Thermo Scientific™ GeneJET Stabilized and Fresh Whole Blood RNA Kit

Description

• Description The GeneJET™ Stabilized and Fresh Whole Blood RNA Kit is designed for rapid and efficient purification of high quality total RNA from various whole blood samples: fresh, frozen, or human blood samples stabilized with commercial stabilizers such as Tempus™ and PAXgene™
• The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation
• The kit includes a DNase l for the enzymatic removal of genomic DNA
• RNA can be isolated from whole blood in less than 30 minutes (including cell lysis and DNase l treatment)
• Thermo Scientific GeneJET™ Stabilized and Fresh Whole Blood RNA Kit is designed for rapid and efficient purification of high quality total RNA from various whole blood samples: fresh, frozen, or human blood samples stabilized with commercial stabilizers such as Tempus™ and PAXgene™
• The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation
• The kit includes a DNase l for the enzymatic removal of genomic DNA
• RNA can be isolated from whole blood in less than 30 minutes (including cell lysis and DNase l treatment)
• Highlights • Versatile —compatible with fresh and frozen blood, buffy coat, as well as blood collected in PAXgene™and Tempus™ Tubes • Convenient —specially optimized Lysis Buffer is suitable for RNA stabilization up to 48 h • Efficient —up to 4 μg of total RNA from 500 μL of healthy human blood • Pure —isolates intact total RNA free of any RT-qPCR inhibitors Applications Isolated total RNA can be used in: • RT-PCR • RT-qPCR • Northern Blotting Includes • Lysis Buffer (R2841) • Wash Buffer 1 (conc.)(R2851) • Wash Buffer 2 (conc.)(R2861) • DNase l (lyophilized) • DNase I Reconstitution Buffer • 2x DNase I Buffer • Manganese Chloride Solution • Water, nuclease-free • GeneJET RNA Purification Columns pre-assembled with Collection Tubes • Collection Tubes, 2 mL • Collection Tubes, 1.5 mL