Invitrogen™ Platinum™ SuperFi II DNA Polymerase

Description

• Invitrogen Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in PCR
• Due to the unique composition of the SuperFi II PCR buffer, the annealing temperature is 60°C for most primer pairs designed following the general design rules
• Features of Platinum SuperFi II DNA Polymerase include: Exceptional >300X Taq fidelity Universal primer annealing at 60°C Superior specificity, sensitivity, and yields Robust amplification of difficult-to-amplify targets Platinum SuperFi II DNA Polymerase is an engineered enzyme with high processivity and increased resistance to PCR inhibitors
• It also enables fast-cycling protocols and amplification of long targets (up to 20 kb)
• Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation
• This technology also enables reaction setup at room temperature and provides increased sensitivity and yield
• Isostabilizing molecules in the buffer increase primer-template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize the annealing temperature for each primer pair
• With Platinum SuperFi II DNA Polymerase, different PCR assays can be cycled together using the same protocol with a universal primer annealing temperature and an extension step selected for the longest fragment to be amplified
• Applications High-fidelity PCR Cloning and sub-cloning Site-directed mutagenesis Amplification of GC-rich templates Template generation for sequencing High-throughput PCR Amplification of samples with suboptimal purity Long PCR