Thermo Scientific™ Phusion™ Plus PCR Master Mixes

Description

• Thermo Scientific Phusion Plus Green PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs
• The master mix format simplifies reaction setup with minimal pipetting steps and retains all features of Phusion Plus DNA Polymerase
• Additionally, two tracking dyes and a density reagent are pre-added to the master mix for direct loading of PCR products on gels
• Features of Phusion™ Plus PCR Master Mix include: High fidelity —provides >100X higher sequence accuracy than Taq polymerase Convenient setup —enables a universal annealing temperature of 60°C in PCR Enhanced specificity —hot-start mechanism reduces non-specific amplification and primer degradation Benchtop stability —automation enabled due to 24-hour stability of assembled reactions High inhibitor tolerance —works with DNA of suboptimal purity Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing
• It is a Pyrococcus-like proofreading DNA polymerase fused to a processivity-enhancing domain, enabling Phusion Plus PCR Master Mix to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance
• Its novel reaction buffer includes proprietary additives that stabilize primer/template duplexes during the annealing step at 60°C
• This unique buffer formulation increases PCR specificity without the need to optimize annealing temperature for each primer pair, helping save time and avoid mistakes in PCR runs
• Furthermore, with Phusion Plus PCR Master Mix and its universal annealing feature, different PCR reactions can be cycled together using the same protocol
• Phusion Plus PCR Master Mix is inactive at ambient temperatures due to its hot-start mechanism mediated by Affibody molecules, allowing setup and storage of assembled PCR reactions at room temperature
• Enzyme activity is fully restored after the initial denaturation step
• Applications High-fidelity PCR Cloning Template generation for sequencing Amplification of difficult (GC-rich) templates Long-range PCR (up to 20 kb) Mutagenesis High-throughput PCR