F631S
Thermo Scientific™ Phusion™ Plus PCR Master Mixes
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | F631S-Each-of-1 | In Stock | ₹ 27,100.50 |
F631S - Each of 1
In Stock
Quantity
1
Base Price: ₹ 27,100.50
GST (18%): ₹ 4,878.09
Total Price: ₹ 31,978.59
Content And Storage
• 2X Phusion Plus PCR Master Mix (2 x 1.25 mL)• 5X Phusion GC Enhancer (1.25 mL)• Nuclease-free water (2 x 1.25 mL)Store at –15°C to –25°C
Polymerase
Phusion Plus DNA Polymerase
Shipping Condition
Dry Ice
Concentration
2X
Hot Start
Built-In Hot Start
Overhang
Blunt
Size (Final Product)
20 kb or less
GC-Rich PCR Performance
High
Reaction Speed
15 to 30 sec./kb
For Use With (Application)
Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq)
>100X
No. of Reactions
100 Reactions
Reaction Format
Ready to use master mix
Color
Colorless
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Description
- Thermo Scientific Phusion Plus Green PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs
- The master mix format simplifies reaction setup with minimal pipetting steps and retains all features of Phusion Plus DNA Polymerase
- Additionally, two tracking dyes and a density reagent are pre-added to the master mix for direct loading of PCR products on gels
- Features of Phusion™ Plus PCR Master Mix include: High fidelity —provides >100X higher sequence accuracy than Taq polymerase Convenient setup —enables a universal annealing temperature of 60°C in PCR Enhanced specificity —hot-start mechanism reduces non-specific amplification and primer degradation Benchtop stability —automation enabled due to 24-hour stability of assembled reactions High inhibitor tolerance —works with DNA of suboptimal purity Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing
- It is a Pyrococcus-like proofreading DNA polymerase fused to a processivity-enhancing domain, enabling Phusion Plus PCR Master Mix to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance
- Its novel reaction buffer includes proprietary additives that stabilize primer/template duplexes during the annealing step at 60°C
- This unique buffer formulation increases PCR specificity without the need to optimize annealing temperature for each primer pair, helping save time and avoid mistakes in PCR runs
- Furthermore, with Phusion Plus PCR Master Mix and its universal annealing feature, different PCR reactions can be cycled together using the same protocol
- Phusion Plus PCR Master Mix is inactive at ambient temperatures due to its hot-start mechanism mediated by Affibody molecules, allowing setup and storage of assembled PCR reactions at room temperature
- Enzyme activity is fully restored after the initial denaturation step
- Applications High-fidelity PCR Cloning Template generation for sequencing Amplification of difficult (GC-rich) templates Long-range PCR (up to 20 kb) Mutagenesis High-throughput PCR
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Content And Storage: • 2X Phusion Plus PCR Master Mix (2 x 1.25 mL)• 5X Phusion GC Enhancer (1.25 mL)• Nuclease-free water (2 x 1.25 mL)Store at –15°C to –25°C
Polymerase: Phusion Plus DNA Polymerase
Shipping Condition: Dry Ice
Concentration: 2X
Hot Start: Built-In Hot Start
Overhang: Blunt
Size (Final Product): 20 kb or less
GC-Rich PCR Performance: High
Reaction Speed: 15 to 30 sec./kb
For Use With (Application): Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq): >100X
No. of Reactions: 100 Reactions
Reaction Format: Ready to use master mix
Color: Colorless
Content And Storage:
• 2X Phusion Plus PCR Master Mix (2 x 1.25 mL)• 5X Phusion GC Enhancer (1.25 mL)• Nuclease-free water (2 x 1.25 mL)Store at –15°C to –25°C
Polymerase:
Phusion Plus DNA Polymerase
Shipping Condition:
Dry Ice
Concentration:
2X
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Size (Final Product):
