4926385
Invitrogen™ Poly(A)Purist™ MAG Kit
Ambion™ Poly(A)Purist™ MAG Kit (patent pending) uses magnetic technology for the selection of mRNA that typically requires only a single round of selection yet yields RNA that is more highly enriched and devoid of rRNA than other procedures.
Manufacturer: Fischer Scientific
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Content And Storage
Store Nuclease-free Water, Microfuge Tubes and Microfuge tubes with Spin Columns at room temperature. Store 2X Binding Solution, Wash Solution 1, Wash Solution 2 and Oligo(dT) Cellulose at 4°C. Store RNA Storage Solution, 5M Ammonium Acetate and Glycogen(5 mg/mL) at -20°C.
Isolation Technology
Magnetic Bead, Oligo dT Affinity
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Product Line
Ambion™, Poly(A)Purist™
Test Time
45 min.
Format
Kit
Final Product Type
mRNA
For Use With (Application)
Microarray Analysis, Reverse Transcriptase PCR (RT-PCR), Microinjection, Nucleic Acid Labeling, In Vitro Translation, cDNA Library Construction, Northern Blotting, Nuclease Protection Assays
Target
mRNA
Related Products
Description
- Ambion™ Poly(A)Purist™ MAG Kit (patent pending) uses magnetic technology for the selection of mRNA that typically requires only a single round of selection yet yields RNA that is more highly enriched and devoid of rRNA than other procedures
- Each kit contains sufficient reagents for the isolation of mRNA from up to 8 mg of total RNA
- • Highest recovery of mRNA of any available kit • High stringency for lowering residual rRNA content • Single round of selection sufficient for most applications The superior performance of Poly(A)Purist™ MAG Kit is clearly demonstrated by a comparison of the mRNA recovered using 4 other kits (see Figure)
- The kit uses batch binding of RNA to premeasured aliquots of oligo(dT) cellulose to circumvent the problems of slow flow rates and clogged columns often experienced during conventional, gravity-driven chromatography
- The magnetic bead–based selection employed in the delivers increased processing speed, ease of handling, and scalability
- The entire selection procedure can be completed in as little as 45 minutes (see Figure)
- Accessory Products: A magnetic stand is required for use this kit
- They are available in several formats, including the Single Place Magnetic Stand (Cat
- No
- AM10026) and the 6 Tube Magnetic Stand (Cat
- No
- AM10055)
- User Guide Alert (July 2023): Step 3 of the Preparation of Oligo (dT) MagBeads section (page 7) of the User Guide is misleading
- Please follow this instruction for Step 3: add an equivalent mass of Oligo (dT) Magbeads into the selected empty tube and then continue with Oligo (dT) Magbeads preparation according to steps 4-9
- Order Info Shipping Condition: Room Temperature
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Content And Storage: Store Nuclease-free Water, Microfuge Tubes and Microfuge tubes with Spin Columns at room temperature. Store 2X Binding Solution, Wash Solution 1, Wash Solution 2 and Oligo(dT) Cellulose at 4°C. Store RNA Storage Solution, 5M Ammonium Acetate and Glycogen(5 mg/mL) at -20°C.
Isolation Technology: Magnetic Bead, Oligo dT Affinity
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Product Line: Ambion™, Poly(A)Purist™
Test Time: 45 min.
Format: Kit
Final Product Type: mRNA
For Use With (Application): Microarray Analysis, Reverse Transcriptase PCR (RT-PCR), Microinjection, Nucleic Acid Labeling, In Vitro Translation, cDNA Library Construction, Northern Blotting, Nuclease Protection Assays
Target: mRNA
Content And Storage:
Store Nuclease-free Water, Microfuge Tubes and Microfuge tubes with Spin Columns at room temperature. Store 2X Binding Solution, Wash Solution 1, Wash Solution 2 and Oligo(dT) Cellulose at 4°C. Store RNA Storage Solution, 5M Ammonium Acetate and Glycogen(5 mg/mL) at -20°C.
Isolation Technology:
Magnetic Bead, Oligo dT Affinity
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Product Line:
Ambion™, Poly(A)Purist™
Test Time:
45 min.
Format:
Kit
Final Product Type:
mRNA
For Use With (Application):
Microarray Analysis, Reverse Transcriptase PCR (RT-PCR), Microinjection, Nucleic Acid Labeling, In Vitro Translation, cDNA Library Construction, Northern Blotting, Nuclease Protection Assays
Target:
mRNA
Content And Storage: __
Isolation Technology: __
High-throughput Compatibility: High-throughput Compatible, Manual Protocols, Automated Protocols
Product Line: MagMAX™
Test Time: __
Format: Kit
Final Product Type: __
For Use With (Application): Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR)
Target: Genomic DNA
Content And Storage:
__
Isolation Technology:
__
High-throughput Compatibility:
High-throughput Compatible, Manual Protocols, Automated Protocols
Product Line:
MagMAX™
Test Time:
__
Format:
Kit
Final Product Type:
__
For Use With (Application):
Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR)
Target:
Genomic DNA
Content And Storage: __
Isolation Technology: __
High-throughput Compatibility: High-throughput Compatible, Manual Protocols, Automated Protocols
Product Line: MagMAX™
Test Time: __
Format: Kit
Final Product Type: __
For Use With (Application): Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR)
Target: Genomic DNA
Content And Storage:
__
Isolation Technology:
__
High-throughput Compatibility:
High-throughput Compatible, Manual Protocols, Automated Protocols
Product Line:
MagMAX™
Test Time:
__
Format:
Kit
Final Product Type:
__
For Use With (Application):
Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR)
Target:
Genomic DNA
Content And Storage: All components of the ChargeSwitch™ Forensic DNA Purification Kits are shipped at room temperature. Upon receipt, store the Proteinase K at 4°C. Store all other components at room temperature. All components are guaranteed stable for 6 months, if stored properly.Components:• ChargeSwitch™ Lysis Buffer (L13)• ChargeSwitch™ Magnetic Beads• Proteinase K (20 mg/mL in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)• ChargeSwitch™ Purification Buffer (N5)• ChargeSwitch™ Wash Buffer (W12)• ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
Isolation Technology: ChargeSwitch Chemistry
High-throughput Compatibility: High-throughput Compatible
Product Line: ChargeSwitch™
Test Time: __
Format: Kit
Final Product Type: __
For Use With (Application): Forensic
Target: Genomic DNA
Content And Storage:
All components of the ChargeSwitch™ Forensic DNA Purification Kits are shipped at room temperature. Upon receipt, store the Proteinase K at 4°C. Store all other components at room temperature. All components are guaranteed stable for 6 months, if stored properly.Components:• ChargeSwitch™ Lysis Buffer (L13)• ChargeSwitch™ Magnetic Beads• Proteinase K (20 mg/mL in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)• ChargeSwitch™ Purification Buffer (N5)• ChargeSwitch™ Wash Buffer (W12)• ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
Isolation Technology:
ChargeSwitch Chemistry
High-throughput Compatibility:
High-throughput Compatible
Product Line:
ChargeSwitch™
Test Time:
__
Format:
Kit
Final Product Type:
__
For Use With (Application):
Forensic
Target:
Genomic DNA