, Collection Tubes, Nuclease-free Water and Elution solution at room temperature.
Sample Type:
Blood
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Includes:
Acid–Phenol:Chloroform, 3M sodium acetate pH 5.5, wash solution 1 concentrate, wash solution 2/3 concentrate, 2mL tubes pre-loaded with RNAlater, lysis solution, filter cartridges, collection tubes, nuclease-free water and elution solution
Product Line:
Ambion™, RiboPure™
Shipping Condition:
Room Temperature
Target:
miRNA, Total RNA