Invitrogen™ EU (5-Ethynyl Uridine)

Description

• Detect global RNA transcription temporally and spatially in both in-vitro and in-vivo with the modified nucleotide, ethynyl uridine
• Approach uses powerful click chemistry detection, a simple and robust two-step technique
• In step one, the alkyne-containing biomolecule is fed to cells or animals and actively incorporated into RNA
• Detection utilizes the chemoselective ligation or 'click' reaction between an azide and an alkyne where the modified protein is detected with a corresponding azide-containing dye or hapten using the Click-iT Cell Reaction Buffer Kit
• Cells can be analyzed by fluorescence microscopy, flow cytometry or high-content imaging and analysis (HCS)
• Cell Analysis, Click Chemistries, Labeling Chemistry, Metabolic Labeling and Nascent Protein Synthesis with Click Chemistry Order Info Shipping Condition: Room temperature