11-753-100
Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 11-753-100-Each-of-1 | In Stock | ₹ 81,453.12 |
11-753-100 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 81,453.12
GST (18%): ₹ 14,661.562
Total Price: ₹ 96,114.682
Content And Storage
• 10 mL Resuspension Buffer• 1 mL Lysis Enhancer• 500 μL DNase I, Amplification Grade (1 U/μL)• 160 μL 10X DNase I Buffer• 400 μL 25 mM EDTA• 100 μL SuperScript™ III RT/Platinum™ Taq Mix• 2 × 1.25 mL 2X Reaction Mix• 1 mL 50 mM MgSO4• 2 mL DEPC-treated water• 10 μL HeLa Total RNA (10 ng/μL)• 100 μL ROX™ Reference Dye (store at -20°C in the dark)The kit is shipped on dry ice and should be stored at -20°C
Reaction Speed
Fast or Standard
Product Line
CellsDirect™, Platinum™, SuperScript™
Concentration
2X
Sample Type
Cells
Detection Method
Primer-probe
For Use With (Equipment)
7500 Fast System, 7500 System, BioRad iQ5, BioRad MyiQ, Corbett RotorGene, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad iCycler iQ, Cepheid SmartCycler, MJ Chromo4, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system
For Use With (Application)
RT-PCR
No. of Reactions
100 reactions
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Description
- The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step
- • Efficiency : offers significant time and cost savings for increased throughput • Convenience : enzyme mix includes both SuperScript™ III Reverse Transcriptase and Platinum™ Taq DNA Polymerase • Sensitivity : no separate RNA isolation step increases sensitivity by minimizing sample loss • Broad detection range : detect targets from samples ranging from a single cell to 10,000 cells • Versatility : compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM) Streamline your workflow In traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material
- Using the CellsDirect One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss
- Ideal for your qRT-PCR application CellsDirect technology is validated for gene expression studies involving limited samples
- CellsDirect One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps
- The CellsDirect One-Step qRT-PCR Kit may also be used to detect genomic DNA targets
- CellsDirect One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g., TaqMan™ or LUX™ fluorogenic primers)
- This kit includes a separate tube of ROX™ Reference Dye
- An equivalent kit with ROX Reference Dye included in the 2X Reaction Mix is also available
- Order Info Shipping Condition: Dry ice
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Content And Storage: • 10 mL Resuspension Buffer• 1 mL Lysis Enhancer• 500 μL DNase I, Amplification Grade (1 U/μL)• 160 μL 10X DNase I Buffer• 400 μL 25 mM EDTA• 100 μL SuperScript™ III RT/Platinum™ Taq Mix• 2 × 1.25 mL 2X Reaction Mix• 1 mL 50 mM MgSO4• 2 mL DEPC-treated water• 10 μL HeLa Total RNA (10 ng/μL)• 100 μL ROX™ Reference Dye (store at -20°C in the dark)The kit is shipped on dry ice and should be stored at -20°C
Reaction Speed: Fast or Standard
Product Line: CellsDirect™, Platinum™, SuperScript™
Concentration: 2X
Sample Type: Cells
Detection Method: Primer-probe
For Use With (Equipment): 7500 Fast System, 7500 System, BioRad iQ5, BioRad MyiQ, Corbett RotorGene, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad iCycler iQ, Cepheid SmartCycler, MJ Chromo4, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system
For Use With (Application): RT-PCR
No. of Reactions: 100 reactions
Content And Storage:
