F130WH

Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)

Manufacturer: Thermo Scientific™

Select a Size

Pack Size SKU Availability Price
Each of 1 F130WH-Each-of-1 In Stock ₹ 38,245.32

F130WH - Each of 1

₹ 38,245.32

In Stock

Quantity

1

Base Price: ₹ 38,245.32

GST (18%): ₹ 6,884.158

Total Price: ₹ 45,129.478

Content And Storage

• Phire Hot Start II DNA Polymerase, 200 μL• 2X Phire Plant Tissue PCR Buffer, 5 x 1.25 mL• Universal Control Primer Mix (25 μM each), 40 μL• Dilution Buffer, 2 x 5 mLStore at -20°C.

PCR Method

Direct PCR

Reaction Speed

Fast

Shipping Condition

Wet or Dry Ice

No. of Reactions

500 Reactions

Reaction Format

Separate Components

Starting Material

DNA

GC-Rich PCR Performance

Medium

Polymerase

Phire Hot Start II DNA Polymerase

Product Type

Direct PCR Kit

Hot Start

Built-In Hot Start

Overhang

Blunt

Sample Type

Plant

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Description

  • Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples
  • No DNA purification is required prior to PCR
  • The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain
  • The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant, fungi, and bacteria
  • The Phire Plant Direct PCR Kit includes a complete set of optimized PCR reagents as well as detailed protocols for different sample types, making it an ideal choice for amplification of plant DNA
  • In addition, the kit includes control primers for positive control reactions
  • The control primers amplify a highly conserved region of chloroplast DNA and are universal for many plant species
  • Features No need for time-consuming and expensive DNA purification steps Very little sample material required Two simple protocols for various applications Phire Hot Start II DNA Polymerase provides high yields of specific product with short extension time (20 s/kb) Applications Genotyping Transgene detection Notes The optimal annealing temperature for Phire DNA Polymerase may differ significantly from that of Taq -based polymerases.

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Thermo Scientific™

F130WH

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Content And Storage:
• Phire Hot Start II DNA Polymerase, 200 μL• 2X Phire Plant Tissue PCR Buffer, 5 x 1.25 mL• Universal Control Primer Mix (25 μM each), 40 μL• Dilution Buffer, 2 x 5 mLStore at -20°C.

PCR Method:
Direct PCR

Reaction Speed:
Fast

Shipping Condition:
Wet or Dry Ice

No. of Reactions:
500 Reactions

Reaction Format:
Separate Components

Starting Material:
DNA

GC-Rich PCR Performance:
Medium

Polymerase:
Phire Hot Start II DNA Polymerase

Product Type:
Direct PCR Kit

Hot Start:
Built-In Hot Start

Overhang:
Blunt

Sample Type:
Plant

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