BP280550

Fisher BioReagents™ SurePrep™ Nuclear or Cytoplasmic RNA Purification Kit

Manufacturer: Fischer Scientific

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Format

Spin Column

Includes

Lysis solution 20mL, wash solution 22mL, binding solution 25mL, RNA elution solution 6mL, spin columns 50, collection tubes 50, elution tubes 1.7mL 50, product insert 1 50μg Column Binding Capacity, 600μL Max. column lo

Yield

35μg Grape leaves (50mg), 32μg plum leaves (50mg), 60μg tobacco leaves (50mg), 60μg tomato leaves (50mg)

For Use With (Application)

RT-PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension and expression array assays

Quantity

75 Preps

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Description

  • Rapid method for the isolation and purification of both nuclear and cytoplasmic RNA from cultured animal cells and animal tissue samples Fast and easy processing using rapid spin-column format High quality intact RNA can be used in various downstream applications Enrich for cytoplasmic or nuclear RNA depending on downstream application No genomic DNA contamination in the cytoplasmic fraction No phenol or chloroform extractions Isolate all sizes of RNA from each fraction, including all small RNA molecules (>200 nt) Kit provides sufficient reagents to perform either 50 cytoplasmic RNA preparations or 25 cytoplasmic and nuclear preparations Applications: Purification of Nuclear and Cytoplasmic RNA suitable for real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

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Fischer Scientific

BP280550

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Format:
Spin Column

Includes:
Lysis solution 20mL, wash solution 22mL, binding solution 25mL, RNA elution solution 6mL, spin columns 50, collection tubes 50, elution tubes 1.7mL 50, product insert 1 50μg Column Binding Capacity, 600μL Max. column lo

Yield:
35μg Grape leaves (50mg), 32μg plum leaves (50mg), 60μg tobacco leaves (50mg), 60μg tomato leaves (50mg)

For Use With (Application):
RT-PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension and expression array assays

Quantity:
75 Preps

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