AM1922M
Invitrogen™ Poly(A)Purist™ MAG Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1922M-Each-of-1 | In Stock | ₹ 69,816.96 |
AM1922M - Each of 1
In Stock
Quantity
1
Base Price: ₹ 69,816.96
GST (18%): ₹ 12,567.053
Total Price: ₹ 82,384.013
Content And Storage
Store Nuclease-free Water, Microfuge Tubes and Microfuge tubes with Spin Columns at room temperature. Store 2X Binding Solution, Wash Solution 1, Wash Solution 2 and Oligo(dT) Cellulose at 4°C. Store RNA Storage Solution, 5M Ammonium Acetate and Glycogen(5 mg/mL) at -20°C.
Isolation Technology
Magnetic Bead, Oligo dT Affinity
Final Product Type
mRNA
For Use With (Application)
Microarray Analysis, Reverse Transcriptase PCR (RT-PCR), Microinjection, Nucleic Acid Labeling, In Vitro Translation, cDNA Library Construction, Northern Blotting, Nuclease Protection Assays
Product Type
MAG Kit with Manual
Test Time
45 min.
Format
Kit
Sample Type
RNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Product Line
Ambion™, Poly(A)Purist™
Target
mRNA
Related Products
Description
- Ambion™ Poly(A)Purist™ MAG Kit (patent pending) uses magnetic technology for the selection of mRNA that typically requires only a single round of selection yet yields RNA that is more highly enriched and devoid of rRNA than other procedures
- Each kit contains sufficient reagents for the isolation of mRNA from up to 8 mg of total RNA
- • Highest recovery of mRNA of any available kit • High stringency for lowering residual rRNA content • Single round of selection sufficient for most applications The superior performance of Poly(A)Purist™ MAG Kit is clearly demonstrated by a comparison of the mRNA recovered using 4 other kits (see Figure)
- The kit uses batch binding of RNA to premeasured aliquots of oligo(dT) cellulose to circumvent the problems of slow flow rates and clogged columns often experienced during conventional, gravity-driven chromatography
- The magnetic bead–based selection employed in the delivers increased processing speed, ease of handling, and scalability
- The entire selection procedure can be completed in as little as 45 minutes (see Figure)
- Accessory Products: A magnetic stand is required for use this kit
- They are available in several formats, including the Single Place Magnetic Stand (Cat
- No
- AM10026) and the 6 Tube Magnetic Stand (Cat
- No
- AM10055)
- User Guide Alert (July 2023): Step 3 of the Preparation of Oligo (dT) MagBeads section (page 7) of the User Guide is misleading
- Please follow this instruction for Step 3: add an equivalent mass of Oligo (dT) Magbeads into the selected empty tube and then continue with Oligo (dT) Magbeads preparation according to steps 4-9
- Order Info Shipping Condition: Room Temperature
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Content And Storage: Store Nuclease-free Water, Microfuge Tubes and Microfuge tubes with Spin Columns at room temperature. Store 2X Binding Solution, Wash Solution 1, Wash Solution 2 and Oligo(dT) Cellulose at 4°C. Store RNA Storage Solution, 5M Ammonium Acetate and Glycogen(5 mg/mL) at -20°C.
Isolation Technology: Magnetic Bead, Oligo dT Affinity
Final Product Type: mRNA
For Use With (Application): Microarray Analysis, Reverse Transcriptase PCR (RT-PCR), Microinjection, Nucleic Acid Labeling, In Vitro Translation, cDNA Library Construction, Northern Blotting, Nuclease Protection Assays
Product Type: MAG Kit with Manual
Test Time: 45 min.
Format: Kit
Sample Type: RNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Product Line: Ambion™, Poly(A)Purist™
Target: mRNA
Content And Storage:
Store Nuclease-free Water, Microfuge Tubes and Microfuge tubes with Spin Columns at room temperature. Store 2X Binding Solution, Wash Solution 1, Wash Solution 2 and Oligo(dT) Cellulose at 4°C. Store RNA Storage Solution, 5M Ammonium Acetate and Glycogen(5 mg/mL) at -20°C.
