Invitrogen™ EdU (5-ethynyl-2'-deoxyuridine)

Description

• EdU (5-ethynyl-2’-deoxyuridine) can be used as a replacement for BrdU (5-bromo-2’-deoxyuridine) and directly measures de novo DNA synthesis or S-phase synthesis of the cell cycle using click chemistry
• Click chemistry is a method of covalently coupling an azide with an alkyne
• Detection of EdU employs the copper(I) catalyzed click reaction with an azide modified fluorescent dye to form a stable triazole ring
• Because of the small size of the click detection reagent, no harsh denaturation steps are needed to gain access to the DNA
• Eliminating this step allows for more reproducible results, a simpler and quicker protocol and measurements which can easily be multiplexed with relevant antibody based targets including phospho-histone H3, Ki-67, and cyclin B1 as well as dual-pulse experiments with BrdU by flow cytometry, fluorescence microscopy, microplate (HTS) or high-throughput imaging (HCS)
• EdU is readily soluble in DMSO, alcohol, water, or aqueous buffers
• For use with in vitro applications, prepare 10 mM EdU stock solution in DMSO or aqueous buffer
• For Research Use Only
• Not intended for any animal or human therapeutic or diagnostic use
• Order Info Shipping Condition: Room Temperature