Invitrogen™ Platinum™ SuperFi™ PCR Master Mix

Description

• Invitrogen Platinum SuperFi PCR Master Mix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification
• Only template, primers, and water need to be added, thus reducing the number of pipetting steps during PCR reaction setup
• Platinum SuperFi PCR Master Mix retains all the features of Platinum SuperFi DNA Polymerase, a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology
• Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy
• Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors
• This feature also enables fast-cycling protocols and amplification of long targets
• The Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation
• Features Exceptional >300X Taq fidelity High specificity and increased yields with Platinum hot-start technology Robust amplification of difficult targets of high GC content Room temperature reaction setup and 24-hour bench top stability of pre-assembled reactions Amplification of samples with suboptimal purity Applications High-fidelity PCR Cloning and sub-cloning Site-directed mutagenesis Amplification of GC-rich templates Template generation for sequencing High-throughput PCR Long PCR Notes Annealing rules for Platinum SuperFi DNA Polymerase are different from many common DNA polymerases
• Platinum SuperFi PCR Master Mix is supplied with separate SuperFi GC Enhancer for difficult and GC-rich templates (˃65% GC).