F630S
Thermo Scientific™ Phusion™ Plus DNA Polymerase
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | F630S-Each-of-1 | In Stock | ₹ 14,784.77 |
F630S - Each of 1
In Stock
Quantity
1
Base Price: ₹ 14,784.77
GST (18%): ₹ 2,661.259
Total Price: ₹ 17,446.029
Content And Storage
• Phusion Plus DNA Polymerase (50 μL)• 5X Phusion Plus Buffer (1.25 mL)• 5X Phusion GC Enhancer (1.25 mL)Store at –15°C to –25°C.
Polymerase
Phusion Plus DNA Polymerase
Shipping Condition
Dry Ice
Fidelity (vs. Taq)
>100X
No. of Reactions
100 Reactions
Reaction Format
Separate components
GC-Rich PCR Performance
High
Reaction Speed
15 to 30 sec./kb
For Use With (Application)
Hot-start PCR, High-fidelity PCR
Hot Start
Built-In Hot Start
Overhang
Blunt
Size (Final Product)
20 kb or less
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Description
- Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing
- It is a Pyrococcus -like proofreading DNA polymerase fused to a processivity-enhancing domain, enabling Phusion Plus DNA Polymerase to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance
- Features include: >100X higher sequence accuracy than Taq polymerase Universal annealing temperature of 60°C in PCR Hot-start mechanism reduces non-specific amplification and primer degradation Automation enabled due to 24-hour stability of assembled reactions High inhibitor tolerance for work with DNA of suboptimal purity The novel Phusion Plus reaction buffer includes proprietary additives that stabilize primer/template duplexes during the annealing step at 60°C
- This unique buffer formulation increases PCR specificity without the need to optimize annealing temperature for each primer pair, helping save time and avoid mistakes in PCR runs
- Furthermore, with Phusion Plus DNA Polymerase and its universal annealing feature, different PCR reactions can be cycled together using the same protocol
- Phusion Plus DNA Polymerase is inactive at ambient temperatures due to its hot-start mechanism mediated by Affibody molecules, allowing setup and storage of assembled PCR reactions at room temperature
- Enzyme activity is fully restored after the initial denaturation step
- Applications High-fidelity PCR Cloning & mutagenesis Template generation for sequencing GC-rich PCR Long-range PCR (up to 20 kb) High-throughput PCR Notes To simplify reactrion setup, Phusion Plus DNA Polymerase is also available in ready-to-use master mix formats.
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Content And Storage: • Phusion Plus DNA Polymerase (50 μL)• 5X Phusion Plus Buffer (1.25 mL)• 5X Phusion GC Enhancer (1.25 mL)Store at –15°C to –25°C.
Polymerase: Phusion Plus DNA Polymerase
Shipping Condition: Dry Ice
Fidelity (vs. Taq): >100X
No. of Reactions: 100 Reactions
Reaction Format: Separate components
GC-Rich PCR Performance: High
Reaction Speed: 15 to 30 sec./kb
For Use With (Application): Hot-start PCR, High-fidelity PCR
Hot Start: Built-In Hot Start
Overhang: Blunt
Size (Final Product): 20 kb or less
Content And Storage:
• Phusion Plus DNA Polymerase (50 μL)• 5X Phusion Plus Buffer (1.25 mL)• 5X Phusion GC Enhancer (1.25 mL)Store at –15°C to –25°C.
Polymerase:
Phusion Plus DNA Polymerase
Shipping Condition:
Dry Ice
Fidelity (vs. Taq):
>100X
No. of Reactions:
100 Reactions
Reaction Format:
Separate components
GC-Rich PCR Performance:
High
Reaction Speed:
15 to 30 sec./kb
For Use With (Application):
Hot-start PCR, High-fidelity PCR
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Size (Final Product):
20 kb or less
Content And Storage: Phusion Plus DNA Polymerase (50 μL), 5X Phusion Plus Buffer (1.25 mL), 5X Phusion GC Enhancer (1.25 mL), Store at -15°C to -25°C.
Polymerase: Phusion Plus DNA Polymerase
Shipping Condition: Dry Ice
Fidelity (vs. Taq): >100X
No. of Reactions: 100 Reactions
Reaction Format: Separate components
GC-Rich PCR Performance: High
Reaction Speed: 15 to 30 sec./kb
For Use With (Application): Hot-start PCR, High-fidelity PCR
Hot Start: Built-In Hot Start
Overhang: Blunt
Size (Final Product): 20 kb or less
Content And Storage:
Phusion Plus DNA Polymerase (50 μL), 5X Phusion Plus Buffer (1.25 mL), 5X Phusion GC Enhancer (1.25 mL), Store at -15°C to -25°C.
Polymerase:
Phusion Plus DNA Polymerase
Shipping Condition:
Dry Ice
Fidelity (vs. Taq):
>100X
No. of Reactions:
100 Reactions
Reaction Format:
Separate components
GC-Rich PCR Performance:
High
Reaction Speed:
15 to 30 sec./kb
For Use With (Application):
Hot-start PCR, High-fidelity PCR
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Size (Final Product):
20 kb or less
Content And Storage: Four packs, each containing:• Phusion Plus DNA Polymerase (250 μL)• 5X Phusion Plus Buffer (5 x 1.25 mL)• 5X Phusion GC Enhancer (4 x 1.25 mL)Store at –15°C to –25°C.
Polymerase: Phusion Plus DNA Polymerase
Shipping Condition: Dry Ice
Fidelity (vs. Taq): >100X
No. of Reactions: 2000 Reactions
Reaction Format: Separate components
GC-Rich PCR Performance: High
Reaction Speed: 15 to 30 sec./kb
For Use With (Application): Hot-start PCR, High-fidelity PCR
Hot Start: Built-In Hot Start
Overhang: Blunt
Size (Final Product): 20 kb or less
Content And Storage:
Four packs, each containing:• Phusion Plus DNA Polymerase (250 μL)• 5X Phusion Plus Buffer (5 x 1.25 mL)• 5X Phusion GC Enhancer (4 x 1.25 mL)Store at –15°C to –25°C.
Polymerase:
Phusion Plus DNA Polymerase
Shipping Condition:
Dry Ice
Fidelity (vs. Taq):
>100X
No. of Reactions:
2000 Reactions
Reaction Format:
Separate components
GC-Rich PCR Performance:
High
Reaction Speed:
15 to 30 sec./kb
For Use With (Application):
Hot-start PCR, High-fidelity PCR
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Size (Final Product):
20 kb or less
Content And Storage: __
Polymerase: __
Shipping Condition: __
Fidelity (vs. Taq): __
No. of Reactions: __
Reaction Format: __
GC-Rich PCR Performance: __
Reaction Speed: __
For Use With (Application): __
Hot Start: __
Overhang: __
Size (Final Product): __
Content And Storage:
__
Polymerase:
__
Shipping Condition:
__
Fidelity (vs. Taq):
__
No. of Reactions:
__
Reaction Format:
__
GC-Rich PCR Performance:
__
Reaction Speed:
__
For Use With (Application):
__
Hot Start:
__
Overhang:
__
Size (Final Product):
__