A1083708
Ambion™ RiboMinus™ Eukaryote Kit for RNA-Seq
Manufacturer: Ambion™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A1083708-Each-of-1 | In Stock | ₹ 76,148.40 |
A1083708 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 76,148.40
GST (18%): ₹ 13,706.712
Total Price: ₹ 89,855.112
Content And Storage
Each kit contains:• 6 mL RiboMinus™ Magnetic Beads• 80 μL RiboMinus™ Eukaryote Probe (15 pmol/μL)• 10 mL Hybridization Buffer• 12 mL DEPC-treated (RNase-free) waterStore at 4°C.
Sample Type
Total RNA
High-throughput Compatibility
High-throughput Compatible
No. of Reactions
8 Preps
Quantity
1 Each
Shipping Condition
Wet Ice
Target
Transcriptome RNA
Yield
1 μg
Isolation Technology
Magnetic Bead
Final Product Type
Transcriptome RNA
For Use With (Application)
Microarray Analysis, Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Sequencing, Northern Blotting, cDNA Library Construction
Product Line
RiboMinus™
Sequencing Type
Transcriptome Sequencing
Starting Material Amount
2-10 μg Total RNA
System Type
RiboMinus
Related Products
Description
- The RiboMinus™ Eukaryote Kit for RNA-Seq provides truly complete transcriptome isolation for next generation sequencing analysis by offering a method for selective depletion of up to 99% of the 5S, 5.8S, 18S and 28S ribosomal RNA from total RNA versus poly-A or cap binding methods, which can introduce bias and select for only part of the transcriptome
- The RiboMinus™ Eukaryote Kit for RNA-Seq Offers: • A simple, yet selective depletion procedure that uses a sequence-specific probe to capture rRNA • Rapid and efficient removal of rRNA-probe complex using magnetic bead technology Three Steps to an rRNA-free Preparation Suitable for Transcriptome Analysis The removal of large ribosomal RNA using the RiboMinus™ kit is a reliable and robust three-step procedure
- Hybridize the RNA sample to the RiboMinus™ Probe in a water bath or heat block, then remove probe-rRNA complexes using the RiboMinus™ magnetic beads included in the kit
- Concentrate the depleted RNA preparation by ethanol precipitation, or by using the RiboMinus™ Concentration Module (Cat
- No
- K155005).
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Content And Storage: Each kit contains:• 6 mL RiboMinus™ Magnetic Beads• 80 μL RiboMinus™ Eukaryote Probe (15 pmol/μL)• 10 mL Hybridization Buffer• 12 mL DEPC-treated (RNase-free) waterStore at 4°C.
Sample Type: Total RNA
High-throughput Compatibility: High-throughput Compatible
No. of Reactions: 8 Preps
Quantity: 1 Each
Shipping Condition: Wet Ice
Target: Transcriptome RNA
Yield: 1 μg
Isolation Technology: Magnetic Bead
Final Product Type: Transcriptome RNA
For Use With (Application): Microarray Analysis, Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Sequencing, Northern Blotting, cDNA Library Construction
Product Line: RiboMinus™
Sequencing Type: Transcriptome Sequencing
Starting Material Amount: 2-10 μg Total RNA
System Type: RiboMinus
Content And Storage:
Each kit contains:• 6 mL RiboMinus™ Magnetic Beads• 80 μL RiboMinus™ Eukaryote Probe (15 pmol/μL)• 10 mL Hybridization Buffer• 12 mL DEPC-treated (RNase-free) waterStore at 4°C.
