60-684-050

Invitrogen™ Platinum™TfiExo(-) DNA Polymerase

Manufacturer: Invitrogen™

Select a Size

Pack Size SKU Availability Price
Each of 1 60-684-050-Each-of-1 In Stock ₹ 50,309.28

60-684-050 - Each of 1

₹ 50,309.28

In Stock

Quantity

1

Base Price: ₹ 50,309.28

GST (18%): ₹ 9,055.67

Total Price: ₹ 59,364.95

Content And Storage

Platinum™ Tfi DNA Polymerase (500 units total, at 5U/μL) is supplied with 5X Platinum™ Tfi Reaction Buffer (4 × 1.3 mL) and 50 mM Magnesium Chloride (1 mL). Store at -20°C (non-frost-free).

Polymerase

Tfi DNA Polymerase

Product Line

Platinum™

Quantity

500 units

Concentration

5X

Hot Start

Built-In Hot Start

Reaction Format

Separate Components

Volume

5.2 mL

GC-Rich PCR Performance

Low

Reaction Speed

Standard

Product Type

DNA Polymerase

Shipping Condition

Dry Ice

Fidelity (vs. Taq)

1 X

Overhang

3'-A

Size (Final Product)

1 kb or less

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Description

  • Platinum™ Tfi Exo(-) DNA Polymerase is recombinant Tfi Exo(-) DNA Polymerase complexed with a proprietary antibody mix that inhibits polymerase activity at ambient temperature, allowing room-temperature reaction setup
  • Activity is restored after the initial denaturation step in PCR cycling at 94°C, providing an automatic 'hot start' for increased specificity, sensitivity, and yield
  • Tfi Exo(-) DNA Polymerase is purified from E
  • coli
  • expressing cloned mutants of the Thermus filiformis DNA polymerase gene
  • This enzyme has 5'→3' polymerase activity, but lacks both 5'→3' and 3'→5' exonuclease activity for improved yield and robustness
  • Tfi Exo(-) DNA polymerase is heat-stable and will synthesize DNA at elevated temperatures from single-stranded templates in the presence of a primer
  • Applications Platinum™ Tfi Exo(-) DNA Polymerase can be used in protocols that currently use Platinum™ Taq DNA Polymerase without modification
  • PCR performance is comparable to that of Platinum™ Taq in yield, specificity, fidelity, and robustness
  • Like Platinum™ Taq , Platinum™ Tfi Exo(-) DNA Polymerase has a nontemplate-dependent terminal transferase activity that adds a single deoxyadenosine (A) to the 3' ends of PCR products.

Compare Similar Items

Show Difference

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Invitrogen™

60-684-050

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Content And Storage:
Platinum™ Tfi DNA Polymerase (500 units total, at 5U/μL) is supplied with 5X Platinum™ Tfi Reaction Buffer (4 × 1.3 mL) and 50 mM Magnesium Chloride (1 mL). Store at -20°C (non-frost-free).

Polymerase:
Tfi DNA Polymerase

Product Line:
Platinum™

Quantity:
500 units

Concentration:
5X

Hot Start:
Built-In Hot Start

Reaction Format:
Separate Components

Volume:
5.2 mL

GC-Rich PCR Performance:
Low

Reaction Speed:
Standard

Product Type:
DNA Polymerase

Shipping Condition:
Dry Ice

Fidelity (vs. Taq):
1 X

Overhang:
3'-A

Size (Final Product):
1 kb or less

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GC-Rich PCR Performance:
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Fidelity (vs. Taq):
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GC-Rich PCR Performance:
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GC-Rich PCR Performance:
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Antibody

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__

Overhang:
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__