20 kb or less
GC-Rich PCR Performance:
High
Reaction Speed:
15 to 30 sec./kb
For Use With (Application):
Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq):
>100X
No. of Reactions:
100 Reactions
Reaction Format:
Ready to use master mix
Color:
Colorless
Content And Storage: 2X Phusion Plus PCR Master Mix (2 x 1.25 mL), 5X Phusion GC Enhancer (1.25 mL), Nuclease-free water (2 x 1.25 mL), Store at -15°C to -25°C
Polymerase: Phusion Plus DNA Polymerase
Shipping Condition: Dry Ice
Concentration: 2X
Hot Start: Built-In Hot Start
Overhang: Blunt
Size (Final Product): 20 kb or less
GC-Rich PCR Performance: High
Reaction Speed: 15 to 30 sec./kb
For Use With (Application): Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq): >100X
No. of Reactions: 100 Reactions
Reaction Format: Ready to use master mix
Color: __
Content And Storage:
2X Phusion Plus PCR Master Mix (2 x 1.25 mL), 5X Phusion GC Enhancer (1.25 mL), Nuclease-free water (2 x 1.25 mL), Store at -15°C to -25°C
Polymerase:
Phusion Plus DNA Polymerase
Shipping Condition:
Dry Ice
Concentration:
2X
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Size (Final Product):
20 kb or less
GC-Rich PCR Performance:
High
Reaction Speed:
15 to 30 sec./kb
For Use With (Application):
Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq):
>100X
No. of Reactions:
100 Reactions
Reaction Format:
Ready to use master mix
Color:
__
Content And Storage: Four packs, each containing:• 2X Phusion Plus PCR Master Mix (10 x 1.25 mL)• 5X Phusion GC Enhancer (4 x 1.25 mL)• Nuclease-free water (10 x 1.25 mL)Store at –15°C to –25°C
Polymerase: Phusion Plus DNA Polymerase
Shipping Condition: Dry Ice
Concentration: 2X
Hot Start: Built-In Hot Start
Overhang: Blunt
Size (Final Product): 20 kb or less
GC-Rich PCR Performance: High
Reaction Speed: 15 to 30 sec./kb
For Use With (Application): Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq): >100X
No. of Reactions: 2000 Reactions
Reaction Format: Ready to use master mix
Color: Colorless
Content And Storage:
Four packs, each containing:• 2X Phusion Plus PCR Master Mix (10 x 1.25 mL)• 5X Phusion GC Enhancer (4 x 1.25 mL)• Nuclease-free water (10 x 1.25 mL)Store at –15°C to –25°C
Polymerase:
Phusion Plus DNA Polymerase
Shipping Condition:
Dry Ice
Concentration:
2X
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Size (Final Product):
20 kb or less
GC-Rich PCR Performance:
High
Reaction Speed:
15 to 30 sec./kb
For Use With (Application):
Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq):
>100X
No. of Reactions:
2000 Reactions
Reaction Format:
Ready to use master mix
Color:
Colorless
Content And Storage: • 2X Phusion Plus Green PCR Master Mix (10 x 1.25 mL)• 5X Phusion GC Enhancer (4 x 1.25 mL)• Nuclease-free water (10 x 1.25 mL)Store at –15°C to –25°C.
Polymerase: Phusion Plus DNA Polymerase
Shipping Condition: Dry Ice
Concentration: 2X
Hot Start: Built-In Hot Start
Overhang: Blunt
Size (Final Product): 20 kb or less
GC-Rich PCR Performance: High
Reaction Speed: 15 to 30 sec./kb
For Use With (Application): Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq): >100X
No. of Reactions: 500 Reactions
Reaction Format: Ready to use master mix
Color: Green
Content And Storage:
• 2X Phusion Plus Green PCR Master Mix (10 x 1.25 mL)• 5X Phusion GC Enhancer (4 x 1.25 mL)• Nuclease-free water (10 x 1.25 mL)Store at –15°C to –25°C.
Polymerase:
Phusion Plus DNA Polymerase
Shipping Condition:
Dry Ice
Concentration:
2X
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Size (Final Product):
20 kb or less
GC-Rich PCR Performance:
High
Reaction Speed:
15 to 30 sec./kb
For Use With (Application):
Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq):
>100X
No. of Reactions:
500 Reactions
Reaction Format:
Ready to use master mix
Color:
Green