Sample Type:
Total RNA
High-throughput Compatibility:
High-throughput Compatible
No. of Reactions:
8 Preps
Quantity:
1 Each
Shipping Condition:
Wet Ice
Target:
Transcriptome RNA
Yield:
1 μg
Isolation Technology:
Magnetic Bead
Final Product Type:
Transcriptome RNA
For Use With (Application):
Microarray Analysis, Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Sequencing, Northern Blotting, cDNA Library Construction
Product Line:
RiboMinus™
Sequencing Type:
Transcriptome Sequencing
Starting Material Amount:
2-10 μg Total RNA
System Type:
RiboMinus
Content And Storage: • Ribominus™ Plant Probe, 88 μL• Hybridization Buffer, 11 mL• Rnase Free Water, 13.2 mL• RiboMinus™ Capture Beads, 6 mL• User Manual
Sample Type: Total RNA (Plant)
High-throughput Compatibility: Not High-throughput Compatible (Manual)
No. of Reactions: 8 Preps
Quantity: __
Shipping Condition: Wet Ice
Target: Transcriptome RNA
Yield: 1 μg
Isolation Technology: Magnetic Bead
Final Product Type: Transcriptome RNA
For Use With (Application): Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Sequencing, Northern Blotting, cDNA Library Construction
Product Line: RiboMinus™
Sequencing Type: Transcriptome Sequencing
Starting Material Amount: 2-10 μg Total RNA
System Type: RiboMinus
Content And Storage:
• Ribominus™ Plant Probe, 88 μL• Hybridization Buffer, 11 mL• Rnase Free Water, 13.2 mL• RiboMinus™ Capture Beads, 6 mL• User Manual
Sample Type:
Total RNA (Plant)
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
No. of Reactions:
8 Preps
Quantity:
__
Shipping Condition:
Wet Ice
Target:
Transcriptome RNA
Yield:
1 μg
Isolation Technology:
Magnetic Bead
Final Product Type:
Transcriptome RNA
For Use With (Application):
Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Sequencing, Northern Blotting, cDNA Library Construction
Product Line:
RiboMinus™
Sequencing Type:
Transcriptome Sequencing
Starting Material Amount:
2-10 μg Total RNA
System Type:
RiboMinus
Content And Storage: Contains Alexa Fluor™ 3 FFPE 10X nucleotide mix and Alexa Fluor™ 5 FFPE 10X Nucleotide Mix, Exo-Klenow fragment (40 U⁄μl), 5 mM EDTA, 2.5X Random Primers solution, control DNA (Salmon Sperm), and DEPC-treated water. Store at -80°C for long term storage. The 10X Nucleotide Mixes may be stored at +4°C for up to 4 weeks and should be protected from light. Also contains Purelink™ Genomic DNA purification columns and purification buffers. Store at room temperature. All components are guaranteed for 6 months when properly stored.
Sample Type: Tissue (FFPE)
High-throughput Compatibility: __
No. of Reactions: __
Quantity: 30 Reactions
Shipping Condition: __
Target: __
Yield: __
Isolation Technology: __
Final Product Type: Probes (Labeled DNA)
For Use With (Application): __
Product Line: BioPrime™
Sequencing Type: __
Starting Material Amount: __
System Type: __
Content And Storage:
Contains Alexa Fluor™ 3 FFPE 10X nucleotide mix and Alexa Fluor™ 5 FFPE 10X Nucleotide Mix, Exo-Klenow fragment (40 U⁄μl), 5 mM EDTA, 2.5X Random Primers solution, control DNA (Salmon Sperm), and DEPC-treated water. Store at -80°C for long term storage. The 10X Nucleotide Mixes may be stored at +4°C for up to 4 weeks and should be protected from light. Also contains Purelink™ Genomic DNA purification columns and purification buffers. Store at room temperature. All components are guaranteed for 6 months when properly stored.
Sample Type:
Tissue (FFPE)
High-throughput Compatibility:
__
No. of Reactions:
__
Quantity:
30 Reactions
Shipping Condition:
__
Target:
__
Yield:
__
Isolation Technology:
__
Final Product Type:
Probes (Labeled DNA)
For Use With (Application):
__
Product Line:
BioPrime™
Sequencing Type:
__
Starting Material Amount:
__
System Type:
__
Content And Storage: __
Sample Type: __
High-throughput Compatibility: __
No. of Reactions: __
Quantity: __
Shipping Condition: __
Target: __
Yield: __
Isolation Technology: __
Final Product Type: __
For Use With (Application): Dissections, Forestry applications, Plant botany, Geology, Entomology
Product Line: __
Sequencing Type: __
Starting Material Amount: __
System Type: __
Content And Storage:
__
Sample Type:
__
High-throughput Compatibility:
__
No. of Reactions:
__
Quantity:
__
Shipping Condition:
__
Target:
__
Yield:
__
Isolation Technology:
__
Final Product Type:
__
For Use With (Application):
Dissections, Forestry applications, Plant botany, Geology, Entomology
Product Line:
__
Sequencing Type:
__
Starting Material Amount:
__
System Type:
__