11EPTQKL255

MP Biomedicals™ Taq CORE Kit 25 Direct Loading

Manufacturer: MP Biomedicals™

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Content And Storage

TaqDNA Polymerase 5U/μL (5 x 250U), 5X Direct Loading Buffer with Mg; 5X Direct Loading Buffer without Mg; dNTP mix (25mM each dNTP); 25mM MgCl2. Store at -20°C.

Label or Dye

Red-purple

For Use With (Application)

Standard PCR

Gel Compatibility

Agarose

Quantity

5 x 250U

Concentration

5U/μL

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Description

  • The kits contain MP Biomedicals Taq DNA Polymerase (isolated from Thermus aquaticus ), a thermo-stable recombinant polymerase, with a 5'-3' exonuclease activity, but no 3'- 5' exonuclease activity
  • These kits are also supplied with PCR grade dNTPs, as well as PCR Direct Loading Buffer 5X which contains incubation buffer for Taq DNA Polymerase, a densifying agent and a red-purple dye for direct loading onto agarose gel after PCR cycling
  • The red purple dye migrates at 400 bp on a 1.2% agarose gel.

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MP Biomedicals™

11EPTQKL255

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Content And Storage:
TaqDNA Polymerase 5U/μL (5 x 250U), 5X Direct Loading Buffer with Mg; 5X Direct Loading Buffer without Mg; dNTP mix (25mM each dNTP); 25mM MgCl2. Store at -20°C.

Label or Dye:
Red-purple

For Use With (Application):
Standard PCR

Gel Compatibility:
Agarose

Quantity:
5 x 250U

Concentration:
5U/μL

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11EPTQL100

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Content And Storage:
Store at -20°C

Label or Dye:
__

For Use With (Application):
Standard PCR, RT-PCR, Sequencing, Nucleic Acid Labeling

Gel Compatibility:
__

Quantity:
1000U

Concentration:
5U/μL

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Fischer Scientific

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Content And Storage:
Storage conditions: 2-8° CShipping conditions: AmbientShelf life: 36 months from date of manufacture

Label or Dye:
__

For Use With (Application):
__

Gel Compatibility:
__

Quantity:
10 x 1 L

Concentration:
__

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Fischer Scientific

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Content And Storage:
-20°C

Label or Dye:
__

For Use With (Application):
The optimum amount of antibody (which will influence the number of antibody molecules per particle) depends on the size of the particles (surface area) and on the application. You may need to conjugate different amounts of antibody to optimize your assay. We recommend testing with 0.1 mg/mL in the first instance, although slightly lower or higher concentrations can be explored to optimize performance in your particular application. Before testing antibody concentrations above 0.1 mg/mL we advise you to use our Antibody Concentration & Clean Up Kit for LATEX and EUROPIUM to remove interfering buffer components. This is due to the increase in contamination that will occur when conjugating using a larger volume of the stock antibody. Do not alter the reaction volume of 400 uL as this will reduce conjugation efficiency.

Gel Compatibility:
__

Quantity:
16 μg

Concentration:
__