• 10 mL Resuspension Buffer• 1 mL Lysis Enhancer• 500 μL DNase I, Amplification Grade (1 U/μL)• 160 μL 10X DNase I Buffer• 400 μL 25 mM EDTA• 100 μL SuperScript™ III RT/Platinum™ Taq Mix• 2 × 1.25 mL 2X Reaction Mix• 1 mL 50 mM MgSO4• 2 mL DEPC-treated water• 10 μL HeLa Total RNA (10 ng/μL)• 100 μL ROX™ Reference Dye (store at -20°C in the dark)The kit is shipped on dry ice and should be stored at -20°C
Reaction Speed:
Fast or Standard
Product Line:
CellsDirect™, Platinum™, SuperScript™
Concentration:
2X
Sample Type:
Cells
Detection Method:
Primer-probe
For Use With (Equipment):
7500 Fast System, 7500 System, BioRad iQ5, BioRad MyiQ, Corbett RotorGene, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad iCycler iQ, Cepheid SmartCycler, MJ Chromo4, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system
For Use With (Application):
RT-PCR
No. of Reactions:
100 reactions
Content And Storage: • 10 mL Resuspension Buffer• 1 mL Lysis Enhancer• 2 × 1.25 mL DNase I, Amplification Grade (1 U/μL)• 800 μL 10X DNase I Buffer• 2 × 1 mL 25 mM EDTA• 500 μL SuperScript™ III RT/Platinum™ Taq Mix• 12.5 mL 2X Reaction Mix• 1 mL 50 mM MgSO4• 12.5 mL DEPC-treated water• 10 μL HeLa Total RNA (10 ng/μL)• 500 μL ROX™ Reference Dye (store at -20°C in the dark)The kit is shipped on dry ice and should be stored at -20°C
Reaction Speed: Fast or Standard
Product Line: CellsDirect™, Platinum™, SuperScript™
Concentration: 2X
Sample Type: Cells
Detection Method: Primer-probe
For Use With (Equipment): 7500 Fast System, 7500 System, BioRad iQ5, BioRad MyiQ, Corbett RotorGene, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad iCycler iQ, Cepheid SmartCycler, MJ Chromo4, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system
For Use With (Application): RT-PCR
No. of Reactions: 500 reactions
Content And Storage:
• 10 mL Resuspension Buffer• 1 mL Lysis Enhancer• 2 × 1.25 mL DNase I, Amplification Grade (1 U/μL)• 800 μL 10X DNase I Buffer• 2 × 1 mL 25 mM EDTA• 500 μL SuperScript™ III RT/Platinum™ Taq Mix• 12.5 mL 2X Reaction Mix• 1 mL 50 mM MgSO4• 12.5 mL DEPC-treated water• 10 μL HeLa Total RNA (10 ng/μL)• 500 μL ROX™ Reference Dye (store at -20°C in the dark)The kit is shipped on dry ice and should be stored at -20°C
Reaction Speed:
Fast or Standard
Product Line:
CellsDirect™, Platinum™, SuperScript™
Concentration:
2X
Sample Type:
Cells
Detection Method:
Primer-probe
For Use With (Equipment):
7500 Fast System, 7500 System, BioRad iQ5, BioRad MyiQ, Corbett RotorGene, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad iCycler iQ, Cepheid SmartCycler, MJ Chromo4, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system
For Use With (Application):
RT-PCR
No. of Reactions:
500 reactions
Content And Storage: __
Reaction Speed: __
Product Line: __
Concentration: __
Sample Type: __
Detection Method: __
For Use With (Equipment): __
For Use With (Application): __
No. of Reactions: __
Content And Storage:
__
Reaction Speed:
__
Product Line:
__
Concentration:
__
Sample Type:
__
Detection Method:
__
For Use With (Equipment):
__
For Use With (Application):
__
No. of Reactions:
__
Content And Storage: • 5X VILO Reaction Mix, 200 μL• 10X SuperScript III Enzyme Blend, 100 μLStore at –5 to –30°C.
Reaction Speed: 60 min.
Product Line: __
Concentration: __
Sample Type: __
Detection Method: qPCR
For Use With (Equipment): __
For Use With (Application): Real Time PCR (qPCR)
No. of Reactions: 50 Reactions
Content And Storage:
• 5X VILO Reaction Mix, 200 μL• 10X SuperScript III Enzyme Blend, 100 μLStore at –5 to –30°C.
Reaction Speed:
60 min.
Product Line:
__
Concentration:
__
Sample Type:
__
Detection Method:
qPCR
For Use With (Equipment):
__
For Use With (Application):
Real Time PCR (qPCR)
No. of Reactions:
50 Reactions