Isolation Technology:
Magnetic Bead, Oligo dT Affinity
Final Product Type:
mRNA
For Use With (Application):
Microarray Analysis, Reverse Transcriptase PCR (RT-PCR), Microinjection, Nucleic Acid Labeling, In Vitro Translation, cDNA Library Construction, Northern Blotting, Nuclease Protection Assays
Product Type:
MAG Kit with Manual
Test Time:
45 min.
Format:
Kit
Sample Type:
RNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Product Line:
Ambion™, Poly(A)Purist™
Target:
mRNA
Content And Storage: Store 1X PBS pH 7.4, RNAlater;™ Solution, Rubber Septum Cap, White Slip Connector, Transfer Spike, LeukoLOCK™ Filter, Elution Solution, LeukoLOCK™ Lysis/Binding Solution, LeukoLOCK™ pH Adjustment Solution, Processing Tubes, Wash Solution 1 Concentrate, Wash Solution 2/3 Concentrate and Nuclease-Free Water at room temperature.Store RNA Binding Beads at 4°C.Store 1X LeukoLOCK™ DNase Buffer, Proteinase K, and TURBO™ DNase at -20°C.
Isolation Technology: Leukocyte Capture Filter, Magnetic Bead
Final Product Type: Total RNA
For Use With (Application): Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting, cDNA Library Construction
Product Type: __
Test Time: __
Format: __
Sample Type: Cells, Blood
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Product Line: Ambion™, LeukoLOCK™
Target: Total RNA
Content And Storage:
Store 1X PBS pH 7.4, RNAlater;™ Solution, Rubber Septum Cap, White Slip Connector, Transfer Spike, LeukoLOCK™ Filter, Elution Solution, LeukoLOCK™ Lysis/Binding Solution, LeukoLOCK™ pH Adjustment Solution, Processing Tubes, Wash Solution 1 Concentrate, Wash Solution 2/3 Concentrate and Nuclease-Free Water at room temperature.Store RNA Binding Beads at 4°C.Store 1X LeukoLOCK™ DNase Buffer, Proteinase K, and TURBO™ DNase at -20°C.
Isolation Technology:
Leukocyte Capture Filter, Magnetic Bead
Final Product Type:
Total RNA
For Use With (Application):
Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting, cDNA Library Construction
Product Type:
__
Test Time:
__
Format:
__
Sample Type:
Cells, Blood
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Product Line:
Ambion™, LeukoLOCK™
Target:
Total RNA
Content And Storage: __
Isolation Technology: __
Final Product Type: __
For Use With (Application): __
Product Type: __
Test Time: __
Format: __
Sample Type: __
High-throughput Compatibility: __
Product Line: __
Target: __
Content And Storage:
__
Isolation Technology:
__
Final Product Type:
__
For Use With (Application):
__
Product Type:
__
Test Time:
__
Format:
__
Sample Type:
__
High-throughput Compatibility:
__
Product Line:
__
Target:
__
Content And Storage: • 50 mL TRI Reagent; store at 4°C• 50 Filter Cartridges; room temperature• 100 Collection Tubes (2 mL); room temperature• 60 mL Wash Solution (concentrated); room temperature• 11 mL Elution Buffer; room temperature
Isolation Technology: Spin Column (Glass Fiber Filter)
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Product Type: __
Test Time: __
Format: __
Sample Type: Cells, Tissue
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Product Line: __
Target: __
Content And Storage:
• 50 mL TRI Reagent; store at 4°C• 50 Filter Cartridges; room temperature• 100 Collection Tubes (2 mL); room temperature• 60 mL Wash Solution (concentrated); room temperature• 11 mL Elution Buffer; room temperature
Isolation Technology:
Spin Column (Glass Fiber Filter)
Final Product Type:
Total RNA
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Product Type:
__
Test Time:
__
Format:
__
Sample Type:
Cells, Tissue
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Product Line:
__
Target:
__