Thermo Scientific™ Glycogen, RNA grade

Description

• Glycogen is a highly purified polysaccharide derived from oysters
• It is an inert carrier which significantly increases the recovery of nucleic acids by alcohol precipitation
• Glycogen is insoluble in ethanol and forms a precipitate that traps target nucleic acids
• During centrifugation, a visible pellet is formed which greatly facilitates handling of the precipitated nucleic acids
• Glycogen quantitatively precipitates nucleic acids from diluted solutions with a higher efficiency than that of tRNA, linear polyacrylamide, or sonicated DNA ( see References 1-4)
• We offer two formulations of aqueous glycogen solutions that allow for fast and efficient DNA or RNA recovery: Glycogen, RNA grade , can be used for both RNA and DNA precipitation (#R0551)
• Glycogen, molecular biology grade , recommended for DNA precipitation (#R0561)
• Highlights Ideal for high recovery of oligonucleotides (> 8 bases) and low amounts of DNA/RNA (> 20 pg) from diluted solutions Forms clearly visible pellets Does not interfere with enzymatic reactions† Does not interfere with gel electrophoresis of nucleic acids Does not interfere with spectrophotometrical determination of DNA/RNA concentration, (A 260 to 280 measurements) Compatible with DNA transformation and in vitro transfection