Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit

Description

• The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step
• • Efficiency : offers significant time and cost savings for increased throughput • Convenience : enzyme mix includes both SuperScript™ III Reverse Transcriptase and Platinum™ Taq DNA Polymerase • Sensitivity : no separate RNA isolation step increases sensitivity by minimizing sample loss • Broad detection range : detect targets from samples ranging from a single cell to 10,000 cells • Versatility : compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM) Streamline your workflow In traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material
• Using the CellsDirect One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss
• Ideal for your qRT-PCR application CellsDirect technology is validated for gene expression studies involving limited samples
• CellsDirect One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps
• The CellsDirect One-Step qRT-PCR Kit may also be used to detect genomic DNA targets
• CellsDirect One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g., TaqMan™ or LUX™ fluorogenic primers)
• This kit includes a separate tube of ROX™ Reference Dye
• An equivalent kit with ROX Reference Dye included in the 2X Reaction Mix is also available
• Order Info Shipping Condition: Dry ice